TY - JOUR ID - 249031 TI - Glomus mosseae symbiosis affects expression of stress-responsive genes in rice under water deficit conditions JO - Journal of Plant Molecular Breeding JA - JPMB LA - en SN - 2322-3332 AU - Pourfarid, Arezoo AU - Pakdin-Parizi, Ali AU - Ghorbani-Nasrabad, Reza AU - Rahimian, Heshmatollah AD - Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University AD - Genetics and Agricultural biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran AD - Department of Soil Science, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran AD - Department of plant protection, Sari University of agricultural sciences and natural resources, Sari, Iran Y1 - 2020 PY - 2020 VL - 8 IS - 2 SP - 61 EP - 72 KW - Arbuscular Mycorrhizal Fungi KW - Aquaporin KW - Expansin KW - polyamine accumulation KW - qRT-PCR DO - 10.22058/jpmb.2022.546717.1249 N2 - Arbuscular mycorrhizal fungi (AMF) symbiosis could mitigate the adverse effects of abiotic stresses in various plants. The aim of this study was to investigate the effect of AMF-inoculation on expression of several stress-responsive genes in two rice cultivars under different water conditions. The seedlings of Tarom-Hashemi and Nemat rice cultivars were transplanted in soil with or without G. mosseae spores. At the tilling stage, the AMF-inoculated (+AMF) and AMF-uninoculated (−AMF) plants were subjected to flooded and water deficit conditions (70% field capacity (FC) and 50%FC). The genes expression was evaluated by qRT-PCR and reported relative to control (flooded, -AMF) plants. The results showed lower expression of osDREB2A in +AMF plants in comparison with –AMF plants under water deficient conditions. The expression of OsPIP1;2 was significantly increased in roots of +AMF to –AMF plants. But, the expression of this gene was decreased in shoots of +AMF and –AMF plants in comparison with control plants. The stress-responsive gene transcripts, OsPIP2;3, OsGH3-8, OsLTP, OsAOS2 and OsADC1 in +AMF rice cultivars was higher than -AMF plants at both water deficit conditions. Expression of OsP5CS in +AMF and –AMF plants was increased in comparison with control plants, though, their differences was not significant. In 70%FC, OsEXP15 gene expression of +AMF and –AMF root plants was increased in comparison with control plants. However, under 50%FC the gene expression was decreased and not changed in -AMF and +AMF plants, respectively. It seems AMF induced changes in rice genes expression may enhance tolerance to water deficit conditions. UR - https://www.jpmb-gabit.ir/article_249031.html L1 - ER -