Chickpea (Cicer arietinum L.) genetic diversity detected by Inter Simple Sequence Repeat markers

Document Type : Research Paper

Authors

1 Department of Biotechnology and Plant Breeding, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Genetics and Agricaltural Biotechnology Institute of Tabarestan, Sari Agricaltural Sciences and Natural Resources University, Sari, Iran

3 Department of Plant Breeding, Sari Agricultural Science and Natural Resources University, Sari, Iran

Abstract

Genetic relationships among twenty-three Chickpea genotypes include seven Chickpea cultivars, and 16 wild genotypes were analysed by 10 ISSR markers. On average, 11 bands per primer were observed, and 83.4 % polymorphic bands were shared in ISSR analysis. The results of clustering demonstrated the efficiency of ISSR markers (particularly ISSR1 (UBC-834) and ISSR8 (UBC-864)) for reporting appropriate parents for breeding programs to biotic and abiotic stresses. Principle Coordinate Analysis (PCoA) clustered 23 Chickpea genotypes in two groups by which six cultivars separated in one group. Genetic data extracted from ISSR analysis are practical in Chickpea to choose parental sources for inbreeding programs.

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