Transcript analysis of telomerase enzyme gene in sunflower infected by sclerotinia stem rot disease

Document Type : Research Paper

Authors

1 Department of Biology, Urmia Branch, Islamic Azad University, Urmia, Iran.

2 Department of Biology, Urmia branch, Islamic Azad University, Urmia, Iran

3 Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, Urmia University, Urmia, Iran.

Abstract

Sclerotinia stem rot disease caused by Sclerotinia sclerotiorum is one of the most important diseases of sunflower. Telomeres are nucleoprotein structures at the ends of chromosomes that are essential for maintaining the integrity of the genome. The aim of this study was to determine the alteration of telomerase enzyme gene (tert) expression under fungal infection stress. The expression of tert gene in both susceptible (SDR19) and resistant (LC1064-C) genotypes of sunflower was evaluated by qRT-PCR after infection with A37 isolate of S. sclerotiorum. The results showed significant and drastic decreased levels of tert expression in both susceptible and resistant genotypes of sunflower, immediately after fungal infection. This depletion followed by mild variation when infection continued for more hours, which was more constant in resistant line, compared with the susceptible one. In conclusion, the expression of tertgene in sunflower is downregulated in response to Sclerotinia rot disease.

Keywords


 [1]     Rauf S, Sadaqat HA, Khan IA, Ahmed R. 2009. Genetic analysis of leaf hydraulics in sunflower (Helianthus annuus L.) under drought stress. Plant Soil Environ, 55: 62-69.
[2]     Seiler G, Qi L, Marek L. 2017. Utilization of sunflower crop wild relatives for cultivated sunflower improvement. Crop Science, 57: 1-19.
[3]     Rauf S, Jamil N, Tariq SA, et al. 2017. Progress in modification of sunflower oil to expand its industrial value. Science of Food and Agriculture, 97: 1997-2006.
[4]     Dimitrijević A, Imerovski I, Miladinović D, Cvejić S, Jocić S, Zeremski T, Sakač Z. 2017. Oleic acid variation and marker-assisted detection of Pervenets mutation in high- and low-oleic. Crop Breeding and Applied Biotechnology, 17: 235-241.
[5]     Kane NC, Burke JM, Marek L, Seiler G, Vear F, Baute G, Knapp SJ, Vincourt P, Rieseberg LH. 2013. Sunflower genetic, genomic and ecological resources. Molecular Ecology Resources, 13: 10-20.
[6]     Garg H, Li H, Sivasithamparam K, Kuo J, Barbetti MJ. 2010. The infection processes of Sclerotinia sclerotiorum in cotyledon tissue of a resistant and a susceptible genotype of Brassica napus. Annals of Botany, 106: 897–908.
[7]     Boland GJ, Hall R. 1994. Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology, 16: 93-108.
[8]     Prats E, Galindo JC, Bazzalo ME, León A, Macías FA, Rubiales D, Jorrín JV. 2007. Antifungal activity of a new phenolic compound from capitulum of a head rot-resistant sunflower genotype. Chem Ecol, 33: 2245-2253.
[9]     Grover A, Gowthaman R. 2003. Strategies for development of fungus-resistant transgenic plants. Current Science, 84: 330-340.
[10]  Greider C, Blackburn E. 1985. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell, 43: 405-413.
[11]  Palm W, Lange TD. 2008. How shelterin protects mammalian telomeres. Annual Review of Genetics, 42: 301-334.
[12]  Ren S, Mandadi KK, Boedeker AL, Rathore KS, McKnight TD. 2007. Regulation of telomerase in Arabidopsis by BT2, an Apparent Target of Telomerase Activator1. The Plant Cell, 19: 23-31.
