ORIGINAL_ARTICLE
Study of genetic variation of some Iranian rice (Oryza sativa) genotypes based on morphological traits, physicochemical properties and molecular markers
Assessment of genetic diversity and individual relationships in rice (Oryza sativa) germplasm collections seems to be necessary for future rice breeding program. In order to understand genetic relationships of 30 rice genotypes, nine morphological traits, seven physicochemical properties and twelve RAPD primers were used for study of 30 rice genotypes. Among morphological traits, number of unfilled grain, number of tiller, number of filled grain and plant height had the highest CV value that indicated the high range of genetic diversity for studied genotypes. Pairwise correlation of morphological traits and physicochemical properties showed plant height had a strong positive correlation with panicle length (r = 0.721, P< 0.0001). Also, ratio of white rice to paddy rice and milling ratio had a negative correlation with plant height and 1000-grain weight, respectively. Cluster analysis of physicochemical properties and morphological traits grouped all genotypes into three main clusters. A total of 105 obtained RAPD bands, a number of 35 bands were polymorphs which range 7 to 19 bands per primer. OPB-14 and OPH-12 primers shown that lowest and the highest number of bands per primers, respectively. Cluster analysis of molecular data based on UPGMA algorithm and Jaccard's similarity coefficient grouped 30 rice genotypes into three clusters. The findings of this study might provide valuable information about local rice cultivar relationships in terms of their genetic distance, and can be useful in rice breeding program.
https://www.jpmb-gabit.ir/article_31618_efd2779898044dd4bff686c880130adc.pdf
2018-12-01
1
9
10.22058/jpmb.2018.86951.1159
Oryza sativa
genetic variation
Morphological trait
Physicochemical property. RAPD
Aliakbar
Babajanpour
ababajanpour@yahoo.com
1
Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran, P. O. Box 578
AUTHOR
SeyedHamidreza
Hashemipetroudi
irahamidreza@yahoo.com
2
Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran, P. O. Box 578
LEAD_AUTHOR
Mostafa
Haghpanah
masoudhgh@gmail.com
3
Department of Plant breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran, P. O. Box 578
AUTHOR
[1] Alizadeh, M., Nematzadeh, G., Ebrahimi, M., and Hashemi, S. 2013. Fingerprinting of Some Rice (Oryza sativa L.) Germplasm via AFLP Markers. Crop Biotech., 3(4): 53-60.
1
[2] Allhgholipour, M., Farshdfar, E., and Rabiei, B. 2014. Molecular characterization and genetic diversity analysis of different rice cultivars by microsatellite markers. Genetika, 46(1): 187-198.
2
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3
[4] Babaei, A., Nematzadeh, G.-A., and Hashemi, H. 2011. Molecular RAPD markers analysis of Sange-tarom and Taromhashemi cultivars (Oryza sativa L.) in M2 population. Annals Biol. Res, 2(4): 24-30.
4
[5] Babaei, A., Nematzadeh, G.A., Avagyan, V., and Hashemi-Petrodi, S.H. 2010. Radio sensitivity studies of morpho-physiological characteristics in some Iranian rice varieties (Oryza sativa L.) in M1 generation. African Journal of Agricultural Research, 5(16): 2124-2130.
5
[6] Bagheri, N., Babaeian, J.N., and HASAN, N.E. 2008. Genetic diversity of Iranian rice germplasm based on morphological traits.
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[7] Bagheri, N., Babaeian, N., and nataj, H. 2008. Genetic diversity of Iranian rice germplasm based on morphological traits. Iranian journal of field crops research, 6(2): 235-243.
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[10] Haque, M., Islam, S., Banik, M., Khalequzzaman, M., Siddiquee, M., and Mian, M. 2013. Physicochemical and cooking properties of local aromatic rice gerplasm in Bangeladesh. Eco-friendly Agril. J., 6(11): 243-248.
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[11] Hashemi-Petroudi, S.H., Maibody, S.A.M.M., Nematzadeh, G.A., and Arzani, A. 2010. Semi-random PCR markers for DNA fingerprinting of rice hybrids and theirs corresponding parents. African Journal of Biotechnology, 9(7): 979-985.
