Expression analysis of SiSOD gene family during Sesamum indicum L. seed germination under various abiotic stresses

Document Type : Research Paper


Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), Sari, Iran


Sesame (Sesamum indicum L.) seed is a rich source of oil and protein, which could be used for cooking or primary source for some industrial applications. Seed germination is the most fundamental stage of a plant’s life cycle, which is significantly influenced by various abiotic stresses. As a first report, the study attempted to evaluate the effect of environmental factors (i.e., low, optimum and high temperatures (T), water potential (ψ) and salinity) on eight superoxide dismutase (SOD) gene expressions (two Mn-SOD, two Cu/Zn-SOD and four Fe-SOD) during sesame germination. Results showed that all studied treatments remarkably influenced germination characteristics of sesame (P ≤ 0.05). In general, the negative impact of each stress on sesame germination could be ranked as ψ > salt stress > high T > low T, indicating that the germination was more influenced by ψ than salt stress and T. There was a strong association between the decrease in germination parameters (relative to the optimal T) and the decrease in SiSOD expression under various stresses. Our results discovered that the SiSODs expression patterns were stress-specific. However, when subjected to the same stress, the majority of SiSOD genes displayed similar expression patterns. The findings of this study could lead to a better understanding of SOD's role in other plants and the mechanisms involved in plants' stress responses, especially during their early stages of development. 


