ORIGINAL_ARTICLE
Salt-related Genes Expression Pattern in Salt-Tolerant and Salt-Sensitive Cultivars of Cotton (Gossypium sp.) under NaCl Stress
Salinity is one of the most important limitation factors in development of agricultural products. Cotton has a relative tolerance to salinity; however, salinity reduces its growth during germination and seedling stages. In this research, split-factorial design of time based on randomized complete block design with 3 replications was used. The real-time PCR results for, root, stem, and leaves of 14-day cotton seedlings of tolerant (Sepid) and sensitive (Thermus14) cotton cultivars with salinity levels from 0 to 16 ds.m-1 were analyzed at three time points, namely 0, 7 and 14 days after salinity stress. Selected genes for Real Time PCR reaction in current study were selected using Cytoscape 3.3.0 software. Results showed that the selected genes GhERF2, GhMPK2, GhCIPK6, GbRLK, GhNHX1, GhGST, GhTPS1 and Gh14-3-3 have positively responded to salinity stress and their expression in the root was higher than in stem and leaf. Moreover, the expression of tolerant genotype (Sepid) was higher than the sensitive cultivar (Thermus 14) one, however, a slight increase in sensitive genotypes was observed in a number of genes (GhERF2 and GhGST) 14 days after starting the stress treatment.
https://www.jpmb-gabit.ir/article_29768_6e684826288e91ce07415b64455ced9a.pdf
2018-06-01
1
15
10.22058/jpmb.2018.75866.1151
abiotic stress
Real-time PCR
Salt tolerance
Nafise
Taghizadeh
nataghizadeh@gmail.com
1
Department of Plant Breeding and Biotechnology, Agricultural Sciences and Natural Resources University, Sari, Iran
AUTHOR
Gholam Ali
Ranjbar
ali.ranjbar@gmail.com
2
Department of Plant Breeding and Biotechnology, Agricultural Sciences and Natural Resources University, Sari, Iran
LEAD_AUTHOR
Ghorban Ali
Nematzadeh
gh.nematzadeh@sanru.ac.ir
3
Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University, Sari, Iran
AUTHOR
Mohammad Reza
Ramazani Moghaddam
rezaramezani@yahoo.com
4
Crop and Horticultural Science Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Mashhad, Iran
AUTHOR
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ORIGINAL_ARTICLE
Genetic Linkage Map of Glu-D1 and Allelic Variation of HMW Glutenin Subunits in Some Iranian Bread Wheat genotypes
High-molecular weight (HMW (glutenin subunits are encoded by the Glu-1 loci (Glu-A1, Glu-B1 and Glu-D1 (on the long arms of chromosomes 1A, 1B and 1D. In the present study, we constructed genetic linkage map of Glu-D1and then investigated the allelic variation of HMW glutenin subunits at Glu-A1 and Glu-D1 gene loci in 30 Iranian genotypes using Functional markers. Glu-D1 was located at 50.8 cM on chromosome 1D and it was tightly linked to wPt-3743 marker (>1 cM). QTL analysis using composite interval mapping detected one significant QTL for grain yield (GY) on chromosome 1D. This QTL (QYld.abrii-1D) was located between wPt-3743 and Glu-D1 gene locus. Allelic variation of HMW glutenin subunits showed the most frequent alleles were the null allele at Glu-A1and Dx2+Dy12 alleles at Glu-D1loci. The frequency of Null alleles or 1 and 2* were 40% and 60% respectively. Only 9 genotypes included allelic combination of Dx5+ Dy10 and the rest of genotypes had Dx2+Dy12 in the Glu-D1 locus. According to the Nei's genetic diversity index, alleles at Glu-A1 locus have more dispersion in genotypes compared to Glu-D1 locus. The cluster analysis of data based on the Simple Matching coefficient and UPGMC methods, classified the genotypes into four groups. Six genotypes including: Bezostaya, Tajan, Navid, Karaj1, Neyshabour, and Golestan had Ax2* and Dx5+ Dy10 subunits at Glu-A1 and Glu-D1 gene loci. Identification of genotypes with suitable allelic combinations can be used in breeding programs, especially in hybridization.
https://www.jpmb-gabit.ir/article_31697_3307a2a2e3e47975700fbc84378fd281.pdf
2018-06-01
16
23
10.22058/jpmb.2018.80555.1156
High-molecular weight (HMW (glutenin subunits
Genetic map
Allelic diversity
Bread wheat
Amin
Azadi
azadi.amin@gmail.com
1
Department of Plant Breeding, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran.