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[12] Hashemi, S.H., Mirmohammadi-Maibody, S.A.M., Nematzadeh, G.A., and Arzani, A. 2009. Identification of rice hybrids using microsatellite and RAPD markers. African Journal of Biotechnology, 8(10).
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[13] Huang, B.E., George, A.W., Forrest, K.L., Kilian, A., Hayden, M.J., Morell, M.K., and Cavanagh, C.R. 2012. A multiparent advanced generation inter‐cross population for genetic analysis in wheat. Plant biotechnology journal, 10(7): 826-839.
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[14] Huang, Y.-F., Poland, J.A., Wight, C.P., Jackson, E.W., and Tinker, N.A. 2014. Using genotyping-by-sequencing (GBS) for genomic discovery in cultivated oat. PloS one, 9(7): e102448.
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[15] Jahani, M., Nematzadeh, G., Dolatabadi, B., Hashemi, S.H., and Mohammadi-Nejad, G. 2014. Identification and validation of functional markers in a global rice collection by association mapping. Genome, 57(6): 355-362.
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[16] Jahani, M., Nematzadeh, G., Mohammadi-Nejad, G., Hashemi, S., Dolatabadi, B., and Hajipoor, A. 2013. Grain size diversity in rice (Oryza sativa L.) genotypes. International Journal of Agronomy and Plant Production, 4(8): 2024-2029.
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[18] Kishine, M., Suzuki, K., Nakamura, S., and Ohtsubo, K.i. 2008. Grain qualities and their genetic derivation of 7 new rice for Africa (NERICA) varieties. Journal of agricultural and food chemistry, 56(12): 4605-4610.
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[20] Little, R.R. 1958. Differential effect of dilute alkali on 25 varieties of milled white rice. Cereal Chem., 35: 111-126.
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[21] Mahjoob, B., Zarini, H., Hashemi, S., and Shamasbi, F. 2016. Comparison of ISSR, IRAP and REMAP markers for assessing genetic diversity in different species of Brassica sp. Russian Journal of Genetics, 52(12): 1272-1281.
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[22] Majzoobi, M. and Farahnaky, A. 2010. The Physicochemical Properties of Starch Component of six Iranian Rice Cultivars. Iran Agricultural Research, 27(1.2): 113-122.
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[29] Parsaeian, M., Mirlohi, A., and Saeidi, G. 2011. Study of genetic variation in sesame (Sesamum indicum L.) using agro-morphological traits and ISSR markers. Russian journal of genetics, 47(3): 314.
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37
ORIGINAL_ARTICLE
Analysis of genetic diversity among male and female pistachio genotypes using start codon targeted (SCoT) makers
Precise investigation of genetic diversity by means of novel molecular tools has made it possible to identify the superior genotypes among various male and female pistachio populations. Cytogenetic studies have shed light on the possible presence of distinct sex chromosomes in male and female genotypes. In this study, 22 start codon targeted (SCoT) primers were used to investigate the genetic diversity of 22 male genotypes and 22 female cultivars of pistachio. A total of 434 loci were produced that 339 loci were polymorphism. The average value of polymorphic information content (PIC), marker index (MI), and resolving power (Rp), ranged from minimum 10, 0.5, and 1, to maximum 31, 11.40, and 17.86% subsequently. The genetic similarity between genotypes, were calculated using Jaccard's coefficient, ranged from 35 to 66%. The cluster analysis divided pistachio genotypes into six groups, and could efficiently differentiate the male and female genotypes. Analysis of molecular variance (AMOVA) classified the total diversity into intra- and inter- population diversities with a high genetic variation (92%) within populations. This study reveals that SCoT marker is a useful and valuable molecular tool to separate male and female pistachios and to determine the genetic diversity among the populations.
https://www.jpmb-gabit.ir/article_35434_b8e05a0676907573e66b4c9609778a1a.pdf
2018-12-01
10
18
10.22058/jpmb.2019.108160.1183
cluster analysis
genotyping
Molecular marker
molecular variance
Khalil
Malekzadeh
kh.malekzadeh@vru.ac.ir
1
Department of Genetics and Crop Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan,Iran.
