Evaluation the effect of senescence on the mineral remobilization in two bread wheat cultivars

Document Type : Research Paper

Authors

Department of plant breeding and biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

The main source of protein and micronutrients in wheat grains is the flag leaf and to a lesser extent the lower leaves. As healthy leaves reach the final stage of growth, senescence, they remobilize the nutrients necessary before tissue destruction and death. This experiment was carried out in Golestan province, and the Wheat cultivars studied were included Euclide and Antonius. Sampling was carried out from flag leaf, other leaves, stem, and grain at 7 stages, Anthesis, 7, 11, 15, 19, 23, and 27 Day After Anthesis (DAA). The total chlorophyll content in the Antonius cultivar was higher in both flag leaf and other leaves than Euclide cultivar. The expression of TaNAM-B1 and TaSAG12 genes, which have been identified as signaling genes for senescence in wheat, showed results consistent with the results of chlorophyll content in leaves. Increased expression of both genes after anthesis was observed earlier in Euclide cultivar than the Antonius cultivar and had higher expression in most stages. In light of the results, the change in concentrations of Cu, Zn, and Fe in the Euclid cultivar was more in all organs than in Antonius one. Also, given the importance of minerals in the food basket, it can be noted that Euclid cultivar, in which leaf senescence begins earlier and more minerals are stored, can produce grains with higher nutritional value than Antonius cultivar.

