Document Type: Research Paper
Seed and Plant improvement Research Department, Lorestan Agricultural and Natural Resources Research and Education Center, Khorramabad. Iran
Associate Professor, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
Professor, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
Agricultural Biotechnology Research Institute of Iran
Department of Plant Breeding, Yadegar- E- Imam Khomeini (RAH) of Shahre-Rey Branch, Islamic, Azad University, Tehran, Iran
Department of Agronomy and Plant Breeding, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
In order to identify yield and yield component QTLs under control and salt-stress conditions, a population of 254 recombinant inbred lines (RILs), derived from a cross between two bread wheat cultivars, (Roshan / Sabalan), was assessed. Parents and 254 recombinant inbred lines (RILs) were evaluated in an alpha-lattice design with two replications in two control and saline environments of Yazd in 2011-2012 cropping season. Yield and yield-related traits were evaluated at harvest time. The genotyping was carried out using SSR and DArT markers. A, B and D genomes were covered by 411.8, 620.4 and 67.5 cM, respectively. Also, a total of 48 QTLs were detected on 11 chromosomes for grain yield, biological yield, harvest index, thousand-grain weight, grain number per spike, spike weight and spikelet number per spike. Roshan (salt tolerance) alleles were associated with an increase yield under saline conditions. SSR markers including gwm146, gwm577, gwm249 (on chromosomes 2A and 7B) were tightly associated with different QTLs. The major effect QTLs were located on chromosomes 1A and 7B for grain yield, harvest index and spike weight, which were explained 10.2%, 12.98% and 29 % of the total phenotypic variance, respectively. These QTLs and markers could be suitable for marker-assisted selection and gene stacking techniques. Moreover, co-located QTLs were detected on chromosome 2B for evaluated traits.