LEAD_AUTHOR
Saeedeh Khani
Bafrouei
saeedekhani1@gmail.com
2
Department of Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran
AUTHOR
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[16] Ma, W., Zhang, W. and Gale, K. 2003. Multiplex-PCR typing of high molecular weight glutenin alleles in wheat. Euphytica 134(1):51–60.
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[17] Bushuk, W. 1997. Wheat breeding for end poduct use. In: Wheat: Prospects for global imrovments. Developments in Plant Breedind. 6:203-211. The Netherland: Kluwer Academic Publishers.
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[18] Rabinovich, S.V. 1989. Composition of high molecular weight glutenin subunits connected with good quality in spring wheats and its distribution in different countries of world in: A.E. Slinkard (ed), Proc. 9th Intl. Wheat Genetics Symp. Proc. 9th Intl. 4:254-256.
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[20] Shahnejat-Bushehri, A.A., Gomarian, M. and Yazdi-Samadi B. 2006. The high molecular weight glutenin subunit composition in old and modern bread wheats cultivated in Iran. Australian Journal of Agricultural Research. 57(10):1109-1114.
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[21] Shan, X.Y., Clayshulte, S.R., Haley, S.D. and Byrne, P.F. 2007. Variation for glutenin and waxy alleles in the US hard winter wheat germplasm. Journal of Cereal Science. 45(2):199-208.
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[22] Nakamura, H. 2000. Allelic variation at high-molecular-weight glutenin subunit loci, Glu-A1, Glu-B1 and Glu-D1, in Japanese and Chinese hexaploid wheats. Euphytica. 112:187-193.
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[23] Zheng, S. 2007. Relationship of Glutenin Loci and Rye Translocations with Dough Mixing Properties of Wheat Grown in Colorado Environments. Dissertation. Colorado State University.
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[24] Izadi-Darbandi, A, Yazdi-Samadi, B., Shahnejat-Boushehri, A.A. and Mohammadi, M. 2010. Allelic variations in Glu-1 and Glu-3 loci of historical and modern Iranian bread wheat (Triticum aestivum L.) cultivars. Genetics.89:193-199.
24
[25] Deng, Z., Hu, S., Zheng, F., Chen, J., Zhang, X., Chen, J., Sun, C., Zhang, Y., Wang, S. and Tian, J. 2013. Genetic dissection reveals effects of interaction between high-molecular-weight glutenin subunits and waxy alleles on dough-mixing properties in common wheat. J. Genet. 92(1):69-79.
25
ORIGINAL_ARTICLE
Enhanced defense responses in Pythium ultimum-challenged cucumber plants induced by potassium phosphite
Pythium ultimum is one of the major causative agents responsible for damping off disease in cucumber plants. In the present study, the effect of potassium phosphite (KPhi) on defense response of P. ultimum-inoculated cucumber plants was investigated. Different plant growth parameters as well as chlorophyll a content were studied to evaluate the healing effects of KPhi. Furthermore, the expression pattern changes of a pathogenesis-related chitinase gene was analyzed via qPCR. Results revealed that KPhi treatment significantly increased growth parameters i.e. shoot length, diameter and mean leaf number in cucumber seedlings. KPhi treatment at 1 and 4 gL-1 caused 31.37% and 94.48% increase in shoot diameter respectively compared to control plants while shoot length of plant treated with 1 and 4 gL-1 KPhi were increased 72.14% and 78.85%, respectively compared to control plants. The chlorophyll a content as well as plant leaf number was significantly increased in plants treated with 1 or 4 gL-1 KPhi compared to control plants. It was interestingly revealed that KPhi application decreased Chitinase gene expression compared to control plants. The findings of the present study would be implemented for designing a controlling strategy to decrease the adverse effect of P. ultimum on cucumber plants.
