Determination of genetic uniformity in transgenic cotton plants using DNA markers (RAPD and ISSR) and SDS-PAGE

Document Type: Research Paper

Authors

1 Department of Agricultural Biotechnology, Payame Noor University, Tehran, Iran

2 Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University, Tehran, Iran

3 Agricultural Biotechnology Research Institute of Iran, Karaj, Iran

Abstract

One concern about using transgenic plants is the genetic variation that occurred from theirs tissue culture and regeneration. Molecular markers are an important element for efficient and effective determination of genetic variation. The present work was carried out to assess the genetic uniformity of transgenic cottons (Bt and chitinase lines), using RAPD, ISSR molecular markers and SDS-PAGE analysis. Similarity matrix for RAPD marker based on Nei and Li’s coefficient revealed that pairwise value between Bt and chitinase cottons and their conventional counterpart plant was 93% and 95% respectively. Also, similarity matrix for ISSR marker revealed that pair wise value between Bt and chitinase lines and their conventional counterpart was 94% and 96% respectively. Pairwise similarity coefficient among two transgenic plants and their conventional counterpart ranged from 0.94 to 0.99 SDS-protein patterns exhibited four major bands, molecular weight ranged from 10 KD to 35 KD. Similarity matrix for protein patterns revealed that pairwise value between Bt and chitinase lines and their conventional counterpart was 100%.All protein bands of three cultivars are the same and no polymorphic bands was detected. Pairwise similarity coefficient among two transgenic plants and their conventional counterpart ranged from 0.94 to 0.99. These lines could be used for more in-dept.evaluation and biosafety studies on their way to commercialization

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Main Subjects


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