TY - JOUR ID - 27878 TI - Molecular responses of Phytophthora capsici-challenged cucumber (Cucumis sativus L.) plants as influenced by resistance inducer application JO - Journal of Plant Molecular Breeding JA - JPMB LA - en SN - 2322-3332 AU - Khosravi, Fatemeh AU - Gharanjik, Shahrokh AU - Dehestani, Ali AD - Department of Biotechnology, Shahrood University of Technology, Shahrood, Iran AD - Molecular Genetics Department, Genetics and Agricultural Biotechnology Institute of Tabrestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Y1 - 2017 PY - 2017 VL - 5 IS - 2 SP - 1 EP - 10 KW - Resistance inducer KW - Cucumber KW - potassium phosphite KW - Chitosan KW - Defense Response DO - 10.22058/jpmb.2017.69122.1142 N2 - Phytophthora species are considered as the major cause of several plant diseases resulting in huge yield losses in agricultural crops. Despite years of effort to develop Phytophthora resistance varieties, there is no reports of a resistant cucumber variety. In this study, the effect of concomitant application of potassium phosphite (KPhi) and chitosan on some physiological and molecular responses of Phytophthora capsici-challenged cucumber plants were investigated. Cucumber plants were treated with KPhi and/or Chitosan at different concentrations and were then inoculated with zoospores of P. capsici and leaf samples were collected at diļ¬€erent time courses. Results showed that Guaiacol peroxidase (GPOD) enzymatic activity surged immediately at first and second days after pathogen inoculation with a peak in plants treated with 4 gL-1 KPhi 2 days after inoculation. Compared to GPOD, the highest superoxide dismutase (SOD) activity was observed in the same treatment but later at 5 days after inoculation. It was indicated that the activity of antioxidant enzymes was greatly influenced by application of either KPhi or chitosan while their activity was not remarkably enhanced in control plants. qPCR analysis revealed that the highest increase in glutathione peroxidase (gpx) gene expression was achieved in plants concomitantly treated with 4 gL-1 KPhi and 200 mgL-1 chitosan 5 days after inoculation. The findings of this study provide novel information regarding inducing mechanisms of KPhi and chitosan which may be effective in mitigating disease severity. UR - https://www.jpmb-gabit.ir/article_27878.html L1 - https://www.jpmb-gabit.ir/article_27878_571985e61a7b33cdb7f0895254eaa7be.pdf ER -