https://www.jpmb-gabit.ir/article_29933_57df7a2ad54b4e5794d6445767677094.pdf
2018-06-01
24
33
10.22058/jpmb.2018.74694.1147
Pythium ultimum
potassium phosphite
chlorophyll a
chitinase
qPCR
Maryam
Mofid Nakhaei
mofid.nakhaei@yahoo.com
1
Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
AUTHOR
Vahid
Abdossi
abdossi@srbiau.ac.ir
2
Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
AUTHOR
Ali
Dehestani
a.dehestani@gmail.com
3
Molecular Genetics Dept, Genetics and Agricultural Biotechnology Institute of Tabrestan, Sari Agricultural Sciences and Natural resources university, Sari, Iran
LEAD_AUTHOR
Hematollah
Pirdashti
h.pirdashti@sanru.ac.ir
4
Department of Agronomy, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
AUTHOR
Valiollah
Babaeizad
v.babaeizad@sanru.ac.ir
5
Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
AUTHOR
[1] Abbasi, P.A. and Lazarovits, G. 2006. Seed treatment with phosphonate (AG3) suppresses Pythium damping-off of cucumber seedlings. Plant Dis.,90: 459-464.
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[4] Barzegargolchini, B., Movafeghi, A., Dehestani, A. and Mehrabanjoubani, P. 2017. Increased cell wall thickness of endodermis and protoxylem in Aeluropus littoralis roots under salinity: the role of LAC4 and PER64 genes. J Plant Physiol., 218: 127-134.
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[5] De Lorenzo, G., D Ovidio, R. and Cervone, F. 2001. The role of polygalacturonase-inhibiting
5
proteins (PGIPs) in defense against pathogenic fungi. Annual Rev Phytopathol., 39: 313-335.
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[7] Dolatabadi, B., Ranjbar, G., Tohidfar, M. and Dehestani, A. 2014. Genetic transformation of Tomato with three pathogenesis-related protein genes for increased resistance to Fusarium oxysporum f. sp. Lycopersici. J Plant Mol.r Breed., 2: 1-11.
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[13] Kasprzewska, A. 2003. Plant chitinases: Regulation and Function. Cell. Mol. Biol. Lett., 8: 809-824.
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[14] Khosravi, F., Gharanjik, S. and Dehestani, A. 2017. Molecular responses of Phytophthora capsici-challenged cucumber (Cucumis sativus L.) plants as influenced by resistance inducer application. J Plant Mol. Breed., 5: 1-10.
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[15] King, M., Reeve, W. and Vander, A. 2010. Defining the phosphite-regulated transcriptome of the plant pathogen Phytophthora cinnamomi. Mol. Genet. Genom., 284: 425-435.
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[19] Lobato, M.C., Olivieri, F.P., Daleo, G.R. and Andreu, A.B. 2010. Antimicrobial activity of phosphites against different potato pathogens. J Plant Dis. and Protec., 117: 102-109.
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[20] Machinandiarena, M.F., Lobato, M.C., Feldman, M.L., Daleo, G.R. and Andreu, A.B. 2012. Potassium phosphite primes defense responses in potato against Phytophthora infestans. J Plant Physiol., 169:1417-1424.
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[21] Manna, M., Islam, T., Kaul, T., Reddy, C.S., Fartyal, D., James, D. and Reddy, M.K. 2015. A comparative study of effects of increasing concentrations of phosphate and phosphite on rice seedlings, Acta Physiol.Plant., 37: 258-163.
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[22] Mofidnakhaei, M., Abdossi, V., Dehestani, A., Pirdashti, H. and Babaeizad, V. 2016. Potassium phosphite affects growth, antioxidant enzymes activity and alleviates disease damage in cucumber plants inoculated with Pythium ultimum. Arch. Phytopathol. Plant Protec., 49: 207-221.
