Only a few low molecular weight signals, including jasmonic acid (JA), salicylic acid (SA), and ethylene (ET), regulate the expression of a group of defense-related genes in the plants. The expression of plant defense genes is stopped against aggressive agents using these regulators, and in some cases, may be able to destroy or strengthen the defense signaling pathways of the plant for their purposes. In this study, the acetosyringone was used to study virulence genes induction in Agrobacterium tumefaciens A348 (MX311) and A348 (MX243) at three levels of 0, 100, and 200 μM. Subsequently, a concentration of this, which induced virulence genes highly, was selected to transform the Arabidopsis mutants using Agrobacterium tumefaciens EHA105. The results showed that the highest expression of virD2 and virB2 was at the levels of 200 and 0 μM of acetosyringone, respectively. Besides, the transformation results showed that the highest and lowest transformation efficiency was in the SA mutants (nahG) and the control plants (Col-0), respectively. Therefore, since the SA-suppression mutants have the highest transformation efficiency compared to others, it can be concluded that the SA may play more role in plant defense against pathogens than two other hormones. Hence, increasing the efficiency of gene transfer in these mutants will open the way for the expression and production of higher amounts of recombinant protein in these mutants compared to the wild type.