LEAD_AUTHOR
Mohsen
Mahmoodnia
m.mahmoodnia@vru.ac.ir
2
Department of Genetics and Crop Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.
AUTHOR
Fatemeh
Farzad Amirebrahimi
fatemehfarzad93@gmail.com
3
Department of Genetics and Crop Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.
AUTHOR
[1] Basr Ila, H., Kafkas, S. and Topaktas, M. 2003. Chromosome numbers of four Pistacia (Anacardiaceae) species. J Hortic Sci Biotech, 78(1):35-38.
1
[2] Collard, B.C.Y. and Mackill, D.J. 2009. Start codon targeted (SCoT) polymorphism: A simple, novel DNA marker technique for generating gene- targeted markers in plants. Plant Mol Biol Rep, 27:86-93.
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[3] Crane, J.C. and Iwakiri, B.T. 1979. Is pistachio nut development influenced by pollen source? California Pistachio Industry Annual Report, 35-37.
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[4] Doyle, J. and J. Doyle, J.L. 1987. A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull, 19: 11-15.
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[5] Farzad Amirebrahimi, F, Mahmoodnia Meimand, M., Karimi, H.R., Malekzadeh, K. and Tajabadipour, A. 2017. Genetic diversity assessment of male and female pistachio genotypes based on ISSR markers. JPMB, 5(1): 31-39.
5
[6] Fazeli, F., Cheghamirza, K. 2011. Evaluation of genetic diversity in Iranian chickpea (Cicer arietinum L.) Accessions using ISSR markers. MGJ, 6(2):97-104 (Abstract in English).
6
[7] Guo, Z. H., Fu, K. X., Zhang, X. Q., Bai, S. Q., Fan, Y., Peng, Y., Huang, L. K., Yan, Y. H., Liu, W. and Ma, X. 2014. Molecular insights into the genetic diversity of Hemarthria compressa germplasm collections native to southwest China. Molecules, 19: 21541-21559.
7
[8] Hajizadeh Hosseinabadi, M., Karimi, H., Dashti, H., Shamshiri, M. and Tajabadipour, A. (2013). Assessment of genetic diversity among some male and female pistachio (Pistacia vera L.) genotypes using RAPD marker, Applied Crop Breeding, 1(1): 23-32. (Abstract in English)
8
[9] Kafkas, S., Kafkas, E. and Perl-Treves, R. 2002. Morphological diversity and a germplasm survey of three wild Pistacia species in Turkey. Genet Resour Crop Ev, 3: 261–270.
9
[10] Kafkas, S., Khodaeiaminjan, M., Güney, M. and Kafkas, E. 2015. Identification of sex-linked SNP markers using RAD sequencing suggests ZW/ZZ sex determination in Pistacia vera L. BMC Genom, 16(1), p.98.
10
[11] Kafkas, S. and Perl-Treves, R. 2002. Inter-specific relationships in the genus Pistacia L. (Anacardiaceae) based on RAPD fingerprints. Hort Sci, 37: 168-171.
11
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12
[13] Mousapour Gorji, A., Poczai, P., Polgar, Z. and Taller, J. 2011. Efficiency of arbitrarily amplified dominant markers (SCOT, ISSR and RAPD) for diagnostic fingerprinting in tetraploid Potato. AJPR, 88: 226-237.
13
[14] Pazouki, L., Mardi, M., Salehi Shanjani, P., Hagidimitriou, M., Pirseyedi, S.M., Naghavi, M.R., Avanzato, D., Vendramin, E., Kafkas, S., Ghareyazie, B., Ghaffari, M. R. and Khayam Nekoui, S.M. 2010. Genetic diversity and relationships among Pistacia species and cultivars. Conserv Genet, 11:311-318.