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[23] Moradi, N., Rahimian, H., Dehestani, A. and Babaeizad, V. 2016. Cucumber Response to Sphaerotheca fuliginea: Differences in antioxidant enzymes activity and pathogenesis-related gene expression in susceptible and resistant genotypes. Journal of Plant Molecular Breeding, 4: 33-40.
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[24] Moor, U., Poldma., P., Tonutare., T., Karp, K., Starast, M. and Vool, E. 2009. Effect of phosphite fertilization on growth, yield and fruit composition of strawberries. Scien. Hort., 119: 264-269.
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[25] Neycee, M. A., Nematzadeh, G.A., Dehestani, A. and Alavi, M. 2012a. Assessment of antifungal effects of shoot extracts in chinaberry (Melia azedarach) against 5 phytopathogenic fungi. Intl. J Agron. Plant Prod., 4: 474-477.
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[26] Neycee, M. A., Nematzadeh, G.A., Dehestani, A. and Alavi, M. 2012b. Evaluation of antibacterial effects of chinaberry (Melia azedarach) against gram-positive and gram-negative bacteria. Intl J Agric. crop scie., 4: 709-712.
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[27] Olivieri, F.P., Feldman, M.L., Machinandiarena, M.F., Lobato, M.C., Caldiz, D.O., Daleo, G.R. and Andreu, A.B. 2012. Phosphite applications induce molecular modifications in potato tuber periderm and cortex that enhance resistance to pathogens. Crop Protec., 32: 1-6.
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[28] Oyarburo, N.S., Machinandiarena, M.F., Feldman, M.L., Daleo, G.R., Andreu, A.B. and Olivieri, F.P. 2015. Potassium phosphite increases tolerance to UV-B in potato, Plant physiol. biochem., 88: 1-8.
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[33] Ramezani, M., Rahmani, F. and Dehestani, A. 2017b. The effect of potassium phosphite on PR genes expression and the phenylpropanoid pathway in cucumber (Cucumis sativus) plants inoculated with Pseudoperonospora cubensis. Sci. Hort., 225: 366-372.
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[35] Ramezani, M., Ramezani, F., Rahmani, F. and Dehestani, A. 2018. Exogenous potassium phosphite application improved PR-protein expression and associated physio-biochemical events in cucumber challenged by Pseudoperonospora cubensis. Sci. Hort., 234: 335-343.
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43
ORIGINAL_ARTICLE
QTL analysis of yield and yield related traits in bread wheat under salt-stress conditions
In order to identify yield and yield component QTLs under control and salt-stress conditions, a population of 254 recombinant inbred lines (RILs), derived from a cross between two bread wheat cultivars, (Roshan / Sabalan), was assessed. Parents and their 254 recombinant inbred lines (RILs) were evaluated in an alpha-lattice design with two replications in two control and saline environments of Yazd in 2011-2012 cropping season. Yield and yield-related traits were evaluated at harvest time. The genotyping was carried out using SSR and DArT markers. A, B and D genomes were covered by 411.8, 620.4 and 67.5 cM, respectively. Also, a total of 48 QTLs were detected on 11 chromosomes for grain yield, biological yield, harvest index, thousand-kernel weight, grain number per spike, spike weight and spikelet number per spike. Roshan (salt tolerance) alleles were associated with an increase yield under saline conditions. SSR markers including gwm146, gwm577, gwm249 (on chromosomes 2A and 7B) were tightly associated with different QTLs. The major effect QTLs were located on chromosomes 1A and 7B for grain yield, harvest index and spike weight, which were explained 10.2%, 12.98% and 29 % of the total phenotypic variance, respectively. These QTLs and markers could be suitable for marker-assisted selection and gene stacking techniques. Moreover, co-located QTLs were detected on chromosome 2B for evaluated traits.
