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Movahedi, M., Ghasemi-Omran, V., Torabi, S. (2015). The effect of different concentrations of TDZ and BA on in vitro regeneration of Iranian cannabis (Cannabis sativa) using cotyledon and epicotyl explants. Journal of Plant Molecular Breeding, 3(2), 20-27. doi: 10.22058/jpmb.2015.15371
Mehdi Movahedi; Valiollah Ghasemi-Omran; Sepide Torabi. "The effect of different concentrations of TDZ and BA on in vitro regeneration of Iranian cannabis (Cannabis sativa) using cotyledon and epicotyl explants". Journal of Plant Molecular Breeding, 3, 2, 2015, 20-27. doi: 10.22058/jpmb.2015.15371
Movahedi, M., Ghasemi-Omran, V., Torabi, S. (2015). 'The effect of different concentrations of TDZ and BA on in vitro regeneration of Iranian cannabis (Cannabis sativa) using cotyledon and epicotyl explants', Journal of Plant Molecular Breeding, 3(2), pp. 20-27. doi: 10.22058/jpmb.2015.15371
Movahedi, M., Ghasemi-Omran, V., Torabi, S. The effect of different concentrations of TDZ and BA on in vitro regeneration of Iranian cannabis (Cannabis sativa) using cotyledon and epicotyl explants. Journal of Plant Molecular Breeding, 2015; 3(2): 20-27. doi: 10.22058/jpmb.2015.15371

The effect of different concentrations of TDZ and BA on in vitro regeneration of Iranian cannabis (Cannabis sativa) using cotyledon and epicotyl explants

Article 3, Volume 3, Issue 2, Summer and Autumn 2015, Page 20-27  XML PDF (700 K)
Document Type: Research Paper
DOI: 10.22058/jpmb.2015.15371
Authors
Mehdi Movahedi1; Valiollah Ghasemi-Omran 2; Sepide Torabi3
1Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Genetics and Agricaltural Biotechnology Institute of Tabarestan, Sari Agricaltural Sciences and Natural Resources University, Sari, Iran
3Department of Plant Breeding and Biotechnology, Science and Research, Islamic Azad University, Tehran, Iran
Abstract
The present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (PGRs) combinations under in vitro conditions. The cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in MS medium containing BA (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and TDZ (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) either alone or in combination with 0.5 mg-1 IBA. The response of cannabis explants to PGRs treatments was much different from those observed in most of plant species. That is, callus formation had priority over direct regeneration in most of the PGRs treatments. Comparing the two explants, cotyledon had higher callus formation frequency and the largest callus volume was obtained for this explant in MS medium supplemented with 3 mg-1 TDZ + 0.5 mg-1 IBA. The highest callus fresh weight (3.15 gr) was obtained for cotyledon explant treated with 2 mg-1 TDZ+ 0.5 mg-1 IBA. In shoot formation step, the highest rate of shoot regeneration was achieved in the calli produced from epicotyl explant treated with 2 mg-1 BA + 0.5 mg-1 IBA; and the highest length of regenerated shoots (1.23 cm) was observed in 2 mg-1 BA + 0.5 mg-1 IBA treatment. In general, cotyledon was the best explant and TDZ in combination with IBA was the best treatment for callus formation. Epicotyl explant also showed better regeneration compared to cotyledon.
Keywords
Cannabis; callus; Plant growth regulators; Regeneration
Main Subjects
Micropropagation & plant breeding
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