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[15] Peakall, R.O.D. and Smouse, P.E. 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes, 6(1): 288-295.
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[16] Perry, A.L., Worthington, T., Hilton, A.C., Lambert, P.A., Stirling, A.J. and Elliott
16
T.S.J. 2003. Analysis of clinical isolates of Propionibacterium acnes by
17
optimised RAPD. FEMS Microbiol Lett, 228:51–5.
18
[17] Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalski, A. 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed, 2: 225-238.
19
[18] Prevost, A. And Wilkinson, M. 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet, 98: 107-112.
20
[19] Shahlaei, A., Torabi, S. and Khosroshahli, M. 2014. Efficiacy of SCoT and ISSR marekers in assesment of tomato (Lycopersicum esculentum Mill) genetic diversity. IJB, 2:14-22.
21
[20] Sola-Campoy, P.J., Robles, F., Schwarzacher, T., Rejón, C.R., de la Herrán, R. and Navajas-Pérez, R., 2015. The molecular cytogenetic characterization of pistachio (Pistacia vera L.) suggests the arrest of recombination in the largest heteropycnotic pair HC1. PLoS ONE, 10(12): p.e0143861.
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[21] Sorkheh, k., Amirbakhtiar, N. and Ercisli, S. 2016. Potential Start Codon Targeted (SCoT) and Inter-retrotransposon Amplified Polymorphism (IRAP) markers for evaluation of genetic diversity and conservation of wild Pistacia species population. Biochem Genet, 4: 368–387.
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[22] Taghizad, A., Ahmadi, J., Haddad, R. and Zarrabi, M. 2012. Study of Genetic Diversity in Iranian Pistachio Cultivars with Inter- microsatellite ISSR Markers. J Hortic Sci, 4:453-460. (Abstract in English)
24
[23] Weising, K., Nybon, H., Wolff, K. and Kahl, G. 2005. DNA fingerprinting in plants: Principles, methods and applications. CRC Press, Boca Raton, United States of America.
25
ORIGINAL_ARTICLE
Allelic diversity and association analysis for grain quality traits in exotic rice genotypes
The present research aims to study the association and allelic diversity of linked microsatellite markers to grain quality QTLs of 84 exotic rice genotypes. To this end, 9 microsatellite markers (RM540, RM539, RM587, RM527, RM216, RM467, RM3188, RM246, RM5461) were used in which a total of 61 alleles were identified with a mean of 6 alleles per locus. The polymorphism information content (PIC) varied from 0.542 (RM540) to 0.812 (RM3188) for SSR markers. Cluster analysis was performed using UPGMA method and genotypes were divided into five groups. Furthermore, based on regression analysis, for rice grain quality properties in flooding conditions as long as drought stresses, 10 alleles were identified. Of these, four alleles with gelatinization temperature, an allele with protein content under flooding conditions, and three alleles with protein content and three alleles with gelatinization temperature were related under drought stress. It should be noted that the RM216-C and RM5461-D alleles were commonly identified in several traits. The presence of common markers for traits is probably due to the consistency of chromosomal locus controlling these traits or pleiotropy. The results of this study may imply that the important identified alleles for example RM216-A for gelatinization temperature (R2=30.1 %) can be used in rice quality improvement programs.
https://www.jpmb-gabit.ir/article_36451_32943a3120dfc100a5f0023f887222ca.pdf
2018-12-01
19
26
10.22058/jpmb.2019.100495.1175
Association analysis
genetic variation
Grain physicochemical quality
Polymorphic Information Content (PIC)
Solmaz
Eimer
eimer.solmaz1372@gmail.com
1
Gonbad Kavous University, Iran
AUTHOR
hossein
sabouri
hos.sabouri@gmail.com
2
Gonbad Kavous University, Iran
LEAD_AUTHOR
Leila
Ahangar
l.ahangar63@gmail.com
3
Gonbad Kavous University, Iran
AUTHOR
Abdollatif
Gholizadeh
lgholizadeh@gmail.com
4
Gonbad Kavous University, Iran
AUTHOR
[1] Allahgholipour, M., Mohammad-Salehi, M. and Ebadi, A.A. 2005. Genetic variation and classification of cultivated rice. J. Agric. Sci, 35(4): 973-981.
