Study of genetic diversities and relatedness of Iranian citrus genotypes using morphological and molecular markers

Document Type : Original research paper


1 Department of Plant Breeding, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

2 Iran Citrus Research Institute, Ramsar, Iran.


Having knowledge about genetic relationships among accessions is necessary for developing breeding strategies to produce improved cultivars. In present study, genetic diversity and inter-relationship among 29 genotypes of citrus were comparatively analyzed using morphological and RAPD markers. Significant variability was observed among citrus genotypes for 61 quantitative and qualitative morphological characters of leaves, fruits and seeds. Furthermore, the RAPD markers revealed a high polymorphism rate (91.82 %). A pair-wise similarity value between genotypes ranged from 0.14 to 0.97 with average of 0.62. Both morphological and molecular analysis indicated a high degree of variation among studied genotypes. In current research, genotypes “pummelo” and “mandarin” were confirmed as true species of citrus in distinct cluster. Results of present study proved that both of morphological and molecular markers are potential tools for determining genetic diversities and genetic relationships of citrus genotypes and can be used in citrus breeding programs.


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Volume 3, Issue 1
June 2015
Pages 35-49
  • Receive Date: 26 April 2015
  • Revise Date: 26 August 2015
  • Accept Date: 26 August 2015
  • First Publish Date: 26 August 2015