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Mollaheydari Bafghi, R., Baghizadeh, A., Mohammadi-Nejad, G., Nakhoda, B. (2014). Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers. Journal of Plant Molecular Breeding, 2(1), 74-89. doi: 10.22058/jpmb.2014.8430
Robabeh Mollaheydari Bafghi; Amin Baghizadeh; Ghasem Mohammadi-Nejad; Babak Nakhoda. "Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers". Journal of Plant Molecular Breeding, 2, 1, 2014, 74-89. doi: 10.22058/jpmb.2014.8430
Mollaheydari Bafghi, R., Baghizadeh, A., Mohammadi-Nejad, G., Nakhoda, B. (2014). 'Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers', Journal of Plant Molecular Breeding, 2(1), pp. 74-89. doi: 10.22058/jpmb.2014.8430
Mollaheydari Bafghi, R., Baghizadeh, A., Mohammadi-Nejad, G., Nakhoda, B. Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers. Journal of Plant Molecular Breeding, 2014; 2(1): 74-89. doi: 10.22058/jpmb.2014.8430

Assessment of genetic diversity in Iranian wheat (Triticum aestivum L.) cultivars and lines using microsatellite markers

Article 7, Volume 2, Issue 1, Spring and Summer 2014, Page 74-89  XML PDF (2818 K)
Document Type: Research Paper
DOI: 10.22058/jpmb.2014.8430
Authors
Robabeh Mollaheydari Bafghi1; Amin Baghizadeh2; Ghasem Mohammadi-Nejad3; Babak Nakhoda4
1Department of Plant Breeding, Graduate University of Advanced Technology.
2Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman-Iran.
3Department of Agronomy and plant Breeding, College of Agriculture and Center of Excellence for Abiotic Stress in Cereal Crop., Shahid Bahonar University of Kerman-Iran.
4Department of System Biology , Agricultural Biotechnology Research Institute of Iran , P. O. Box, 31535-1897, Karaj –Iran.
Abstract
In this study, genetic diversity of 20 wheat genotypes was evaluated using 126 simple sequence repeats (SSR) alleles, covering all three wheat genomes. A total of 1557 allelic variants were detected for 126 SSR loci. The number of alleles per locus ranged from 4 to 19 and the allelic polymorphism information content (PIC) varied from 0.66 (Xgwm429) to 0.94 (Xgwm212 and Xgwm515). The high‌est polymorphism was observed in Xgwm212 and Xgwm515 primerswith 19 alleles, while the lowest polymorphism belonged to Xgwm429 with 4 alleles. The highest number of alleles per locus was detected in the genome A with 594 , compared to 552 and 411 for B and D  genomes, respectively. Dendrogram was constructed using Dice similarity coefficient and UPGMA algorithm by NTSYSpc2.0 software and genotypes were grouped in to six clusters. The knowledge about the genetic relationships of genotypes provides useful information to address breeding programs and germplasm resource management. This study also confirms the usefulness of SSR markers to study wheat genetic diversity.
Keywords
Genetic diversity; Microsatellite markers; Polymorphism; Wheat (Triticum aestivum L.)
Main Subjects
Molecular markers & plant breeding
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