1
[2] Bagheri, A., Darbandi, A. and Malboobi, M, A. 2002. Practical applications of Plant Molecular Biology. Ferdowsi Mashhad University Press, Mashhad.
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[3] Bartha, R. and Pramer, D. 1965. Features of a flask and methods of measuring the persistence and biological effects of pesticides in soil. Soil. Sci, 100: 68-70.
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[4] Bassam, B.J., Caetano-Anolles, G. and Gresshoff, P. M. 1991. Fast and sensitive silver standing of DNA in polyacrilamid gels. Analytical Biochem, 196: 80-83
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[5] Chamani Mohasses, F., Sami’zadeh, H., Rabi’ei, B. and Sohani, M. 2012. Evaluating the genetic diversity of 9 rice lines using the ISSR molecular markers. 12th Iranian Congress, 6: 1-12.
5
[6] Deshmukh, V.V. 2012. Genome-wide association mapping of drought resistance traits in rice (Oryza sativa L.). MSc thesis, Tamil Nadu Agricultural University, Tamil, The India.
6
[7] He, P., Li, S.G., Qian, Q., Ma, Y.Q. and Li, J.Z. 1999. Genetic analysis of rice grain quality. Theor. Apel. Genet, 98: 502-508.
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[8] Juliano, B.O. 1979. Amylose analysis in rice-A review. In: Proc. Workshop on Chemical Aspects of Rice Grain Quality. IRRI: Los Bafnos, Laguna, Philippines.
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[9] Lau WCP, Rafii MY, Ismail MR, Puteh A, Latif MA and Ramli A. 2015. Review of functional markers for improving cooking, eating, and the nutritional qualities of rice. Front. Plant Sci. 6:832-842.
9
[10] Liu K., Muse SV. 2005. Power Marker: an integrated analysis environment for genetic marker analysis. Bioinformatics. 21(9):2128-9
10
[11] Little, R.R., Hilder, G.B. and Dawson, E.H. 1958. Differential effect of dilute alkali on 25 varieties of milled white rice. Cereal Chem, 35: 111-126.
11
[12] Momeni, A. S. 1995. Investigating the combining ability of gene type of activity and study of correlation for important agronomic traits in different rice varieties. Master's Thesis, University of Tehran, Tehran, Iran.
12
[13] Mondini, L., Noorani, A. and Mario, A. 2009. Assessing plant genetic diversity by molecular tools. Divers, 1: 19-35.
13
[14] Musyoki, M.A., Kioko, W.F., Mathew, N.P., Daniel, A., Muriira, K.G., Wavinya, N.D., Felix, M., Chemutai, L.R., Mwenda, N.S., Kiambi, M.J. and Ngithi, N. L. 2015. Genetic diversity studies on selected rice (Oryza sativa L.) genotypes basedon amylose content and gelatinization temperature. Advances in Crop Sci. and Technol, 3(5): 1-6.
14
[15] Nevo, E. 1978. Genetic variation in natural populations. Patterns and theory. Theor. Popul. Biol, 13: 121-127.
15
[16] Nikzadeh Talebi, S., Alaami, A., Esfahani, M. and Ebadi, A.S. 2016. Evaluation of allelic abundance and communication analysis of microsatellite markers with some traits related to pre-harvest germination in rice cultivars. J. Agric. Sci. Iran, 18: 49-62.
16
[17] Palanga, K.K., Traore, K., Bimpong, K., Jamshed, M. and Mkulama, A.P. 2016. Genetic diversity studies on selected rice varieties grown in Africa based on aroma, cooking and eating quality. Afr. J. Biotechnology, 15(23): 1136-1146.
17
[18] Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A. and Allard, R.W. 1984. Ribosomal DNA sepacer-length polymorphism in barley: Mendelian inheritance, chromosomal locus, and population dynamics. In: Proc. Natl. Acad. Sci. USA, 91: 4566-4570.
