Journal of Plant Molecular Breeding

Morphometric and zymogram patterns of peroxidase and superoxide dismutase enzymes analysis in populations of mosses in north of Iran

Document Type : Original research paper

Authors

1 Department of Plant Science, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran

2 Botanical Garden of Nowshahr, Research institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization, AREEO, Nowshahr, Iran

Abstract

The Hyrcanian forests have a remarkable variety of moss species which research on their taxonomy is of great importance. Since Forsstroemia remotifolia, Homalia besseri and Pseudoleskeella catenulata are exclusive and native mosses species of Hyrcanian forests, so in the current study, fourteen populations from three provinces in the north of Iran including Golestan, Mazandaran and Guilan were collected at the same altitudes in autumn 2017. In order to reveal the relationships among these species and populations, a cluster analysis based on numerical taxonomy and zymogram patterns of peroxidases and superoxide dismutase with Euclidean distances was performed. Numerical taxonomy analysis showed plant length, marginal laminal cell length and middle laminal cell length are appropriate traits to distinguish the species of F. remotifolia, H. besseri and P. catenulata from each other as well as their populations. The zymogram analysis showed genetic variability among species and also within populations of F. remotifolia, H. besseri and P. catenulata. Accordingly, the isozyme banding pattern of peroxidases showed a total of 6, 7 and 5 bands for F. remotifolia, H. besseri and P. catenulata, respectively. However, 4 isozyme bands were detected for superoxide dismutase for all three species. Furthermore, the morphological analyses in some populations was not matched with the isoenzyme banding pattern of enzymes in the current study. In conclusion, the biosystematics studies (morphometry and zymogram patterns of peroxidase and superoxide dismutase) indicate the close relationship between F. remotifolia and P. catenulata.

Keywords

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Journal of Plant Molecular Breeding
Volume 7, Issue 2
December 2021
Pages 1-11
  • Receive Date: 15 August 2020
  • Revise Date: 20 October 2020
  • Accept Date: 15 December 2020
  • First Publish Date: 15 December 2020