Molecular markers & plant breeding
Asghar Estaji; Zahra Khazaei
Abstract
Tuber species are edible fungi and plant-symbiotic microorganisms that form a beneficial relationship with the roots of certain trees and plants (ectomycorrhizae). After interaction with a plant host, tuber species produced hypogeous fruit bodies of great economic value known as forest truffles. There ...
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Tuber species are edible fungi and plant-symbiotic microorganisms that form a beneficial relationship with the roots of certain trees and plants (ectomycorrhizae). After interaction with a plant host, tuber species produced hypogeous fruit bodies of great economic value known as forest truffles. There are different species of truffles, but based on species and place of origin varied their quality and market price. Truffle identification is based on morphological analysis maybe fail to distinguish them due to highly susceptible to environmental conditions. But using molecular markers to identify truffles can be more accurate, less expensive and reliable monitoring. In this context, twelve inter-simple sequence repeats (ISSR) primers were chosen for amplifying the genetic materials of black and brown truffles. In this study, a total of 57 polymorphic bands were amplified (an average of 5.18 bands). The Polymorphism Information Content (PIC) value and gene diversity (H) was with an average 0.37 and 0.50, respectively. During the ISSR screening good amplification products were obtained from primers based on GA, (AG) G, (AG)T, and GAC repeats. The population analysis result revealed that there are three main clusters A, B and C. Four strains Ardabil, Khalkhal, Zanjan and Urmia were identified to be in the group A cluster. The strains of at second and third groups were black and brown truffles respectively. The results indicated that truffles had two separate speciation events (DK = 2). According to DK = 2, the samples of Ardabil, Khalkhal, Zanjan and Urmia grouped in the same group and rest of truffles in other groups.
Germplasm genetic diversity & plant breeding
Alireza Ghanbari; Asghar Estaji; Somayie Fahim; Maryam Jamali
Abstract
The genus Crataegus spp. (Hawthorn) is belongs to Rosaceae family. The hawthorn can be utilized as a rootstock as well as an ornamental plant. The fruits are sources of many essential nutrients and have beneficial effects on human health. The Crataegus genus has been founded in different areas such as ...
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The genus Crataegus spp. (Hawthorn) is belongs to Rosaceae family. The hawthorn can be utilized as a rootstock as well as an ornamental plant. The fruits are sources of many essential nutrients and have beneficial effects on human health. The Crataegus genus has been founded in different areas such as Iran, especially in the Ardabil region. The collection and conservation of genetic material is important for future breeding programs. In this purpose, thirty hawthorn genotypes collected from several regions (Fandoghlo, Namin, Khalkhal, Aladizgeh, Germi) of Ardebil province and their genetic variations investigated with 15 ISSR markers. According to the results, all 15 primers amplified and produced total 128 bands (average = 8.53), as well as showed high polymorphism information content (average = %38.06). The results showed that there is a high level of genetic diversity and polymorphism ratio among the wild genotypes of hawthorn in the Ardabil region. Based on WARD technique, genotypes classified into three main clusters with several sub-clusters that exhibited high genetic diversity. The Germi genotypes completely isolated from the rest of the genotypes, which according to the structure analysis results, were similar to the Khalkhal genotypes.