18
[19] Seck, P. A., Diagne, A., Mohanty, S., & Wopereis, M. C. (2012). Crops that feed the world 7: Rice. Food security, 4(1), 7-24.
19
[20] Verma, H., Pathak, K., Rathi, S. and Sarma, S.N. 2015. Association analysis for grain quality traits in rice. Indian J. Genet, 75(4): 506-509.
20
[21] Victoria, C.L., Darshan, S.D., Toshinori, A. and Edilberto, D.R. 2007. Assessment of genetic diversity of Philippine rice cultivar carrying good quality traits using SSR markers. Breed. Sci, 57: 253-270.
21
[22] Zhang, Z., Li, M., Fang, Y., Liu, F. and Lu, Y. 2012. Diversification of the waxy gene is closely related to variations in rice eating and cooking quality. Plant Mol. Biol. Rep, 30: 462-469.
22
ORIGINAL_ARTICLE
Evaluation of salinity response through the antioxidant defense system and osmolyte accumulation in a mutant rice
In order to assess the responses of Hashemi rice genotype and its advanced mutant line under salinity stress of 100 mM Sodium chloride (NaCl) for three and six days the shoot samples were taken for biochemical analysis. This experiment was performed in split plot based on randomized complete block design with three replications. The main factor was factorial combination of saline treatment and sampling period, sub factor included genotypes. The result showed that the chlorophyll content decreased (16.3) under salt stress for the wild type, but higher amount (21.2) in the mutant was recorded. The mutant rice showed higher amount of K+ and lower of Na+ concentrations in shoots under salt stress condition. The results revealed, although the amount of H2O2 of both genotypes was significantly increased by exposure to NaCl, the effect was superior in the wild genotype (44.85). The antioxidant enzymes activity include catalase and peroxidase activity were grow up significantly in advanced mutant line. Also, the level of flavonoids and phenol content under salinity stress were enhanced dramatically in mutant line. In order to evaluate ion homeostasis under salinity stress condition the measurement of osmolytes such as proline, glycine betaine and trehalose indicated the mutant rice by rising the production (4.4, 0.81 and 87.55 respectively) of these metabolites in shoot showed the better tolerance to salinity stress. In conclusion, the observation indicated that mutation had a positive impact on ROS scavenging system and ion homeostasis mechanism and ultimately have led to salt tolerance in the mutant genotype.
https://www.jpmb-gabit.ir/article_36752_787f5bf8c0474042ad1585febfde9382.pdf
2018-12-01
27
37
10.22058/jpmb.2019.114746.1192
Enzyme Activity
Ion homeostasis
Mutation
Sodium chloride
Maryam
Forough
maryamforough88@gmail.com
1
student of Nuclear Agriculture- Plant breeding and biotechnology, Gorgan University of Agricultural Sciences and Natural Resources. Gorgan, Iran.
AUTHOR
Saeid
Navabpour
s.navabpour@yahoo.com
2
Associate Professor of Agronomy Dept. Gorgan University of Agricultural Sciences and Natural Resources, Iran
LEAD_AUTHOR
Esmaeil
Ebrahimie
ebrahimiet@gmail.com
3
Genomics Research Platform, School of Life Sciences, La Trobe University, Melbourne, Victoria 3086, Australia
AUTHOR
Ali Akbar
Ebadi
ebady_al@yahoo.com
4
Rice Research Institute of Iran (RRII), Agricultural Research Education and Extension Organization (AREEO) Rasht, Iran.
AUTHOR
Davood
Kiani
d.kiani1986@yahoo.com
5
Research assistance professor., Seed and Plant Improvement Research Department, Bushehr Agricultural and Natural Resources Research and Education Center, AREEO, Bushehr, Iran.
AUTHOR
[1] Abdallaha, M.M.S., Abdelgawadb, Z.A. and El-Bassiounya, H.M.S. 2016. Alleviation of the adverse effects of salinity stress using trehalose in two rice varieties. S. Afr. J. Bot, 103:275-282.
