The effect of gamma rays on quinoa plant and evaluation of promising genotypes under salinity stress

Document Type : Research Paper

Authors

1 Genetic resource department, Desert Research Center

2 Nuclear Research Center, Atomic Energy Authority,

Abstract

There is no doubt that use of hybridization programs in the quinoa plant genotypes to induce genetic variation is difficult, however introducing the variations through mutation, to obtain promising genotypes, is much easier. In this research, quinoa seeds (Chipaya cv.) exposed to different doses of gamma rays and were cultivated in pots and open field under salinity stress. The results showed distinct differences at all studied traits in the native and mutant plants. Gamma ray’s irradiation caused genetic variations that was categorized based on studied traits, tolerance indices, cluster analysis of protein and ISSR data, which led to obtaining two promising mutations during M2. It should be noted that 90 and 120 Gy revealed the highest effects in producing desirable genetic variations. Also, the data resulting from the evaluation of phenotypic traits and tolerance indices of plants were confirmed by the biochemical and molecular analysis results. This research is providing new insights of using molecular breeding program for quinoa improvement to produce new promising genotypes powerfully face environmental stress and potential aid in future food shortage disasters.

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Main Subjects


[1] Abo-Elwafa A. & Bakheit B (1999): Performance, correlation and path coefficient analysis in faba bean.  Assiut Journal of Agriculture Science, 30: 77-91.
[2] Algosaibi A. M., El-Garawany M. M., Badran A. E.  Almadini A. M. (2015): Effect of irrigation water salinity on the growth of quinoa plant seedlings. Journal of Agricultural Science, 7: 205-214.
[3] Abd El. Hamid A., Abo Shosha A., Kassem M., El-Dabaawy E. (2014): Biotechnological studies on gamma irradiated Stevia (Stevia Rebaudiana) plant under abiotic stresses. In: Proc. 4thInt. Conf. Radiation Sciences and Applications, Taba, Egypt, October 13–17, 2014: 95-109.
[4] Blum A, Poyarkova H, Golan G, Mayer J (1983): Chemical desiccation of wheat plants as a simulator of postanthesis stress effects on translocation and kernel growth. Field Crops Research 6: 51-58.
[5] Cheema A., Atta M. (2003): Radio sensitivity Studies in Basmati Rice. Pakistan Journal of Botany 35: 197-207.
[6] Fernandez G (1992): Effective selection criteria for assessing plant stress tolerance. Proceedings of the International Symposium on Adaptation of Vegetables and other Food Crops in Temperature and Water Stress (Chapter 25, pp. 257-270), Taiwan.
[7] Laemmli U. K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227: 680-685.
[8] Lin C S, Binns M R (1988): A superiority measure of cultivar performance for cultivar location data. Canadian Journal of Plant Science 68: 193-198.
[9] Majidi M., Tavakoli V., Mirlohi A., Sabzalian M. (2011): Wild safflower species (Carthamus oxyacanthus): a possible source of drought tolerance for arid environments. Assiut Journal of Crop Science, 5:1055-1063.
[10 Maluszynsski M, Szarejko I, Bathia C, Nichterlein K, Lagoda P (2009): Methodologies for generating variability. Part 4: Mutation Techniques. In: Plant Breeding and Farmer Participation, Food and Agriculture Organization of the United Nations, Rome: 159 -194.
[11] Mastebroek, H. D., Limberg H., Gilles T., Marvin H. (2000): Occurrence of sapogenins in leaves and seeds of quinoa (Chenopodium quinoa Willd.). Journal of Science and Food Agriculture, 80:152-156
[12] Morillo C. A., Manjarres E. H., Morillo C. Y. (2017): Molecular characterization of Chenopodium quinoa Willd. using Inter Simple Sequence Repeat (ISSR) markers. African Journal of Biotechnology, 16:483-489.
[13] Rustikawati R., Suprijono E., Romeida A., Herison C., Sutjahjo S. (2012): Identification of M4 gamma irradiated maize mutant based on RAPD markers. Agrivita, 34: 161-165.
[14] Sarwat, M. (2012): ISSR; a reliable and cost effective technique for detection of DNA polymorphism. Methods in molecular biology, 862: 103-121
[15] Steel R., Torrie J. (1980): Principles and Procedures of Statistics: A Biometrical Approach. 2nd Ed. McGraw Hill Book Co. New York.
[16] Taheri S., Abdullah T., Ahmad Z., Sahebi M., Azizi P. (2016): Phenotypic and molecular effects of chronic gamma irradiation on Curcuma alismatifolia. Euro Journal of Horticulture Science, 81: 137-147.
[17] Vanhoudt N., Vandenhove H., Horemans N., Wannijn J., Hees M., Vangronsveld J., Cuypers A. (2010): The combined effect of uranium and gamma radiation on biological responses and oxidative stress induced in Arabidopsis thaliana. Journal of Environmental Radioactivity, 101: 923-930.
[18] Wi S., Chung B., Kim J., Baek M., Yang D., Lee J., Kim J. (2005): Ultra structural changes of cell organelles in Arabidopsis stem after gamma irradiation. Journal of Plant Biology 48:195-200.
[19] Yan W. (2001): GGEbiplot- a Windows application for graphical analysis of multi- environment trial data and other types of two-way data. Agronomy Journal, 93: 1111-1118.