Interaction of four important factors affecting callogenesis and organogenesis of Artemisia absinthium

Document Type : Research Paper

Authors

1 Biotechnology, Engineering faculty, Immam Khomeini University

2 Agricultural biotechnology, engineering faculty, Immam Khomeini University

Abstract

Medicinal plants are becoming the main source of important therapeutic aid for alleviating human ailments. The present study was initiated to study the effect of growth regulators, carbon source and explants either in combination or singly on the callogenesis and organogenesis of Artemisia absinthuim.Various kinds of calli were obtained from different explants in the MS medium containing different concentrations of NAA, BAP and carbon sources. In the investigation of four factors, the best callogenesis was observed at 2 mg/L BAP + sucrose (30g/L) using root explants, 0.5 mg/L BAP + 0.5 mg/L NAA + sucrose (30 g/L) using root explants and 0.1 NAA + 2 mg/L BAP + sucrose (30 g/L) using leaf explants. The maximum number of shoots was induced at 0.5 mg/L BAP + 0.1 mg/L NAA + (30 g/L) glucose using leaf explants (2.167±1.484), followed by 0.1 mg/L NAA + 2 mg/L BAP + sucrose (30 g/L) (1.993 ± 0.54). However, the treatments containing 30 g/L glucose generally did not show suitable responses to induce shoots. The longest shoots were observed in the medium containing 0.5 mg/L BAP + 0.1 mg/L NAA + 30 g/L sucrose with leaf explants (0.943±0.345 cm). The maximum number of induced roots was obtained in treatments containing 2 mg/L NAA (in the absence of BAP) + 30 g/L glucose using leaf explants, while the longest induced roots was obtained in the treatment containing 0.1 mg/L NAA (in the absence of BAP) + 30 g/L glucose with leaf explants.

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