https://www.jpmb-gabit.ir/article_33437_82c6885c3557c2bc00ebb0c9a14fac91.pdf
2018-06-01
34
43
10.22058/jpmb.2018.77518.1152
Bread wheat
QTL
Salt- stress
Grain yield
Mahnaz
Rahmati
avinmahnaz@gmail.com
1
Seed and Plant Improvement Research Department, Lorestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Khorramabad, Iran
LEAD_AUTHOR
Mohsen
Mardi
mardi@abrii.ac.ir
2
Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
AUTHOR
Mohammad-Reza
Naghavi
mnaghavi@ut.ac.ir
3
Department of Plant Breeding, University of Tehran, Karaj, Iran
AUTHOR
Eslam
Majidi Heravan
majidi_e@yahoo.com
4
Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
AUTHOR
Babak
Nakhoda
b.nakhoda@abrii.ac.ir
5
Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
AUTHOR
Amin
Azadi
azadi.amin@gmail.com
6
Department of Plant Breeding, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran
AUTHOR
Ghasem
Mohammadi-Nejad
mohammadinejad@yahoo.com
7
Agronomy and Plant Breeding Department, Faculty of Agriculture, Shahid-Bahonar University of Kerman
AUTHOR
[1] Alheit, K. V., Busemeyer, L., Liu, W., Maurer, H. P., Gowda, M., Hahn, V., Weissmaun, S., Ruckelshausen, A., Reif, J. C. and Wϋrschum, T. 2014. Multiple-line cross QTL for biomass yield and plant height in triticale (×TriticosecaleWittmack). TheorAppl Genet, 127:251-260.
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[8] Cuthbert, J. L., Somers, D. J., Brule-Babel, A. L., Brown, P. D. and Crown, G. H. 2008: Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (TriticumaestivumL.). TheorAppl Genet, 117:595-608.
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[17] Gupta, P. K., Balyan, H. S., Edwards, K. J., Isaac, P., Korzun, V., Röder, M. S., Gautier, M. F., Joudrier, P., Schlatter, A. R. and Dubcovsky, J. 2002. Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. TheorAppl Genet, 105:413-422.
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[35] Poustini, K. and Siosemardeh, A. 2004. Ion distribution in wheat cultivars in response to salinity stress. Field Crops Res, 85:125-133.
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[36] Quarrie, S. A., Steed, A., Calestani, C., Semikhodskii, A., Lebreton, C., Chinoy, C., Steele, N., Pljevljakusic, D., Waterman, E., Weyen, J., Schondelmaier, J., Habash, D. Z., Farmer, P., Saker, L., Clarkson, D. T., Abugalieva, A., Yessinbekova, M., Turuspekov, Y., Abugalieva, S., Tuberosa, R., Sanguineti, M. C., Hollington, P. A., Aragues, R., Royo, A. and Dodig, D. 2005. A high-density genetic map of hexaploid wheat (TriticumaestivumL.) from the cross Chinese Spring X SQ1 and its use to compare QTLs for grain yield across a range of environments. TheorAppl Genet, 110:965-990.
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38
[39] Torada, A., Koike, M., Mochida, K. and Ogihara, Y. 2006. SSR-based linkage map with new markers using an intraspecific population of common wheat. TheorAppl Genet, 112:1042–1051
39
[40] Wang, R. X., Zhang, X. Y., Wu, L., Wang, R., Hai, L., Yan, C. S., You, G. X. and Xiao, S. H. 2008. QTL mapping for grain filling rate and thousand-grain weight in different ecological environments in wheat. ActaAgron Sin, 34:1750-1756.
