Germplasm genetic diversity & plant breeding
Ayman Ebrahim Badran; Rasha M. Khalil; Ehab Mostafa
Abstract
In the context of the increasing demand for cultivation of marginal lands affected by salinity, it became necessary to assess the most important crop traits that can be relied upon as well as to identify genotypes that have the potential to withstand adverse environmental conditions. In this study ten ...
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In the context of the increasing demand for cultivation of marginal lands affected by salinity, it became necessary to assess the most important crop traits that can be relied upon as well as to identify genotypes that have the potential to withstand adverse environmental conditions. In this study ten genotypes of safflower crop were evaluated under two salinity levels during two successful agricultural seasons. According to correlation and path coefficient analysis and positive direct effect of studied traits, 100 seed weight followed by No. of capitulum / plant in related to seed yield. Also, the results confirmed based on tolerance indices to salt stress, that Line 6 was followed by local cultivar recorded the highest values as an indicator of tolerance. Protein analyses were conducted to identify protein markers associated with salinity tolerance for selection of promising Lines tolerant to salt stress. This investigation revealed high polymorphism with protein markers that could be useful tools to assist breeders in the selection and breeding of safflower Lines tolerant to salinity stress. Therefore, plant breeders can take advantage of these various parameters to design successful breeding program under conditions of salt stress.
Germplasm genetic diversity & plant breeding
Khadijeh Esmaeilnezhad; Mohammad Zare Mehrjerdi; Mahmoud Ghorbanzadeh Naghab
Abstract
Buxus hyrcana is one of the endangered and evergreen species of the Hyrcanian forests in Iran. The genetic diversity assessment is an essential step towards the conservation of this species. High-quality DNA is required for molecular markers analysis; therefore, we compared different DNA extraction methods ...
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Buxus hyrcana is one of the endangered and evergreen species of the Hyrcanian forests in Iran. The genetic diversity assessment is an essential step towards the conservation of this species. High-quality DNA is required for molecular markers analysis; therefore, we compared different DNA extraction methods on leaf samples of B. hyrcana. The quantity and quality of the extracted DNAs were evaluated by spectrophotometry and gel electrophoresis. Also, ISSR (Inter simple sequence repeats) markers were applied on the extracted DNAs to compare their quality for PCR amplification. Results showed that quantity, quality, and PCR efficiency and reproducibility were different for DNA extracted using different methods. The quality of the DNA at the absorbance A260/A280 ratio ranged from 1.02 to 1.97. The highest concentration of DNA measured by spectrophotometry belonged to the Cota-Sanchez extraction protocol (695.3 ng/ml) and the lowest value was obtained with Edward4 method (204.7 ng/ml). The modified Onate method (Onate2) was extracted the highest DNA concentration by comparison of brightness against the DNA ladder. Among the different extraction methods, the good quality and quantity were obtained in extracted DNA for Doyle and Doyle, Cota-Sánchez and modified Onate protocols; the latter method (Onate2) created both good quality and quantity of extracted DNA and operated effectively in terms of cost and time. Onate2 had the best amplification results with ISSR primers.
Germplasm genetic diversity & plant breeding
Nayyereh Ghorbani; Esmaeil Chamani; Ali akbar Shokouhian; seyyedeh sanaz Ramezanpour; Hassan Soltanlou
Abstract
Yellow flag (Iris pseudacorus) is a native plant with ornamental and medicinal properties in horticulture science. 16 ecotypes of I. pseudacorus species were collected and classified into three populations based on geographical location in the current study. The genetic diversity of I. pseudacorus was ...
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Yellow flag (Iris pseudacorus) is a native plant with ornamental and medicinal properties in horticulture science. 16 ecotypes of I. pseudacorus species were collected and classified into three populations based on geographical location in the current study. The genetic diversity of I. pseudacorus was assayed using 16 ISSR markers. Photosynthetic pigments, including chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids, were measured by the spectrophotometry method. The primers generated 874 scalable bars ranging in size from 100-1200 bp. The polymorphism percentage of all primers was 100%. The primers ISSR_55 produced the most bands (234 bands in total), the highest marker index, and the highest amount of polymorphic information content (PIC). Primer ISSR-13 is in second place with a total PIC of 0.84. Also, the data obtained from the scoring tapes were analyzed by parsing the original coordinates. The analysis results showed that the first, second, and third components contained 29.88%, 21.24%, and 16.52% of the information, respectively. The results showed that genetic diversity within populations (97%) is more significant than diversity among populations (3%). The spectrophotometry results showed photosynthetic pigments obtained in the Q (Jouybar) location with the highest sunlight. Our results indicated that ISSR markers revealed the genetic relationships of Yellow flag samples for different agro-ecological adaptations. ISSR is a superb molecular tool to research the genetic variability of I. pseudacorus.
