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Modareskia, M., Darvishzadeh, R., Hassani, A., Kholghi, M. (2012). Molecular diversity within and between Ajowan (Carum copticum L.) populations based on inter simple sequence repeat (ISSR) markers. Journal of Plant Molecular Breeding, 1(1), 51-62. doi: 10.22058/jpmb.2013.1706
Mahdieh Modareskia; Reza Darvishzadeh; Abbas Hassani; Maryam Kholghi. "Molecular diversity within and between Ajowan (Carum copticum L.) populations based on inter simple sequence repeat (ISSR) markers". Journal of Plant Molecular Breeding, 1, 1, 2012, 51-62. doi: 10.22058/jpmb.2013.1706
Modareskia, M., Darvishzadeh, R., Hassani, A., Kholghi, M. (2012). 'Molecular diversity within and between Ajowan (Carum copticum L.) populations based on inter simple sequence repeat (ISSR) markers', Journal of Plant Molecular Breeding, 1(1), pp. 51-62. doi: 10.22058/jpmb.2013.1706
Modareskia, M., Darvishzadeh, R., Hassani, A., Kholghi, M. Molecular diversity within and between Ajowan (Carum copticum L.) populations based on inter simple sequence repeat (ISSR) markers. Journal of Plant Molecular Breeding, 2012; 1(1): 51-62. doi: 10.22058/jpmb.2013.1706

Molecular diversity within and between Ajowan (Carum copticum L.) populations based on inter simple sequence repeat (ISSR) markers

Article 7, Volume 1, Issue 1, Autumn 2012, Page 51-62  XML PDF (421 K)
Document Type: Research Paper
DOI: 10.22058/jpmb.2013.1706
Authors
Mahdieh Modareskia; Reza Darvishzadeh; Abbas Hassani; Maryam Kholghi
Urmia University
Abstract
Study of genetic relationships is a prerequisite for plant breeding activities as well as for conservation of genetic resources. In the present study, genetic diversity among and within 15 Iranian native Ajowan(Carum copticum L.) populations were determined using inter simple sequence repeat (ISSR) markers. Twelve selected primers produced 153 discernible bands, with 93 (60.78%) being polymorphic, indicating considerable genetic diversity at the population level. Number of polymorphic bands per primer ranged from 4 to 11 with an average of 7.75. The largest percentage of polymorphic loci was 78.57% for primer UBC818. Based on an un-weighted pair-group method using arithmetic average (UPGMA) clustering algorithm, four distinct groups were established. Results of the principal coordinate analysis (PCoA) corresponded to those obtained through cluster analysis. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly within populations (61%), but variance among populations was only 39%. This study has detected significant genetic differentiation among C. copticum populations. The current study confirmed the importance of molecular studies in detecting genetic variation among genotypes in selecting diverse parents to carry out crossing program successfully.
Keywords
Carum copticum; Cluster analysis; Genetic diversity; molecular markers
Main Subjects
Molecular markers & plant breeding
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