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41
ORIGINAL_ARTICLE
Molecular cloning and in-silico analysis of Ramy3D promoter and 5′ untranslated region from an Iranian rice (Oryza sativa L.) cultivar “NEMAT”
The regulatory sequence of rice alpha amylase 3D gene (Ramy3D) is amongst the most successful expression systems used for recombinant protein expression in plants. In the current study a 995 bp fragment consisting of Ramy3D promoter and its 5′ untranslated region was amplified from the genomic DNA of an Iranian rice cultivar ″Nemat″, using polymerase chain reaction. The amplified fragment was ligated into the pTG19-T vector and the cloned fragment was sequenced. For in silico characterization, the rice specific consensus sequences of TATA-box and YR Rule motifs were scanned against the cloned fragment sequence using FIMO program and the cis acting elements existing in the promoter region were investigated using PlantCare database. A TATA-box motif with the rice specific pattern was identified at upstream position of the transcription start site. The identification of TATA-box in Ramy3D promoter is consistent with its metabolic and tissue specific regulation manner. Several cis regulatory motifs responsible for the metabolic and hormonal regulation of Ramy3D gene were identified including ABRE, G-Box, GC-box, GATA motif and TATCCA T/C motif. In addition, several motifs involved in response to various stimuli such as plant hormones, light and biotic and abiotic stresses were identified which include circadian motif, as-2-box, WUN-motif, TGACG-motif, Skn-1 motif, O2-site, MBS, LAMP-element, I-box, HSE, GCC Box, GATT motif, CGTCA-motif and GAG-motif.
https://www.jpmb-gabit.ir/article_34694_8c05bfb99491e7a67ecebd78d93aed41.pdf
2018-06-01
44
52
10.22058/jpmb.2019.92898.1172
Rice
Amylase
Ramy3D
Promoter
Regulatory cis elements
5′ untranslated region
Meysam
Bastami
meysambastami@yahoo.com
1
Department of biotechnology, Faculty of agriculture and natural resources, Imam Khomeini international university, Qazvin, Iran
AUTHOR
Hosseini
Ramin
raminh_2001@yahoo.com
2
Dept. of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University (IKIU), Qazvin, 34149-16818, IR. of Iran.
LEAD_AUTHOR
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51
ORIGINAL_ARTICLE
Influence of Agrobacterium rhizogenes strains on hairy roots induction in Trigonella foenum-graecum L. and secondary metabolites production
Fenugreek(Trigonella foenum-graecum L.) is a rich source of important medicinal metabolites. This plant belongs to the Fabaceae family. Induced hairy roots by Agrobacterium rhizogenes are a suitable tissue for the production of secondary metabolites, due to the stability and high production of roots without phytohormone in a short time. Different strains of Agrobacterium rhizogenes (A4, ATCC11325 and ATCC15834) were evaluated for induction of transformed hairy roots in T. foenum-graecum L. using seedling explants. The application of hairy root culture may become an alternative method for increase secondary metabolites. Transgenic status of the roots was confirmed by PCR using rolB specific primers. All of the A. rhizogenes strains led to hairy roots induction. The maximum frequency of transformation (97.87%) was obtained using A4 strain in 7-days-old seedling. The 7-days-old explants were inoculated using A4 strain result in highest fresh (0.166 g) and dry (0.080 g) weight of roots. The explants were inoculated by ATCC11325 strain produced hairy roots with highest amount of total phenol (8.113 mg/g DW) and flavonoid content (3.215 µg/g DW).
https://www.jpmb-gabit.ir/article_35069_99068b9a49064658d249d3187df4d124.pdf
2018-06-01
53
60
10.22058/jpmb.2019.100761.1177
Fenugreek
Medicinal plant
Polymerase chain reaction
rolB
Neda
Tariverdizadeh
n.tarverdi.1991@gmail.com
1
Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
AUTHOR
Mehdi
Mohebodini
mohebodini@uma.ac.ir
2
Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
LEAD_AUTHOR
Esmaeil
Chamani
echamani@uma.ac.ir
3
Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
AUTHOR
Asghar
Ebadi
asghar_ebadi@uma.ac.ir
4
Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
AUTHOR
[1] Aasim, M., Hussain, N., Umer, E.M., Zubair, M., Hussain, S.B., Saeed, S.H., Rafique, T.S. and Sancak, C. 2010. In vitro shoot regeneration of fenugreek (Trigonellafoenum-graecum L.) using different cytokinins. Afr. J. Biotechnol, 9:( 42) 7174-7179.
