Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)

Document Type: Research Paper

Authors

Department of Biology, Faculty of Agriculture, Saveh branch, Islamic Azad University, Saveh, Iran

Abstract

Cotton (Gossypium hirsutum L.) is a source of edible oil and fatty acids (FAs). In this study, embryos’ explants were cultured in MS medium with different concentration of  plant growth regulators (phytohormones) include BA, IAA, IBA, 2, 4- D and NAA, to finding the relationship of hormone and organogenesis with oil and FAs content. Oil was extracted with chloroform and methanol. FAs of oil were esterificated and methylated for GC-MS analysis. The seeds and shoots oil were 36.1 and 4.5%. Oil of regenerated shoots (33.3%) in T2 with 0.25 BA and 5 mgl-1 IAA was higher than to seeds, shoots and all regenerated organs.Unsaturated FAs increased in some treatments depending on the type of phytohormone and organogenesis.  Amounts of all FAs of regenerated root of T4 in BA/IBA were lesser than to other samples. Our results showed that Sahel cultivar of cotton can synthesize 12 types of FAs, but some of those FAs are made under certain conditions depending on the type and concentration of phytohormones and organogenesis.

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[1]     Allen Jeffrey, J., Kiran Kumar S., Antonynishanthraj, R., Arivoli N., and Balakrishnan P. 2017. Experimental analysis of cotton seed oil biodiesel in a compression ignition engine.Inter J Sci, Eng Techno Res (IJSETR), 6 (3): 425-430.

[2]     Dermaux, A., Sandra, P. and Ferraz V. 1999. Analysis of free fatty acids and fatty acid phenacyl esters in vegetable oils and margarine by capillary electrochromatography. Electrophoresis, 20: 74-79.

[3]     Finer, J.J. 1988. Plant regeneration from somatic embryogenic suspension cultures of cotton (Gossypium hirsutum). Plant Cell Rep, 7: 399-402.

[4]     Firoozabady, E. and DeBoer D.L. 1993). Plant regeneration via somatic embryogenesis in many cutlivars of cotton (Gossypium hirsutum L.) In vitro cell Dev. Biol, 29: 166-173.

[5]     Folch, J., Lees, M. and Soane G.H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem, 226: 497-509.

[6]     Gupta, S.K., Srivastava, A.K., Single, P.K. and Tuli R. 1997. In vitro proliferation of shoots and regeneration of cotton. Plant cell tissue and organ culture, 51: 149-152.

[7]     Hitchcock, C. and Hammond E.W. 1980. In developments in food analysis, 2nd ed. Ed by King RD. Applied Sci. London.

[8]     Hubbard, W.D., Prosser, A.R., Sheppard, A.J., Newkirk, D.R., Osgood, T. and Jones S.T. 1977. Individual lipids and proximate analysis of various foods. French fried potatoes from ten chain resturants. J Agric Food Chem, 25: 1280-1281.

[9]     Khan T., Singh, A.K. and.  Pant, R.C. 2006. Regeneration via somatic embryogenesis and organogenesis in different cultivars of cotton (Gossypium SPP.). In vitro Cell. Develop. Bio. Plant.  42(6): 498–501.

[10]  KDowd, M., LBoykin, D., William, R., Meredith, J.R., Todd Campbell, B. and Fred M. 2010. Fatty Acid Profiles of Cottonseed Genotypes from the National Cotton Variety Trials. The Journal of Cotton Science, 14:64–73.

[11]  Kulis, M.J., Hep, A.F., Pham, P.X., Ribita, D. and Bomana B.M.M. 2010. Extraction and characterization of lipids from Salicornia virginica and S. europaea. NASA/Technical Memorandum. Washington DC.

[12]  Lawhon, J.T., Cater, C.M. and Mattil K.F. 1977. Evaluation of the food potential of sixteen varieties of cottonseed. J Am Oil Chem Soc, 54:75–80.

[13]  Lukonge, E., Labuschagne, M.T. and Hugo, A. 2007. The evaluation of oil and fatty acid composition in seed of cotton accessions from various countries. J Sci Food Agri, 87:340–347.

[14]  Matthäus, B. and Özcan M.M. 2015.  Oil Content, Fatty Acid Composition and Distributions of Vitamin-E-Active Compounds of Some Fruit Seed Oils.  Antioxidants, 4: 124-133.

[15]  McCabe, D.E. and Martinell, B.J. 1993. Transformation of elite cotton cultivars via particle bombardment of meristems. Bio.technol,. 11: 596-598.

[16]  Murashige, T. and Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco cultures. Phyiol. Plant, 15:473-497.

[17]  Oo, K.C. and Stumpf, P.K. 1983. The metabolism of the germinating oil palm (Elaeis guineensis) seedling: In Vivo Studies. Plant Physiol, 73(4): 1033-1037.

[18]  Rashid, B., Husnain T. and Riazuddin, S. 2004. In vitro shoot tip culture of cotton (gossypium hirsutum).Pak J Bot, 36(4): 817-823.

[19]  Sacks, F.M. and Katan, M. 2002. Randomized clinical trials on the effects of dietary fat and carbohydrates on plasma lipoproteins and cardiovascular disease. Am. J Med, 113 (9 Suppl 2):13S–24S.

[20]  Saeed, N.A., Zafar, Y. and Malik K.A. 1997. A simple procedure of Gossypium meristem shoot tip cutlure. Plant Cell, Tissue and Organ Culture, 51: 201-207.

[21]  Shimizu, K., Ishikawa, N., Muranaka, S. and Tang, J.  2001. Digestion trials of mixed diet with salt wort (Salicornia herbacea L.) in goats. Jpn. Trop Agr, 45: 45-48.

[22]  Sunilkumar, G., Campbell, L.M., Puckhaber, L., Stipanovic, R.D. and Rathore K.S. 2006. Engineering cottonseed for use in human nutrition by tissue-specific reduction of toxic gossypol. Proc. Nat. Acad. Sci, 103:18054–18059.

[23]  Trolinder, N.L. and Xhixian C. 1989. Genotype specificity of the somatic embryogenesis response in cotton. Plant Cell Rep, 8: 133-136.

[24]  Yan-Xia, W., Xing-Fen, W., GGui-Yin, Z. and Gai- Ying, H. 2006. Somatic Embryogenesis and Plant Regeneration from Two Recalcitrant Genotypes of Gossypium hirsutum. Agri sci China 5(5):323- 329.

[25]  Zock, P.L., deVries, J.H. and Katan, M.B. 1994. Impact of myristic acid versus palmitic acid on serum lipid and lipoprotein levels in healthy women and men. Arterioscler Thromb Vasc Biol 14:567–575.