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Habibi, M., Malekzadeh-Shafaroudi, S., Marashi, H., Moshtaghi, N., Nasiri, M., Zibaee, S. (2014). The transient expression of coat protein of Foot and Mouth Disease Virus (FMDV) in spinach (Spinacia oleracea) using Agroinfiltration. Journal of Plant Molecular Breeding, 2(2), 18-27. doi: 10.22058/jpmb.2014.11950
Maziar Habibi; Saeid Malekzadeh-Shafaroudi; Hassan Marashi; Nasrin Moshtaghi; Mohammadreza Nasiri; Saeed Zibaee. "The transient expression of coat protein of Foot and Mouth Disease Virus (FMDV) in spinach (Spinacia oleracea) using Agroinfiltration". Journal of Plant Molecular Breeding, 2, 2, 2014, 18-27. doi: 10.22058/jpmb.2014.11950
Habibi, M., Malekzadeh-Shafaroudi, S., Marashi, H., Moshtaghi, N., Nasiri, M., Zibaee, S. (2014). 'The transient expression of coat protein of Foot and Mouth Disease Virus (FMDV) in spinach (Spinacia oleracea) using Agroinfiltration', Journal of Plant Molecular Breeding, 2(2), pp. 18-27. doi: 10.22058/jpmb.2014.11950
Habibi, M., Malekzadeh-Shafaroudi, S., Marashi, H., Moshtaghi, N., Nasiri, M., Zibaee, S. The transient expression of coat protein of Foot and Mouth Disease Virus (FMDV) in spinach (Spinacia oleracea) using Agroinfiltration. Journal of Plant Molecular Breeding, 2014; 2(2): 18-27. doi: 10.22058/jpmb.2014.11950

The transient expression of coat protein of Foot and Mouth Disease Virus (FMDV) in spinach (Spinacia oleracea) using Agroinfiltration

Article 2, Volume 2, Issue 2, Autumn 2014, Page 18-27  XML PDF (1770 K)
Document Type: Research Paper
DOI: 10.22058/jpmb.2014.11950
Authors
Maziar Habibi1; Saeid Malekzadeh-Shafaroudi1; Hassan Marashi1; Nasrin Moshtaghi1; Mohammadreza Nasiri1; Saeed Zibaee2
1Department of Biotechnology and plant breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
2Razi Vaccine and Serum Research Institute, Mashhad, Iran.
Abstract
Foot and Mouth Disease (FMD) is a very dangerous livestock disease which causes a serious loss in the production of milk and meat. Therefore, producing an effective recombinant subunit vaccine virus this disease is of great importance. Transient gene expression is a valuable tool to reach rapid and acceptable recombinant vaccine. An Agrobacterium-mediated transient gene expression assay was carried out in spinach (Spinacia oleracea) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1. The plant leaves were transformed via agroinfiltration procedure. The presence of foreign gene and its expression in transformed plants were confirmed through polymerase chain reaction (PCR), real time PCR, protein Dot blot and ELISA. The results obtained in this examination showed quite a high level of gene expression in spinach leaves, showing that transient gene expression can be applied as an effective and time-saving procedure for the production of recombinant proteins. The procedures for transformation, detection of recombinant protein and its application for molecular experiments are described in the study.
Keywords
agroinfiltration; FMDV; recombinant vaccine; spinach; VP1
Main Subjects
Molecular biology and (trans) gene expression technology in plant breeding
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