Plant breeding for abiotic & biotic stresses
Nafise Taghizadeh; Gholam Ali Ranjbar; Ghorban Ali Nematzadeh; Mohammad Reza Ramazani Moghaddam
Abstract
Salinity is one of the most important limitation factors in development of agricultural products. Cotton has a relative tolerance to salinity; however, salinity reduces its growth during germination and seedling stages. In this research, split-factorial design of time based on randomized complete block ...
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Salinity is one of the most important limitation factors in development of agricultural products. Cotton has a relative tolerance to salinity; however, salinity reduces its growth during germination and seedling stages. In this research, split-factorial design of time based on randomized complete block design with 3 replications was used. The real-time PCR results for, root, stem, and leaves of 14-day cotton seedlings of tolerant (Sepid) and sensitive (Thermus14) cotton cultivars with salinity levels from 0 to 16 ds.m-1 were analyzed at three time points, namely 0, 7 and 14 days after salinity stress. Selected genes for Real Time PCR reaction in current study were selected using Cytoscape 3.3.0 software. Results showed that the selected genes GhERF2, GhMPK2, GhCIPK6, GbRLK, GhNHX1, GhGST, GhTPS1 and Gh14-3-3 have positively responded to salinity stress and their expression in the root was higher than in stem and leaf. Moreover, the expression of tolerant genotype (Sepid) was higher than the sensitive cultivar (Thermus 14) one, however, a slight increase in sensitive genotypes was observed in a number of genes (GhERF2 and GhGST) 14 days after starting the stress treatment.
Genetic engineering & plant breeding
Ebrahim Doranie Uliaie; Behzad Ghareyazi; Mohammad Farsi; Karl-Heinz Kogel
Abstract
A significant portion of the world’s cultivated land is affected by salinity that reduces crop productivity in these areas. Breeding for salt tolerance is one of the important strategies to overcome this problem. Recently, genetic engineering is becoming a promising approach to improving salt tolerance. ...
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A significant portion of the world’s cultivated land is affected by salinity that reduces crop productivity in these areas. Breeding for salt tolerance is one of the important strategies to overcome this problem. Recently, genetic engineering is becoming a promising approach to improving salt tolerance. In order to improve the yield performance of canola in saline soils, we transformed canola with Arabidopsis vacuolar Na+/H+ antiporter gene AtNHX1 which enhances the plant capacity for reducing cytosolic Na+ by transporting Na+ into the vacuole. Southern analysis of putative transgenic plants indicated that only one copy of the gene integrated into the plant genome. Overexpression of the AtNHX1 gene was shown in T1 transgenic plants. Under salinity conditions, stem and root length and overall biomass of transgenic plants were significantly higher compared to those of nontransgenic plants. Moreover, salt treated transgenic plants contained high proline and K+, but less Na+ compared to wild type.