1
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ORIGINAL_ARTICLE
High-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)
To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) were used as explant. Leaf explants were transformed using three Agrobacterium tumefaciens strains (EHA105, GV3101, and LBA4404) harboring the binary vector pCAMBIA1304 using three different types of transformation methods as named Agro-inoculation, Agro-infection and Agro-injection. Selection of hygromycin resistant shoots was conducted on MS medium containing 3.0 mgL-1 BAP and 0.2 mgL-1 IAA, 250 mgL-1 cefotaxime and 30 mgL-1 hygromycin. Hygromycin resistant shoots were then rooted on MS medium supplemented with 250 mgL-1 cefotaxime and 15 mgL-1 hygromycin. The results indicated that A. tumefaciens strain LBA4404 was more effective in gene delivery than EHA105 and GV3101 and Agro-infection method proved to be significantly better than two other methods. The highest transformation rate was obtained with the Agrobacterium strain LBA4404 and Agro-infection method with approximately 72.80%, 84.57%, and 93.33% for N. benthamiana, Samsun and Xanthi, respectively. Histochemical GUS assay confirmed the expression of gusA gene in putatively transformed plantlets. PCR and RT-PCR analysis using gene-specific primers confirmed the integration of the gusA and hpt genes and the expression of the gusA and hpt genes, respectively. Furthermore, Southern blot analysis confirmed stable integration of the gusA gene in selected T0 transformants.
https://www.jpmb-gabit.ir/article_37011_59b96d792f5f5b36f2c784af6ba9f7b8.pdf
2018-12-01
38
50
10.22058/jpmb.2019.92266.1170
Agrobacterium tumefaciens
Direct somatic embryogenesis
regeneration
Tobacco
transformation
Reza
Heidari Japelaghi
rezaheidari59@yahoo.com
1
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tabriz University, Tabriz, Iran
AUTHOR
Raheem
Haddad
raheemhaddad@yahoo.co.uk
2
Department of Agricultural Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran
LEAD_AUTHOR
Mostafa
valizadeh
mvalizadeh@tabrizu.ac.ir
3
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tabriz University, Tabriz, Iran
AUTHOR
Ebrahim
Dorani Uliaie
uliaie@yahoo.com
4
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tabriz University, Tabriz, Iran
AUTHOR
Mokhtar
Jalali Javaran
m_jalali@modares.ac.ir
5
Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
AUTHOR
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ORIGINAL_ARTICLE
Chemical Mutagen Effect on Physiological Properties of Stevia rebaudiana Bertoni under Salt Stress
The present study was performed to evaluate the effects of different concentrations of ethyl methane sulfonate (0, 0.1, 0.2 and 0.5%) on some physiological characteristics of regenerated plants from calli of stevia at 30, 60 and 120 min under various levels of salinity stress (0, 50 and 100 mM of NaCl). This experiment was carried out based on completely randomized two-factorial designs with three replications. With respect to the result, the regenerated calli became dark and hidden in the medium under exposure time of 120 min, the length of stem regenerated calli was increased under exposure times of 30 and 60 min. Moreover, our data showed that EMS mutagenesis had a significant effect on physiological traits of regenerated stevia under salinity stress at the probability level of 1%. Consequently, the stevia mutants of M10, M11, and M19 showed the highest resistance to different levels of salinity which can be considered as potential samples for further breeding programs.