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[1] Ehdaie, B., Alloush, G.A., Madore, M.A. and Waines, J.G. 2006. Genotypic variation for stem reserves and mobilization in wheat: II. Postanthesis changes in internode water-soluble carbohydrates. Crop Sci, 46:2093–2103.
[2] Borrill, P., Connorton, J.M., Balk, J., Miller, A.J., Sanders, D. and Uauy, C. 2014. Biofortification of wheat grain with iron and zinc: integrating novel genomic resources and knowledge from model crops. Front Plant Sc, 5:53.
[3] Waters, B.M., Uauy, C., Dubcovsky, J., and Grusak, M.A. 2009. Wheat (Triticum aestivum) NAM proteins regulate the translocation of iron, zinc, and nitrogen compounds from vegetative tissues to grain. J. Exp. Bot. 60:4263–4274. 
[4] Veneklaas, E.J., Lambers, H., Bragg, J., Finnegan, P.M., Lovelock, C.E., Plaxton, W.C., Price, C.A., Scheible, W., Shane, M.W. and White, P.J. 2012. Opportunities for improving phosphorus-use efficiency in crop plants. New Phytol, 195:306–320.
[5] Uauy, C., Distelfeld, A., Fahima, T., Blechl, A. and Dubcovsky, J. 2006. A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science, 314: 1298-1301.
[6] Quirino, B.F., Noh, Y.S., Himelblau, E. and Amasino, R.M. 2000. Molecular aspects of leaf senescence. Trends Plant Sci. 5:278–282.
[7] Buchanan-Wollaston, V., Earl, S., Harrison, E., Mathas, E., Navabpour, S., Page, T.and Pink, D. 2003. The molecular analysis of leaf senescence—A genomics approach. Plant Biotechnol. J, 1:3–22.
[8] Guo, Y. and Gan, S.S. 2014. Translational researches on leaf senescence for enhancing plant productivity and quality. J. Exp. Bot, 65:3901–3913.
[9] Gan, S. and Amasino, R.M. 1997. Making Sense of Senescence (Molecular Genetic Regulation and Manipulation of Leaf Senescence). Plant Physiol. 1997, 113:313–319.
[10] Milla, R., Castro-Díez, P., Maestro-Martínez, M. and Montserrat-Martí, G. 2005. Relationships between phenology and the remobilization of nitrogen, phosphorus and potassium in branches of eight Mediterranea never greens. New Phytol. 168, 167–178. doi: 10.1111/j.1469-8137.2005.01477.x.
[11] Granett, T. and Graham, R. 2005. Distribution and Remobilization of Iron and Copper in Wheat. Annals of Botany 95: 817–826.
[12] Kutman, U.B., Kutman, B.Y., Ceylan, Y., Ova, E A. and Cakmak, I. 2012. Contributions of root uptake and remobilization to grain zinc accumulation in wheat depending on post-anthesis zinc availability and nitrogen nutrition. Plant Soil 361, 177–187. doi: 10.1007/s11104-012-1300-x.
[13] Hegelund, J.N., Pedas, P., Husted, S., Schiller, M. and Schjoerring, J. K. 2012. Zinc fluxes into developing barley grains: use of stable Zn isotopes to separate root uptake from remobilization in plants with contrasting Zn status. Plant Soil. 361:241–250. doi: 10.1007/s11104-012-1272-x.
[14] Erenoglu, E.B., Kutman, U.B., Ceylan, Y., Yildiz, B., Cakmak, I. 2011. Improved nitrogen nutrition enhances root uptake, root-to-shoot translocation and remobilization of zinc (65Zn) in wheat. New Phytol, 189:438–448.
[15] Shi, M., Ye, X., Yan, Y., Howit, C., Bellgard, M. and Ma, W. 2011. Gene networks in the synthesis and deposition of protein polymers during grain development of wheat. Functional & Integrative Genomics, 11:23–35.
[16] Chen, X.Y., Song, G.Q., Zhang, S.J., Li, Y.L., Gao, J., Shahidul, I., Ma, W.J., Li, G.Y. and JI, W.Q. 2017. The allelic distribution and variation analysis of the NAM-B1 gene in Chinese wheat cultivars, Journal of Integrative Agriculture, 16(6): 1294-1303.
[17] Cakmak, I., Torun, A., Millet, E., Feldman, M., Fahima, T., Korol, A., Nevo, E., Braun, H.J. and Ozkan, H. 2004. Triticum dicoccoides an important genetic resource for increasing zinc and iron concentration in modern cultivated wheat. Soil Science and Plant Nutrition, 50:1047–1054.
[18] Mae, T. 2004. Leaf senescence and nitrogen metabolism. In: Noodén L D, ed., Plant Cell Death Processes. Elsevier Academic Press, San Diego, CA, 157–168.
[19] Kade, M., Barneix, A.J., Olmos, S. and Dubcovsky, J. 2005. Nitrogen uptake and remobilization in tetraploid ‘Langdon’ durum wheat and a recombinant substitution line with the high grain protein gene Gpc-B1. Plant Breeding, 124:343–349.
[20] Murray, C.J.L. and Lopez, A.D. 2013. Measuring the global burden of disease. N. Engl. J. Med, 369:448–457. doi: 10.1056/NEJMra1201534.
[21] Porra, R.J., Thompson, W.A. and Kriedmann, P.E. 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta, 975: 384-394.
[22] Chen, D.Q., S.W. Wang, B.L. Xiong, B.B. Cao and X.P. Deng, 2015. Carbon/Nitrogen imbalance associated with drought-induced leaf senescence in Sorghum bicolor. PloS One. 10 (8): e0137026.
[23] Pfaffl, M.W. 2001 A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Research, 29:55 pp.
[24] Rengel, Z. and Romheld, V. 2000. Root exudation and Fe uptake and transport in wheat genotypes differing in tolerance to Zn deficiency, Plant and Soil, 222: 25-34.
[25] Ren, G., Zhou, Q., Wu, S., Zhang, Y., Zhang, L., Huang, J., Sun, Z. and Kuai, B. 2010. Reverse genetic identification of CRN1 and its distinctive role in chlorophyll degradation in Arabidopsis. J. Integr. Plant Biol. 52:496–504.
[26] Sakuraba, Y., Schelbert, S., Park, S.Y., Han, S.H., Lee, B.D., Andres, C.B., Kessler, F., Hortensteiner, S. and Paek, N.C. 2012. STAY-GREEN and chlorophyll catabolic enzymes interact at light-harvesting complex II for chlorophyll detoxification during leaf senescence in Arabidopsis. Plant Cell, 24:507–518.
[27] Christiansen, M.W. and Gregersen, P.L. 2014. Members of the barley NAC transcription factor gene family show differential co-regulation with senescence-associated genes during senescence of flag leaves. Journal of experimental Botany, 65:4009–4022.
[28] Yang, J. and Zhang, J. 2006. Grain filling of cereals under soil drying. New Phytologist. 169(2): 223-236.
[29] Bagherikia, S., Pahlevani, M.H., Yamchi, A., Zenalinezhad, K. and Mostafaie, A. 2018. Remobilization of stem soluble carbohydrates in bread wheat (Triticum aestivum L.) under terminal drought stress. Journal of Plant Process and Function, 7(24): 53-72. (In Persian).
[30] Zhao, Y., Chan, Z., Gao, J., Xing, L., Cao, M., Yu, C., Hu, Y., You, J., Shi, H. and Zhu, Y. 2016. ABA receptor PYL9 promotes drought resistance and leaf senescence. Proc. Natl. Acad. Sci. USA, 113:1949–1954.
[31] Podzimska-Sroka, D., O’Shea, C., Gregersen, P.L. and Skriver, K. 2015. NAC Transcription Factors in Senescence: From Molecular Structure to Function in Crops. Plants, 4:412–448.
[32] James, M., Poret, M., Masclaux-Daubresse, C., Marmagne, A., Coquet, L., Jouenne, T. and Etienne, P. 2018. SAG12, a Major Cysteine Protease Involved in Nitrogen Allocation during Senescence for Seed Production in Arabidopsis thaliana. Plant and Cell Physiology, doi:10.1093/pcp/pcy125.
[33] Desclos-Théveniau, M., Coquet, L., Jouenne, T., and Etienne, P. 2015. Proteomic analysis of residual proteins in blades and petioles of fallen leaves of Brassica napus. Plant Biol. 17:408–418. doi: 10.1111/plb.12241.
[34] James, M., Masclaux-Daubresse, C., Marmagne, A., Azzopardi, M., Laîné, P., Goux, D., Etienne, P. and Trouverie, J. 2019. A New Role for SAG12 Cysteine Protease in Roots of Arabidopsis thaliana. Front. Plant Sci, 9:1998. doi: 10.3389/fpls.2018.01998.
[35] Checovich, M.L., Galatro, A., Moriconi, J.I., Simontacchi, M., Dubcovsky, J. and Santa-María, G.E. 2016. The stay-green phenotype of TaNAM-RNAi wheat plants is associated with maintenance of chloroplast structure and high enzymatic antioxidant activity. Plant Physiology and Biochemistry, 104: 257–265.
[36] Wang, B., Wei, J., Song, N., Wang, N., Zhao, J. and Kang, Z. 2018. A novel wheat NAC transcription factor, Ta NAC30, negatively regulates resistance of wheat to stripe rust. J. Integr. Plant Biol, 60:432–443.
[37] Masclaux-Daubresse, C., Reisdorf-Cren, M. and Orsel, M. 2008. Leaf nitrogen remobilisation for plant development and grain filling. Plant Biology 10, 23–36.