TY - JOUR ID - 1704 TI - Improved salt tolerance in canola (Brasica napus) plants by overexpression of Arabidopsis Na+/H+ antiporter gene AtNHX1 JO - Journal of Plant Molecular Breeding JA - JPMB LA - en SN - 2322-3332 AU - Doranie Uliaie, Ebrahim AU - Ghareyazi, Behzad AU - Farsi, Mohammad AU - Kogel, Karl-Heinz AD - Tabriz University AD - Agricultural Biotechnology Research Institute of Iran AD - Ferdowsi University of Mashhad AD - Justus Liebig University Y1 - 2012 PY - 2012 VL - 1 IS - 1 SP - 34 EP - 42 KW - Genetic Engineering KW - Na+/H+ antiporter KW - Salt tolerance KW - Canola DO - 10.22058/jpmb.2013.1704 N2 - A significant portion of the world’s cultivated land is affected by salinity that reduces crop productivity in these areas. Breeding for salt tolerance is one of the important strategies to overcome this problem. Recently, genetic engineering is becoming a promising approach to improving salt tolerance. In order to improve the yield performance of canola in saline soils, we transformed canola with Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1 which enhances the plant capacity for reducing cytosolic Na+ by transporting Na+ into the vacuole. Southern analysis of putative transgenic plants indicated that only one copy of the gene integrated into the plant genome. Overexpression of the AtNHX1 gene was shown in T1 transgenic plants. Under salinity conditions,  stem and root length and overall biomass of transgenic plants were significantly higher compared to those of nontransgenic plants. Moreover, salt treated transgenic plants contained high proline and K+, but less Na+ compared to wild type.  UR - https://www.jpmb-gabit.ir/article_1704.html L1 - https://www.jpmb-gabit.ir/article_1704_475f4597a23ae5c781f706ac7dd1a89d.pdf ER -