TY - JOUR ID - 3261 TI - Assessment of genetic diversity among and within Iranian chamomile populations using semi random intron-exon splice junction (ISJ) markers JO - Journal of Plant Molecular Breeding JA - JPMB LA - en SN - 2322-3332 AU - Salehi, Keivan AU - Nazarian Firouzabadi, Farhad AD - Agronomy and plant breeding group, Faculty of Agriculture, Lorestan University Y1 - 2013 PY - 2013 VL - 1 IS - 2 SP - 40 EP - 53 KW - Iranian Chamomile KW - Matricaria chamomilla KW - Genetic diversity KW - ISJ markers KW - Polymorphism DO - 10.22058/jpmb.2013.3261 N2 - Chamomile (Matricaria chamomilla), an important medicinal plant belonging to the Asteraceae family, has a wide distribution in Iran and other parts of the world. The medicinal and pharmacological effects of chamomile are mainly associated with its essential oil content and it is widely used in food, cosmetics and pharmaceutical industries. Despite its wide geographical distribution in Iran, little is known about its molecular genetic diversity and distribution. In this study, intron-exon splice junction (ISJ) markers, including both intron-targeted (IT) and exon-targeted (ET) primers, were used to assess the genetic diversity of thirty-one chamomile populations, including 28 populations from different parts of Iran, one Hungarian population, and two of unknown origin. Twenty-six out of thirty-five primers used in the study, were reliable, producing a total number of 566 sharp and precise bands, of which 557 bands were polymorphic (98%). The average polymorphic information content (PIC) and the average marker index (MI) were calculated at 0.33 and 7.34, respectively. The average total genetic diversity (HT), average genetic diversity within population (HST) and gene differentiation coefficient (Gst) were 0.293, 0.219, and 0.251, respectively. The diversity data revealed that the Matricaria chamomilla species exhibited the closest relationship with the Tripleurospermum disciforme and Tripleurospermum sevanense species. UR - https://www.jpmb-gabit.ir/article_3261.html L1 - https://www.jpmb-gabit.ir/article_3261_ea7043f6528aedf82984caabba11e64e.pdf ER -