1
[2] Akbarian, R., Hasanloo, T. and Khosroshahi, M. 2011. Evaluation of trigonelline production in Trigonella foenum-graecum hairy root cultures of two Iranian masses. Plant Omics J, 4: 408-412.
2
[3] Al-Mahdawe, M.M., Al-Mallah, M.K. and Al-Attrakchii, A.O. 2013. Genetically transformed hairy roots producing agropine induced on Trigonella foenum-graecum L. plant by Agrobacterium rhizogenes 1601. J of Biotechnology Research Center, 7: 91-98.
3
[4] Banihashemi, O., Khavari, R.A., Yassa, N. and Najafi, F. 2015. Induction of hairy roots in Atropa komarovii using Agrobacterium rhizogenes. Indian Journal of Fundamental and Applied Life Sciences, 5(3): 2014-2020.
4
[5] Bertani, G. 1952. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol, 62: 293–300.
5
[6] Chang, C., Yang, M., Wen, H. and Chern, J. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. JFDA, 10: 178-182.
6
[7] Christey, M.C. and Braun, R.H. 2005. Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes – mediated transformation. Transgenic Plants: Methods and Protocols, 286: 47–60.
7
[8] Doyle, J.J. and Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15.
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[9] Gamborg, O.L., Miller, R.A. and Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50: 151–158.
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[10] Georgiev, M., Pavlov, A. and Bley, T. 2007. Hairy root type plant in vitro systems as sources of bioactive substances. Applied Microbiology and Biotechnology, 74: 1175–1185.
10
[11] Kabirnotaj, S., Zolalla, J., Nematzadeh, G. and SHokri, E. 2013. Optimization of hairy root culture establishment in Chicory plants (Cichorium intybus) through inoculation by Agrobacterium rhizogenes. J. Agri. Biotec, 4(2): 61-75.
11
[12] Kayser, O. and Quax, W.G. 2007. Medicinal plant biotechnology.Vol. 1, WILEY-VCH Verlag GmbH & Co., Weinheim, 604 pp.
12
[13] Kim, S.I., Veena, A. and Gelvin, S.B. 2007. Genome-wide analysis of Agrobacterium T-DNA integration sites in the Arabidopsis genome generated under non-selective conditions. The plant Journal, 51: 779-791.
13
[14] Manuhara, Y.S.W., Kristanti, A.N., Utami, E.S.W., Yachya, A. 2015. Effect of sucrose and potassium nitrate on biomass and saponin content of Talinum paniculatum Gaertn. Hairy root in balloon-type bubble bioreactor. J Trop Biomed, 5(12): 1027- 1032.
14
[15] Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G. 2005. Determination of the total phenolic, flavonoid and pralin contents in Burkina Fasan honey, as well as their scavenging activity. Food Chemistry,91: 571-577.
15
[16] Merkli, A., Christen, P. and Kapetanidis, I. 1997 Production of diosgenin by hairy root cultures of Trigonella foenum.graecum L. Plant Cell Reports, 16: 632-636.
16
[17] Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473- 497.
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[18] Peraza-Luna, F., Rodrı´guez-Mendiola, M., Arias-Castro, C., Bessiere, J.M. and Calva-Calva, g. 2001. Sotolone production by hairy root cultures of Trigonella foenum-graecum in Airlift with Mesh Bioreactors. J. Agric. Food Chemistry, 49: 6012-6019.
18
[19] Qaderi, A., Akbari, Z., Kalateh-jari, S, Fatehi, F., Tolyat, M., Jalali Moghadam, M. and Naghdi Badi, H. 2014. Improving Trigonelline Production in Hairy Root Culture of Fenugreek (Trigonella foenum-graecum). Journal of Medicinal Plants,15: 73-80.
19
[20] Sevon, N. and Oksman-Caldentey, K.M. 2002. Agrobacterium rhizogenes-Mediated Transformation: Root Cultures as a Source of Alkaloids. Planta Med, 68(10): 859-868.