Germplasm genetic diversity & plant breeding
Hossein Sabouri; Abdollatif Gholizadeh; Sharifeh Mohammad Alrgh; Somayyeh Sanchouli; Mahnaz Katouzi
Abstract
Rice is the world’s most important staple food and will continue to be so in the coming decades. Ferrous iron is essential for rice growth. A mapping population of 96 rice inbred lines derived by Neda (NAD) and Ahlemitarom (ATM) cross, was used to detect quantitative trait loci (QTLs) for fresh ...
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Rice is the world’s most important staple food and will continue to be so in the coming decades. Ferrous iron is essential for rice growth. A mapping population of 96 rice inbred lines derived by Neda (NAD) and Ahlemitarom (ATM) cross, was used to detect quantitative trait loci (QTLs) for fresh biomass (FB), root length (RL), shoot length (SL), root number (RN), leaf width (LW), root fresh weight (RFW), root dry weight (RDW) and Fe content (FC) under Fe toxicity condition in rice. Two parents and 96 inbred lines were evaluated for the traits by growing them under normal and Fe toxicity nutrient solution. Under stress condition, two QTLs were detected for FB on chromosome 10, with LOD of 2.859, and 2.465. Twelve QTLs were identified for RL on chromosomes 2, 4, 5, 6, 7, 8, 9, 10, and 12. Three QTLs were detected on chromosomes 6, 7, and 8 for RN, and two QTLs for RDW on chromosomes 2 and 9. One QTL controlling LW, RFW, and FC was located on chromosomes 10, 9, and 1, respectively. The other QTLs for FB, SL, and RN was located on chromosomes 12, 12, and 3 under normal condition, with respective contributions of 9.7, 10, and 9.9, respectively. qLWN-2, qLWN-7, and qLWN-12 were located for LW on chromosomes 2, 7, and 12. These QTLs, due to the high percentage of explanation after validation, are a good candidate for marker-assisted selection programs with the help of markers in the rice population.
Germplasm genetic diversity & plant breeding
Abdol reza Bagheri; Valiollah Ghasemi Omran; Mostafa Haghpanah
Abstract
Genetic relationships among twenty-three Chickpea genotypes include seven Chickpea cultivars, and 16 wild genotypes were analysed by 10 ISSR markers. On average, 11 bands per primer were observed, and 83.4 % polymorphic bands were shared in ISSR analysis. The results of clustering demonstrated the efficiency ...
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Genetic relationships among twenty-three Chickpea genotypes include seven Chickpea cultivars, and 16 wild genotypes were analysed by 10 ISSR markers. On average, 11 bands per primer were observed, and 83.4 % polymorphic bands were shared in ISSR analysis. The results of clustering demonstrated the efficiency of ISSR markers (particularly ISSR1 (UBC-834) and ISSR8 (UBC-864)) for reporting appropriate parents for breeding programs to biotic and abiotic stresses. Principle Coordinate Analysis (PCoA) clustered 23 Chickpea genotypes in two groups by which six cultivars separated in one group. Genetic data extracted from ISSR analysis are practical in Chickpea to choose parental sources for inbreeding programs.
Germplasm genetic diversity & plant breeding
Mohsen Saeidi; Majid Abdoli
Abstract
To evaluate the genetic diversity and the effect of drought stress on grain yield of wheat, 56 wheat genotypes were evaluated for terminal drought stress tolerance in field environments in the Kermanshah of Iran during the 2010-2011 cropping season. The experiments were conducted at the Campus of Agriculture ...