https://www.jpmb-gabit.ir/article_37232_52e210c29cb3f30c25ef6413959bd600.pdf
2018-12-01
51
60
10.22058/jpmb.2019.105902.1182
abiotic stress
EMS
Medicinal plant
Mahyar
Gerami
mahyar.gerami@yahoo.com
1
Department of Agriculture, Faculty of Biology, Sana Institute of Higher Education, Sari, Iran
LEAD_AUTHOR
Hossein
Abbaspour
hosseinabbaspour@yahoo.com
2
Department of Biology, Faculty of Agriculture, Islamic Azad University, Damghan, Iran
AUTHOR
Vali Allah
Ghasemi Omran
valioallah@yahoo.com
3
Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural sciences and Natural Resources university, Sari, Iran
AUTHOR
Hemat-Allah
Pirdashti
hematallah@yahoo.com
4
Department of Agronomy and Plant Breeding, Faculty of Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
AUTHOR
Parastoo
Majidian
parastoomajidian63@gmail.com
5
Crop and Horticultural Science Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO)
AUTHOR
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4
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[29] Zhao, M. X., Sun, H. Y., Ji, R. R., Hu, X. H., Sui, J. M., Qiao, L.X., Chen, J., Wang, J.S. 2013. In vitro mutagenesis and directed screening for salt-tolerant mutants in peanut.Euphytica, 193(1): 89-99.
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30
ORIGINAL_ARTICLE
Bacillus thuringiensis - Mediated Priming Induces Jasmonate/Ethylene and Salicylic Acid-Dependent Defense Pathways Genes in Tomato Plants
Bacillus thuringiensis Berliner as a biological control agent can play a crucial role in the integrated management of a wide range of plant pests and diseases. B. thuringiensis is expected to elicit plant defensive response through plant recognition of microbe-associated molecular patterns (MAMPs), however, there is little information on the molecular base of induced systemic resistance priming of tomatoes. Using q-RT-PCR technique, the transcription rate of the genes responsive to salicylic acid, SA (Chi9, Chi3, PR1), jasmonic acid, JA (Pin2), and of the signaling regulatory genes of jasmonate/ ethylene, JA/ ET hormones (WRKY33, ERF1, MYC2) were studied at the time of 6, 12, 24, 48, 72, and 96 hours after inoculation of tomato plants with B. thuringiensis strain IBRC-M 11096 as the promoting plant growth factor. The bacterial strain could prime tomato cultivar of Early Urbana through induction of all three hormonal signaling pathways (SA, JA, and ET) involved in the resistance to a broad range of necrotrophic as well as biotrophic pathogens. However, further transcription of WRKY33, ERF1, MYC2, and Pin2 genes in the inoculated plants, indicated that the observed priming effect was mainly based on JA/ ET signaling pathway. These promising results indicate high potential of superior isolates of B. thuringiensis in the field management of the crops.
https://www.jpmb-gabit.ir/article_37341_7c05e6ec0ea1e910e2c2ad1d1efb17ca.pdf
2018-12-01
61
69
10.22058/jpmb.2019.116294.1196
Beneficial soil microbes
Hormone signaling pathway
Induced systemic resistance
Systemic acquired resistance
Masumeh
Dezhabad
masumedejabad70@gmail.com
1
Department of Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran
AUTHOR
Hengameh
Taheri
academichngthr@gmail.com
2
Department of Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
LEAD_AUTHOR
Babak
Pakdaman Sardrood
bpakdaman@yahoo.com
3
2. Department of Plant Protection, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Khuzestan, Iran
AUTHOR
[1] Petatan-Sagahon, I., Anducho-Reyes, M.A., Silva-Rojas, H.V., Arana-Cuenca, A., Tellez-Jurado, A., Cárdenas-Álvarez, I.O. and Mercado-Flores, Y. 2011. Isolation of bacteria with antifungal activity against the phytopathogenic fungi Stenocarpella maydis and Stenocarpella macrospora. Int J Mol Sci, 12(9): 5522-5537.
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[7] Verhagen, B.W.M., Glazebrook, J., Zhu, T., Chang, H.S., Van Loon, L.C., Pieterse, C.M.J. 2004. The transcriptome of rhizobacteria-induced systemic resistance in Arabidopsis. Mol Plant Microbe Interact,17:895–908
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[11] Pieterse, C. M. J., Van Wees, S. C. M., Van Pelt, J. A., Knoester, M., Laan, R., Gerrits, H., Weisbeek, P. J., Van Loon, L. C. 1998. A novel signaling pathway controlling induced systemic resistance in Arabidopsis. Plant Cell, 10(9): 1571–1580.
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