TY - JOUR ID - 248964 TI - Optimizing the callogenesis and determining the gamma-ray intensity in leaf explant of cut carnation standard cultivars JO - Journal of Plant Molecular Breeding JA - JPMB LA - en SN - 2322-3332 AU - Sabaghi, Hamidreza AU - Sharifi-Sirchi, Gholamreza AU - Azadi, Pejman AU - Azimi, Mohamad Hossein AD - Department of Horticultural Science, Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran AD - Department of Biotechnology Engineering, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran AD - Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran AD - Ornamental Plants Research Center (OPRC), Horticultural Sciences Research Institute (HSRI), Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran Y1 - 2020 PY - 2020 VL - 8 IS - 2 SP - 22 EP - 28 KW - callus induction KW - carnation KW - PGRs KW - In vitro mutagenesis KW - Mutation breeding DO - 10.22058/jpmb.2022.534487.1238 N2 - The present study has been designed and executed to determine the best growth-regulating compound for callus induction as well as to specify the optimum dose of gamma irradiation in carnation cultivars (Tabasco, Nobless, Cameron, Tabor, Eskimo, and Mariposa). In this experiment, an MS culture medium was used to evaluate the various levels of growth regulator concentrations including NAA in four levels (0, 0.5, 1, and 2 mgl-1), 2,4-D in five levels (0, 0.5, 1, 2, and 3 mgl-1), and BA in two levels (0.5 and 1 mgl-1). Irradiating the callus of leaf explants was carried out three weeks after cultivation at 0, 15, 25, 35, 45, and 55-gray doses-to determine the optimum dose of gamma radiation. The analysis of data and illustration of graphs were carried out via Excel software and according to the obtained results, the radiation level that killed 50% of the calluses was selected as the optimum dose for further experiments. The results have indicated that all main effects and the interaction effects regarding the characteristics of callogenesis percentage and callus volume were significant at a probability level of 1%. Means were grouped using Duncan's multiple range test, revealing that the highest level of callus induction was in Eskimo cultivar with a 73% overall mean. Overall, the results indicate that 2 mgl-1 2,4-D, 0.5 mgl-1 BA is the best regulatory compound for callogenesis in carnation cultivars. Moreover, it was found that on average, the 25-gray dose leads to suitable results in the callus explants of all cultivars.  UR - https://www.jpmb-gabit.ir/article_248964.html L1 - https://www.jpmb-gabit.ir/article_248964_51ce097bbf8bd2dd0b9a68ef6015ed41.pdf ER -