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To evaluate the genetic diversity and the effect of drought stress on grain yield of wheat, 56 wheat genotypes were evaluated for terminal drought stress tolerance in field environments in the Kermanshah of Iran during the 2010-2011 cropping season. The experiments were conducted at the Campus of Agriculture and Natural Resources, Razi University using alpha-lattice design with two replicates under two different water regimes included non-stress (normal irrigation at all stages of growth) and drought stress (end-season after flowering stage) conditions. Several new stress tolerance indices were evaluated. So that, ten drought tolerance indices including stress tolerance index (STI), relative drought index (RDI), yield index (YI), yield stability index (YSI), drought resistance index (DI), abiotic tolerance index (ATI), stress susceptibility percentage index (SSPI), sensitive drought index (SDI), modified stress tolerance index in normal irrigation (K1STI), and modified stress tolerance index in stress irrigation (K2STI) were calculated based on grain yield under drought (GYs) and irrigated (GYp) conditions. The result of analysis of variance indicated high significant differences among genotypes for grain yield trait. In general, terminal drought stress reduced 27.2% of grain yield. The Shiroudi, Rassoul, Darab-2, Marvdasht, Argh, and Shiraz genotypes which are high reduction of grain yield (61.1, 51.3, 48.4, 44.1, 43.1, and 43.0%, respectively) and also genotypes 318, Ghohar, 330, Mahdavi, and Alamout which are low reduction of grain yield with drought stress (4.1, 4.7, 7.0, 7.5, and 10.2%, respectively). Furthermore, results showed that wheat genotypes can be classified as normal and stress situations using cluster analysis. The correlation analysis among grain yield under non-stress and drought stress conditions with different drought tolerance indices showed that STI, YI, K1STI, and K2STI indices were appropriate indicators to identify the high grain yield genotypes. Based on these indicators, Mughan-1, Golestan, Navid, 330, Darab-2, and Bahar genotypes had the highest grain yield under both experimental conditions. Therefore, these wheat genotypes are suitable for cultivation in Mediterranean regions that are constantly exposed to drought stress at the end of the growing season, and areas with similar climatic conditions. Also, they are recommended to be used as parents for the improvement of drought tolerance in other wheat genotypes.
Germplasm genetic diversity & plant breeding
Habiballah Soughi; Kamal Payghamzadeh; Manoochehr Khodarahmi; Mehdi Nazari
Abstract
Wheat is one of the most important edible crops used in several food products. The success of plant breeding programs depends on the availability of parents with desired genetic diversities. In this context, we evaluated the genetic parameters in eight wheat genotypes namely Kohdashet, Morvaread, N-80-19, ...
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Wheat is one of the most important edible crops used in several food products. The success of plant breeding programs depends on the availability of parents with desired genetic diversities. In this context, we evaluated the genetic parameters in eight wheat genotypes namely Kohdashet, Morvaread, N-80-19, Darya, UR-82-17, Ehsan, Bacounoura and Atrack as well as theirs F1 progenies in term of grain yield and the relevant traits. Randomized complete block design with three replications was used. Data analysis indicated that genotypes and their F1 hybrids significantly affected grain yield, biological yield, thousand-grain weight, grains per spike, grain weight in spike, plant height, peduncle length and second internode length. The Model I of Griffing's method II was used in SAS software to determine GCA, SCA and other genetic parameters. Among parents, Ehsan cultivar exhibited the highest GCA for grain yield, thousand-grain weight, grain weight in spike, plant height and biological yield. P6×P7 cross was found to have the highest SCA effects on grain yield, thousand-grain weight and plant height. In addition, among examined characteristics, the thousand-grain weight and the peduncle length had the highest broad sense (h2= 0.98) and narrow sense heritability (H2= 0.62), respectively. These findings indicated that the distinctive crosses engaged in progenitors belongs to high×low GCA; therefore, it is inessential to set crosses with high×high GCA. As a result, potential homozygous lines can be selected from transgressive segregations to increase yield. Such crosses can be applied for exploiting heterosis.
Germplasm genetic diversity & plant breeding
Alireza Ghanbari; Asghar Estaji; Somayie Fahim; Maryam Jamali
Abstract
The genus Crataegus spp. (Hawthorn) is belongs to Rosaceae family. The hawthorn can be utilized as a rootstock as well as an ornamental plant. The fruits are sources of many essential nutrients and have beneficial effects on human health. The Crataegus genus has been founded in different areas such as ...
