Chemical mutagen effect on physiological properties of Stevia rebaudiana Bertoni under salt stress

Document Type: Research Paper


1 Department of Agriculture, Faculty of Biology, Sana Institute of Higher Education, Sari, Iran

2 Department of Biology, Faculty of Agriculture, Islamic Azad University, Damghan, Iran

3 Department of Agronomy & Plant Breeding, Faculty of Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari University of Agricultural Sciences and Natural Resources, Sari, Iran

4 seed and plant improvement research department, mazandaran agricultural and natural resources research and education center



The use of biotechnology techniques such as cellular, organ, and tissue culture as well as genetic engineering strategies could be the effective tools to increase yield of medicinal plants. The present study was performed based on completely randomized two-factorial designs with three replications to study the effects of ethyl methane sulfonate (EMS) on some of physiological characteristics of regenerated plants from calli of stevia under salinity stress. In this experiment, the calli obtained from leaves of stevia explants were exposed to different concentrations of EMS (0.1, 0.2 and 0.5%) at 30, 60 and 120 s. The resultant data indicated that different doses of EMS had an obvious influence on regenerated calli as well as the length of stem produced under 30 and 60 s of exposure time, while the regenerated calli became dark and hidden in the medium under 120 min of exposure time. Moreover, The results showed that EMS mutagenesis had a significant effect on physiological traits of regenerated stevia under salinity stress at 1% level. The study of physiological traits indicated that the highest resistance to different levels of salinity was attributed to M6, M10, M19 and M18 mutants which can be considered as potential samples for further breeding programs.