20
[21] Shahabzadeh, Z., Heidari, B. and Faramarzi Hafez, R. 2013. Induction of Transgenic Hairy Roots in Trigonella foenumgraceum Co-cultivated with Agrobacterium Rhizogenes Harboring a GFPGene. Journal of Crop Science and Biotechnology, 16 (4): 263- 268.
21
[22] Sharafi, A., Hashemi, Sohi H., Mirzaee, H. and Azadi, P. 2014. In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss. Physiol Mol Biol Plants, 20(4): 487–494.
22
[23] Sharafi, A., Hashemi, S. H., Mousavi, A., Azadi, P., Razavi, K. and Ntui, V.O. 2012. A reliable and efficient protocol for inducing hairy roots in Papaver bracteatum. PCTOC, 113: 1-9.
23
[24] Srivastava, V., Kaur, R., Chattopadhyay, A.K. and Banerjee, S. 2013. Production of industrially important cosmaceutical and pharmaceutical derivatives of betuligenol by Atropa belladonna hairy root mediated biotransformation. Industrial Crops and Products, 44: 171–175.
24
[25] Valimehr, S., Sanjarian, F., Hashemi sohi, H., Sharafi, A. and Sabouni, F. 2014. A reliable and efficient protocol for inducing genetically transformed roots in medicinal plant Nepeta pogonosperma. Physiol Mol Biol Plants, 20(3): 351–356.
25
ORIGINAL_ARTICLE
Assessment of the efficiency of hairy roots induction using soybean, sugar beet and tobacco explants
Agrobacterium-mediated gene transfer method is one of the used methods for genetic transformation in the plant regeneration program. Transformation efficiency can be optimized depending on the strain of bacteria, the genotype of plant and conditions of growth. In this study, the gfp gene was transferred into sugar beet, tobacco, and soybean by Agrobacterium rhizogenes strain AR15834. The effects of bacterial concentrations, antibiotic concentrations and the types of explants and genotypes on the gene transfer efficiency and transgenic hairy roots production were investigated. The explants were inoculated with the bacteria at the adjusted concentrations and two days after the transformation, the explants were transferred to a solid MS medium containing different concentrations of kanamycin antibiotic. According to the results and the examined factors, the optimal conditions to achieving of the maximum production of transgenic hairy roots included bacterial concentration with OD600 = 0.2, cotyledon explant, 50 mg/L kanamycin concentration and Djakel genotype for soybean; bacterial concentration with OD600 = 0.2, leaf with petiole explant and SBSI004 genotype for sugar beet, and bacterial concentration with OD600nm = 0.8 and 100 mg/L kanamycin concentration for tobacco.
https://www.jpmb-gabit.ir/article_35271_228a8b4a5d74d62f2eca0d66ec14c45c.pdf
2018-06-01
61
72
10.22058/jpmb.2019.101418.1178
gfp
transgenic hairy roots
Soybean
sugar beet
Tobacco
Nahid
Sadeghi Ghahderijani
ndsadeghi90@gmail.com
1
Institute of Biotechnology, Shiraz University, Shiraz, Iran.
AUTHOR
Ali
Niazi
niazi@shirazu.ac.ir
2
Institute of Biotechnology, Shiraz University, Shiraz, Iran.
LEAD_AUTHOR
Esmaeil
Ebrahimie
ebrahimie@shirazu.ac.ir
3
Institute of Biotechnology, Shiraz University, Shiraz, Iran.
AUTHOR
Ali
Moghadam
4
Institute of Biotechnology, Shiraz University, Shiraz, Iran.
AUTHOR
Mohammad Sadegh
Taghizadeh
ms.taghizadeh69@gmail.com
5
Institute of Biotechnology, Shiraz University, Shiraz, Iran.
AUTHOR
[1] Srivastava, S. and A.K. Srivastava, Hairy root culture for mass-production of high-value secondary metabolites. Critical Reviews in Biotechnology, 2007. 27(1): p. 29-43.
1
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