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The genus Crataegus spp. (Hawthorn) is belongs to Rosaceae family. The hawthorn can be utilized as a rootstock as well as an ornamental plant. The fruits are sources of many essential nutrients and have beneficial effects on human health. The Crataegus genus has been founded in different areas such as Iran, especially in the Ardabil region. The collection and conservation of genetic material is important for future breeding programs. In this purpose, thirty hawthorn genotypes collected from several regions (Fandoghlo, Namin, Khalkhal, Aladizgeh, Germi) of Ardebil province and their genetic variations investigated with 15 ISSR markers. According to the results, all 15 primers amplified and produced total 128 bands (average = 8.53), as well as showed high polymorphism information content (average = %38.06). The results showed that there is a high level of genetic diversity and polymorphism ratio among the wild genotypes of hawthorn in the Ardabil region. Based on WARD technique, genotypes classified into three main clusters with several sub-clusters that exhibited high genetic diversity. The Germi genotypes completely isolated from the rest of the genotypes, which according to the structure analysis results, were similar to the Khalkhal genotypes.
Germplasm genetic diversity & plant breeding
Ayman Ebrahim Badran; Rasha M. A. Khalil; Ezzat A. Kotb
Abstract
There is no doubt that use of hybridization programs in the quinoa plant genotypes to induce genetic variation is difficult, however introducing the variations through mutation, to obtain promising genotypes, is much easier. In this research, quinoa seeds (Chipaya cv.) exposed to different doses of gamma ...
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There is no doubt that use of hybridization programs in the quinoa plant genotypes to induce genetic variation is difficult, however introducing the variations through mutation, to obtain promising genotypes, is much easier. In this research, quinoa seeds (Chipaya cv.) exposed to different doses of gamma rays and were cultivated in pots and open field under salinity stress. The results showed distinct differences at all studied traits in the native and mutant plants. Gamma ray’s irradiation caused genetic variations that was categorized based on studied traits, tolerance indices, cluster analysis of protein and ISSR data, which led to obtaining two promising mutations during M2. It should be noted that 90 and 120 Gy revealed the highest effects in producing desirable genetic variations. Also, the data resulting from the evaluation of phenotypic traits and tolerance indices of plants were confirmed by the biochemical and molecular analysis results. This research is providing new insights of using molecular breeding program for quinoa improvement to produce new promising genotypes powerfully face environmental stress and potential aid in future food shortage disasters.
Germplasm genetic diversity & plant breeding
Aliakbar Babajanpour; SeyedHamidreza Hashemipetroudi; Mostafa Haghpanah
Abstract
Assessment of genetic diversity and individual relationships in rice (Oryza sativa) germplasm collections seems to be necessary for future rice breeding program. In order to understand genetic relationships of 30 rice genotypes, nine morphological traits, seven physicochemical properties and twelve RAPD ...
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Assessment of genetic diversity and individual relationships in rice (Oryza sativa) germplasm collections seems to be necessary for future rice breeding program. In order to understand genetic relationships of 30 rice genotypes, nine morphological traits, seven physicochemical properties and twelve RAPD primers were used for study of 30 rice genotypes. Among morphological traits, number of unfilled grain, number of tiller, number of filled grain and plant height had the highest CV value that indicated the high range of genetic diversity for studied genotypes. Pairwise correlation of morphological traits and physicochemical properties showed plant height had a strong positive correlation with panicle length (r = 0.721, P< 0.0001). Also, ratio of white rice to paddy rice and milling ratio had a negative correlation with plant height and 1000-grain weight, respectively. Cluster analysis of physicochemical properties and morphological traits grouped all genotypes into three main clusters. A total of 105 obtained RAPD bands, a number of 35 bands were polymorphs which range 7 to 19 bands per primer. OPB-14 and OPH-12 primers shown that lowest and the highest number of bands per primers, respectively. Cluster analysis of molecular data based on UPGMA algorithm and Jaccard's similarity coefficient grouped 30 rice genotypes into three clusters. The findings of this study might provide valuable information about local rice cultivar relationships in terms of their genetic distance, and can be useful in rice breeding program.
Germplasm genetic diversity & plant breeding
Shahrbanoo Abbasi; amin Baghizadeh; Ghasem Mohammadi-nejad
Abstract
In order to study the genetic conditions of some agronomic traits in wheat, a cross was made between Gaspard and Kharchia varieties. F2, F3 and F4 progenies with parents were evaluated under drought conditions. Three-parameter model [m d h] considered as the best fit for number of fertile tiller and ...
