TY - JOUR ID - 11955 TI - Primary root growth, tissue expression and co-expression analysis of a receptor kinase mutant in Arabidopsis JO - Journal of Plant Molecular Breeding JA - JPMB LA - en SN - 2322-3332 AU - Najafi zarrini, Hamid AU - Mohammad Jani Asrami, Mahsa AD - Plant Breeding Department, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran. Y1 - 2014 PY - 2014 VL - 2 IS - 2 SP - 88 EP - 95 KW - Arabidopsis KW - mutant KW - Receptor Like Kinase KW - root DO - 10.22058/jpmb.2014.11955 N2 - There is no functional annotation for the majority of the several hundreds of receptor-like kinases in plants. A direct way of inferring the function of these proteins is to study the phenotype that results from loss of function mutants such as T-DNA mutant lines. In this research a function (phenotype) to At2g37050 gene that encodes a receptor like kinase in Arabidopsis T-DNA line was assigned. This phenotype has a shorter primary root length at later stages of development. Transcription study of the gene showed some tissue specificity with more expression level in the root in comparison with other tissues. To study genes co-expressed with At2g37050,  ATTED-II web tool was used. It was found that the CLASP gene is co-expressed with At2g37050with aPearson correlation > 0.6. In kinematic analysis of the difference in root growth, the length between the root tip and the first epidermal cell with a visible root hair bulge for 8 day-old seedlings of wild type plants was 1327± 76.50 µm (n=6) and for the mutant plants, was 1109± 72.28. This parameter of the wild type and the mutant plants shows that loose of function of At2g37050 gene, reduce cell elongation in the elongation zone of root.   UR - https://www.jpmb-gabit.ir/article_11955.html L1 - https://www.jpmb-gabit.ir/article_11955_9e6b8a939716465ee4fda8550c8ffad8.pdf ER -