[13]  Aubert G, Lansdorp P. 2008. Telomeres and aging. Physiological Reviews, 88: 557-579.
[14]  Shlush LI, Skorecki KL, Itzkovitz S, Yehezkel S, Segev Y, Shachar H, Berkovitz R, Adir Y, Vulto I, Lansdorp PM, Selig S. 2011. Telomere elongation followed by telomere length reduction, in leukocytes from divers exposed to intense oxidative stress – Implications for tissue and organismal aging. Mechanisms of Ageing and Development, 132: 123-130.
[15]  Fajkus J, Kovarík A, Královics R. 1996. Telomerase activity in plant cells. FEBS Lett, 391: 307-309.
[16]  Fitzgerald MS, McKnight TD, Shippen DE. 1996. Characterization and developmental patterns of telomerase expression in plants. Proc Natl Acad Sci USA, 93: 14422-14427.
[17]  Davar R, Darvishzadeh R, Majd A. 2011. Genotype-isolate interaction for resistance to Sclerotinia sclerotiorum in sunflower. Phytopathologia Mediterranea, 50: 442−449.
[18]  Amoozadeh, A. L, Darvishzadeh, R, Davar, R, Mandoulakani, B. A, haddadi, P, Basirnia, A. 2015. Quantitative trait loci associated with isolate specific and isolate non-specific partial resistance to Sclerotinia sclerotiorum in sunflower. Journal of Agricultural Science and Technology, 17 (1) : 213 - 226.
[19]  Schneiter AA, Miller JF, Kopp DD. 1981. Description of sunflower growth stages. Crop Science, 21: 901-903.
[20]  Schmittgen TDLivak KJ. 2008. Analyzing Real-Time PCR Data By the comparative C(T)  method. Nature Protocol, 3(6):1101-8.
[21]  Micic Z, Hahn, C BC. 2005. QTL mapping of resistance to Sclerotinia midstalk rot in RIL of sunflower population NDBLOSsel · CM625. Theor Appl Genet, 110: 1490–1498.
[22]  Hahn V. 2002. Genetic variation for resistance to Sclerotinia head rot in sunflower inbred lines. Field Crops Research, 77: 153–159.
[23]  Thomma BP, Eggermont K, Penninckx IA, Mauch-Mani B, Vogelsang R, Cammue BP, Broekaert WF. 1998. Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proc Natl Acad Sci USA, 95: 15107–15111.
[24]  Veronese P, Chen X, Bluhm B, Salmeron J, Dietrich R, Mengiste T. 2004. The BOS loci of Arabidopsis are required for resistance to Botrytis cinerea infection. The Plant Journal, 40: 558–574.
[25]  Xu H, Park NI, Li X, Kim YK, Lee SY. 2010. Park SU Molecular cloning and characterization of phenylalanine ammonia-lyase, cinnamate 4-hydroxylase and genes involved in flavone biosynthesis in Scutellaria baicalensis. Bioresource Technology, 101: 9715–9722.
[26]  Fitzgerald HA, Chern MS, Navarre R, Ronald PC. 2004. Overexpression of (At)NPR1 in rice leads to a BTH- and Environment-Induced Lesion-Mimic/Cell death phenotype. Molecular Plant-Microbe, 17: 140–151.
[27]  Liu F, Li X, Wang M, Wen J, Yi B, Shen J, Ma C, Fu T, Tu J. 2018. Interactions of WRKY15 and WRKY33 transcription factors and their roles in the resistance of oilseed rape to Sclerotinia infection. Plant Biotechnology Journal, 16: 911-925.
[28]  Hu X, Qin L, Roberts DP, Lakshman DK, Gong , Maul JE, Xie L, Yu C, Li Y, Hu L, Liao X, Liao X. 2017. Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT PCR and proteomic approach. BMC Genomics, 18: 674-688.
[29]  Oliveira MB, de Andrade RV, Grossi-de-Sá MF, Petrofeza S. 2015. Analysis of genes that are differentially expressed during the Sclerotinia sclerotiorum - Phaseolus vulgaris interaction. Frontiers in Microbiology, 26 ; 6:1162.