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In order to study the genetic conditions of some agronomic traits in wheat, a cross was made between Gaspard and Kharchia varieties. F2, F3 and F4 progenies with parents were evaluated under drought conditions. Three-parameter model [m d h] considered as the best fit for number of fertile tiller and flag leaf length using generations mean analysis method. For number of grain per spike and main spike grain weight three-parameter model [m d i] was used. For number of spikelet per spike, grain yield and plant height four-parameter model [m d h i] was used. The heritability values ranged from 56% for flag leaf length to 81% for grain yield. The F3 generation with 100 individuals was used to construct a genetic linkage map. Using the method of composite interval mapping 3, 1, 5, 2, 2 and 1 QTLs were detected for plant height, grain yield, number of spikelet per spike, flag leaf length, main spike grain weight and number of fertile tiller respectively.
Germplasm genetic diversity & plant breeding
Farnaz Goodarzi; Reza Darvishzadeh; Abbas Hassani
Abstract
Castor (Ricinus communis L.) is one of the most ancient medicinal oil crops in the world. It has been vastly distributed in different parts of Iran. In the present study, the inter simple sequence repeat (ISSR) markers were used to evaluate the molecular genetic diversity among and within 12 castor accessions ...
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Castor (Ricinus communis L.) is one of the most ancient medicinal oil crops in the world. It has been vastly distributed in different parts of Iran. In the present study, the inter simple sequence repeat (ISSR) markers were used to evaluate the molecular genetic diversity among and within 12 castor accessions collected from 7 regions of Iran. Totally, 16 ISSR primers amplified 166 loci, of which 116 loci (69.89 %) were polymorph, indicating high genetic variability in castor germplasm. An accession-specific ISSR band was detected in ‘80-29’accession. Genetic distance among accessions ranged from 0.2 to 0.056. Analysis of molecular variance revealed a higher level of genetic variation within (80%) than between (20%) accessions. A model-based Bayesian approach subdivided 60 genotypes from 12 accessions into 6 subgroups. UPGMA dendrogram based on Nei’s genetic distance classified 12 accessions into 4 groups. The result indicates that there was no association between geographical origin and ISSR patterns. The results suggest that ISSR technique is a useful tool for studying genetic diversity in castor germplasm.
Germplasm genetic diversity & plant breeding
Behzad Shahin Kaleybar; Sara Kabirnattaj; Ghorban Ali Nematzadeh; Seyyed Kamal Kazemitabar; Seyede Mona Salim Bahrami
Abstract
Rice as one of the most important agricultural crops has a putative potential for ensuring food security and addressing poverty in the world. In the present study, in order to provide basic information to improve rice through breeding programs, Inter Simple Sequence Repeat marker (ISSR) was used For ...
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Rice as one of the most important agricultural crops has a putative potential for ensuring food security and addressing poverty in the world. In the present study, in order to provide basic information to improve rice through breeding programs, Inter Simple Sequence Repeat marker (ISSR) was used For DNA fingerprinting and finding genetic relationships among 32 different cultivars. In this study, 12 out of 17 used primers amplified 184 distinct and reproducible fragments with high value of polymorphism (88%). also, for fingerprinting the cultivars 29 loci were used that generated high polymorphic bands among the cultivars. Results indicated that similarity index varied between 39% and 88.4 %, furthermore, PIC value with an average of 23% ranged between 0.1 (for primer #3) to 0.34 (for primer #2). Clustering based on Jaccard coefficient similarity index and UPGMA algorithm divided the cultivars into 6 main sub-clusters in cut-off point of 64% similarity index. The two Italian rice cultivars ‘Ribe’ and ‘Roma’ were the closest cultivars in addition, ‘Vialone nano’ and ‘Anbarbu’ showed the highest dissimilarity. In total, high genetic divergence was observed among the cultivars also, poly (GA)-containing 3-anchored primers amplified the highest number of bands. According to similarity and cluster analysis, it could be inferred that crosses involving Anbarbu cultivar are the most promising ones to improve rice through breeding programs. In fact, results of this study would be promising as a genetic marker for the identification of rice cultivars and an important source of knowledge for subsequent rice researches.