The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.)

Document Type : Original research paper


1 Horticultural Department, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

2 Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.


Dog rose (Rosa canina L.) is one of the most important ornamental and medicinal plants which are used as a rootstock for ornamental roses such as Rosa hybrid and Rosa floribunda. In vitro propagation of rose has a very important role in rapid multiplication of species with desirable traits and in production of healthy and disease-free plants. Micropropagation of Rosa canina L. was revised, using its nodal segments under different combinations of BAP (0, 0.5, 1, 1.5 and 2 mgl1), GA3 (0 and 0.5 mgl1) and NAA (0 and 0.5 mgl1) on Murashige and Skoog (MS) and Van der Salm medium (VS) in proliferation stage and using different combinations of NAA and IBA (0, 0.3, 0.6 and 0.9 mgl1) and ½ VS medium in rooting stage. The highest shoot proliferation was obtained on VS medium containing 2 mgl1 BAP. Furthermore, the highest root induction obtained in ½ VS containing 0.6– 0.9 mgl1 of NAA or IBA. The present study presents an in vitro protocol for R. canina.


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Volume 3, Issue 1
June 2015
Pages 61-71
  • Receive Date: 26 August 2014
  • Revise Date: 26 August 2015
  • Accept Date: 26 August 2015
  • First Publish Date: 26 August 2015