Main Subjects

[1]   Abdellaoui, R., Boughalleb, F., Zayoud, D., Neffati, M., and Bakhshandeh, E. 2019. Quantification of Retama raetam seed germination response to temperature and water potential using hydrothermal time concept. Environmental and Experimental Botany, 157: 211-216.
[2]   Alvarado, V. and Bradford, K. 2002. A hydrothermal time model explains the cardinal temperatures for seed germination. Plant, Cell & Environment, 25(8): 1061-1069.
[3]   Apel, K. and Hirt, H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol., 55: 373-399.
[4]   Bakhshandeh, E., Atashi, S., Hafeznia, M., Pirdashti, H., and Teixeira da Silva, J.A. 2015. Hydrothermal time analysis of watermelon (Citrullus vulgaris cv.‘Crimson sweet’) seed germination. Acta Physiologiae Plantarum, 37(1): 1738.
[5]   Bakhshandeh, E., Gholamhosseini, M., Yaghoubian, Y., and Pirdashti, H. 2020. Plant growth promoting microorganisms can improve germination, seedling growth and potassium uptake of soybean under drought and salt stress. Plant Growth Regulation, 90(1): 123-136.
[6]   Bakhshandeh, E., Jamali, M., Afshoon, E., and Gholamhossieni, M. 2017. Using hydrothermal time concept to describe sesame (Sesamum indicum L.) seed germination response to temperature and water potential. Acta Physiologiae Plantarum, 39(11): 250.
[7]   Bayanati, M., Tehranifar, A., Razavi, K., Nemati, S., Lohrasebi, T., and Ahmadi, N. 2019. Expression patterns analysis of SOD genes in responses to ethylene-induced oxidative stress in rose (Rosa hybrida) during flower development. South African Journal of Botany, 127: 265-270.
[8]   Bewley, J.D., Bradford, K., and Hilhorst, H. 2013. Seeds: physiology of development, germination and dormancy. 3rd edn. Springer, New York, p 407.
[9]   Blokhina, O., Virolainen, E., and Fagerstedt, K.V. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of botany, 91(2): 179-194.
[10] Gill, S.S. and Tuteja, N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant physiology and biochemistry, 48(12): 909-930.
[11] Hashemipetroudi, S.H., Nematzadeh, G., Ahmadian, G., Yamchi, A., and Kuhlmann, M. 2018. Assessment of DNA contamination in RNA samples based on ribosomal DNA. JoVE (Journal of Visualized Experiments), (131): e55451.
[12] Hosseini Sanehkoori, F., Pirdashti, H., and Bakhshandeh, E. 2021. Quantifying water stress and temperature effects on camelina (Camelina sativa L.) seed germination.
[13] Hutchins, J.R. 2019. Genomic Databases.
[14] Institute, S. 2014. SAS 9.4 Output delivery system: User's guide. SAS institute.
[15] Jiang, W., Yang, L., He, Y., Zhang, H., Li, W., Chen, H., Ma, D., and Yin, J. 2019. Genome-wide identification and transcriptional expression analysis of superoxide dismutase (SOD) family in wheat (Triticum aestivum). PeerJ, 7: e8062.
[16] Klepikova, A.V., Kasianov, A.S., Gerasimov, E.S., Logacheva, M.D., and Penin, A.A. 2016. A high resolution map of the Arabidopsis thaliana developmental transcriptome based on RNA‐seq profiling. The Plant Journal, 88(6): 1058-1070.
[17] Luan, Z., Xiao, M., Zhou, D., Zhang, H., Tian, Y., Wu, Y., Guan, B., and Song, Y. 2014. Effects of salinity, temperature, and polyethylene glycol on the seed germination of sunflower (Helianthus annuus L.). The Scientific World Journal, 2014.
[18] Michel, B.E. and Radcliffe, D. 1995. A computer program relating solute potential to solution composition for five solutes. Agronomy Journal, 87(1): 126-130.
[19] Najeeb, U., Mirza, M., Jilani, G., Mubashir, A., and Zhou, W. 2012. Sesame, in Technological Innovations in Major World Oil Crops, Volume 1, Gupta, S.K., Editor. Springer. p. 131-145.
[20] Parmoon, G., Moosavi, S.A., and Siadat, S.A. 2018. How salinity stress influences the thermal time requirements of seed germination in Silybum marianum and Calendula officinalis. Acta physiologiae plantarum, 40(9): 175.
[21] Pospíšil, P., Prasad, A., and Rác, M. 2019. Mechanism of the formation of electronically excited species by oxidative metabolic processes: role of reactive oxygen species. Biomolecules, 9(7): 258.
[22] Rehman, S., Rashid, A., Manzoor, M.A., Li, L., Sun, W., Riaz, M.W., Li, D., and Zhuge, Q. 2022. Genome-Wide Evolution and Comparative Analysis of Superoxide Dismutase Gene Family in Cucurbitaceae and Expression Analysis of Lagenaria siceraria Under Multiple Abiotic Stresses. Frontiers in genetics, 12: 784878.
[23] Rowse, H. and Finch‐Savage, W. 2003. Hydrothermal threshold models can describe the germination response of carrot (Daucus carota) and onion (Allium cepa) seed populations across both sub‐and supra‐optimal temperatures. New Phytologist, 158(1): 101-108.
[24] Sanehkoori, F.H., Pirdashti, H., and Bakhshandeh, E. 2021. Quantifying water stress and temperature effects on camelina (Camelina sativa L.) seed germination. Environmental and Experimental Botany, 186: 104450.
[25] Schmittgen, T.D. and Livak, K.J. 2008. Analyzing real-time PCR data by the comparative CT method. Nature protocols, 3(6): 1101-1108.
[26] Seal, C.E., Barwell, L.J., Flowers, T.J., Wade, E.M., and Pritchard, H.W. 2018. Seed germination niche of the halophyte Suaeda maritima to combined salinity and temperature is characterised by a halothermal time model. Environmental and Experimental Botany, 155: 177-184.
[27] Shakeri, S., Kazemitabar, S.K., and Hashemi, S.H. 2015. Study of Reference Genes in Sesame Leaves under Salt Stress by Real-Time PCR Method. Crop Biotechnology, 4(8): 1-10.
[28] Sharma, S.S. and Dietz, K.-J. 2009. The relationship between metal toxicity and cellular redox imbalance. Trends in plant science, 14(1): 43-50.
[29] Soltani, A. and Maddah, V. 2010. Simple applied programs for education and research in agronomy. Iranian Scientific Society of Agroecol. Publ.
[30] Su, W., Raza, A., Gao, A., Jia, Z., Zhang, Y., Hussain, M.A., Mehmood, S.S., Cheng, Y., Lv, Y., and Zou, X. 2021. Genome-wide analysis and expression profile of superoxide dismutase (SOD) gene family in rapeseed (Brassica napus L.) under different hormones and abiotic stress conditions. Antioxidants, 10(8): 1182.
[31] Tilaki, G.A.D., Behtari, B., and Behtari, B. 2009. Effect of salt and water stress on the germination of alfalfa (Medicago sativa L.) seed. Поволжский экологический журнал, (2): 158-164.
[32] Tunde-Akintunde, T. and Akintunde, B. 2004. Some physical properties of sesame seed. Biosystems Engineering, 88(1): 127-129.
[33] Vahabinia, F., Pirdashti, H., and Bakhshandeh, E. 2019. Environmental factors’ effect on seed germination and seedling growth of chicory (Cichorium intybus L.) as an important medicinal plant. Acta physiologiae plantarum, 41(2): 27.
[34] Valiki, S.R.H., Ghanbari, S., Golmohammadzadeh, S., and Kiasari, K.R. 2015. Effect of different plant density on growth and yield of three cultivars of sesame (Sesamum indicum L.). in Biological Forum. Research Trend.
[35] Yadav, S., Gill, S.S., Passricha, N., Gill, R., Badhwar, P., Anjum, N.A., Francisco, J.-B.J., and Tuteja, N. 2019. Genome-wide analysis and transcriptional expression pattern-assessment of superoxide dismutase (SOD) in rice and Arabidopsis under abiotic stresses. Plant gene, 17: 100165.
[36] Zhang, X., Zhang, L., Chen, Y., Wang, S., Fang, Y., Zhang, X., Wu, Y., and Xue, D. 2021. Genome-wide identification of the SOD gene family and expression analysis under drought and salt stress in barley. Plant Growth Regulation, 94(1): 49-60.