Micropropagation & plant breeding
Mehdi Younessi-Hamzekhanlu; Ali Izadi-Darbandi; Mohammad Ali Malboubi; Mohsen Ebrahimi
Abstract
Direct regenerations by using mature cotyledonary node as a explants has been shown to be time-saving and convenient strategy for micropropagation of soybean. So we have evaluated regeneration protocol through single shoot using cotyledonary node as a rapid and efficient protocol for two soybean cultivars ...
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Direct regenerations by using mature cotyledonary node as a explants has been shown to be time-saving and convenient strategy for micropropagation of soybean. So we have evaluated regeneration protocol through single shoot using cotyledonary node as a rapid and efficient protocol for two soybean cultivars and one mutant line. Cotyledonary nodes explants obtained from 7-days-old in vitro seedlings. After 28 days, the percent of regeneration and after 42 days, regeneration area were calculated. The results showed that percent of regeneration and regeneration area of mutant line was significantly more than two cultivars, L17 and Williams. After shoot induction, plants were transferred to shoot elongation medium followed by transferring plants to rooting medium. The results showed that the percent of rooting was not significantly affected by genotypes. In another experiment to test kanamycin sensitivity of regenerated shoots, it was found that kanamaycin with 150 mg/L concentration is lethal for regeneration of soybean shoots from cotyledonary node explants. The results showed that regeneration efficiency of mutant line was significantly more than two other cultivars. Kanamaycin sensitivity of regenerated shoots showed that kanamycin at 150 mg/L or above can be used as a selective agent for all three tested cultivars transformation.
Molecular markers & plant breeding
Elham Mehrazar; Ali Izadi-Darbandi; Mohsen Mohammadi; Goodarz Najafian
Abstract
Marker assisted selection (MAS) is a tool for breeding, screening, and genetic characterization of germplasm. Allelic variation of both high and low molecular weight glutenin subunits (HMW/LMW-GS) is associated with the rheological properties of wheat flour. In this study, we investigated glutenin pattern ...
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Marker assisted selection (MAS) is a tool for breeding, screening, and genetic characterization of germplasm. Allelic variation of both high and low molecular weight glutenin subunits (HMW/LMW-GS) is associated with the rheological properties of wheat flour. In this study, we investigated glutenin pattern using SDS-PAGE and their PCR based on DNA markers in 60 advanced wheat lines and cultivars with different origins. Specific DNA markers regarding to Glu-1 loci, such as 1319 bp, 669 bp and 450 bp fragments were respectively validated for 2*, 17+18, 5+10 alleles. These alleles showed the highest allelic percentage in Glu-1 loci in studied cultivars. However the Null, 7+8 and 5+10 alleles showed the highest allelic percentage in advanced lines. In this study, 23%, 40% and 37% of cultivars respectively, got good (10), moderate (8-9) and weak (4-7) quality scores. In advanced lines, 18%, 44% and 38% got good, moderate and weak quality scores respectively. Ten specific DNA PCR markers were also detected for genotyping Glu-B3 alleles. The most frequent Glu-B3 alleles in wheat cultivars were i, a, b and d with 24%, 21%, 20% and 12%, respectively.Specific PCR markers regarding to the reported Glu-B3 alleles were produced as 621bp, 1095bp, 1570 bp and 662bp consequently. The most frequent Glu-B3 alleles in advanced lines belonged to a, i and d alleles with 35%, 26% and 21% respectively. The results provided useful information for breeding program to improve breadmaking quality and develop new cultivars.