In vitro Plant Regeneration of Helianthus Annuus (Hyb. Azargol) From Alginate-Encapsulated Shoot Tips for Short Term Storage, Germplasm Exchange and Distribution

Document Type: Research Paper

Authors

1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

2 Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran

3 Dryland Agricultural Research Institute, Sararood, Kermanshah, Iran

Abstract

The present study demonstrates the potential of nutrient-alginate encapsulation of shoot tips of sunflower, Helianthus annuus (hyb. Azargol) for synthetic seed technology, which could be useful in germplasm distribution and exchange. Shoot tips from in vitro shoot cultures derived from mature seed explants were encapsulated in 3% sodium alginate and 100 mM CaCl2. 2H2O are supplemented with three different matrices (include distilled water, liquid MS medium and plant growth regulators) and they are stored for several periods (15, 30, 45 and 60 days) at 4°C. After each storage period for regeneration and regrowth evaluation, encapsulated and non-encapsulated shoot tips were cultured on hormone-free MS medium. The regrowth ability of encapsulated shoot tips affected by the storage duration and the presence or absence of MS nutrients in calcium alginate beads. Percentage response for the conversion of encapsulated and non-encapsulated shoot tips decreased gradually after storage at 4°C by increasing storage durations. Indeed, encapsulated vegetative propagules showed a higher resistance to storage at 4°C than non-encapsulated. Addition of MS nutrients in calcium alginate beads significantly improved encapsulated explants regrowth after storage periods.

Keywords

Main Subjects


[1]    Ara, H., Jaiswal, U. and Jaiswal, V. S. 2000. Synthetic seeds: prospect and limitations. Curr. Sci., 78: 1438-1444.

[2]    Badr-Elden, M. A. 2013. An Effective Protocol for in vitro Storage and ex vitro Regrowth of Strawberry Capsules. Atlas J of Chem Biochem., 1: 30-38.

[3]    Ballester, A., Janeiro, L.V. and Vieitez, A.M., 1997. Storage of shoot cultures and alginate encapsulation of shoot tips of Camellia japonica and C. reticulata Lindley. Sci. Hortic., 71: 67-78.

[4]    Bazinet, C., Kersulec, A., Dufrene, V., Timbert, R., Hervagault, J. F. and Barbotin, J. N. 1992. Physiological somatic embryos (Daucus carota L.) behavior depending on the storage conditions. Biotech, 92:139-146.

[5]    Castillo, B., Smith, M. A. L. and Yadava, U. L.  1998. Pant regeneration from encapsulated somatic embryos of Carica papaya L. Plant Cell Rep., 17: 172-176.

[6]    Chand, S. and Singh, A. K. 2004. Plant regeneration from encapsulated nodal segments of Dalbergia sissoo Roxb. A timber-yielding leguminous tree. J Plant Physiol., 161: 237-243.

[7]    Danso, K. E. and Ford-Lloyd, B. V. 2003. Encapsulation of nodal cuttings and shoot tips for storage and exchange of cassava germplasm. Plant Cell Rep., 21: 718-725.

[8]    Deglene, L., Lesignes, P., Alibert, G. and Sarrafi, A. 1997. Genetic control of organogenesis in cotyledons of sunflower (Helianthus annuus L.). Plant Cell Tiss. Org. Cult., 48: 127-130.

[9]    Fernandez-Martinez, J. M., Perez-Vich, B., Velaso, L. and Dominguez, J. 2007.Breeding for specialty oil types in sunflower. Helia., 30: 75-84.

[10] Fiore, M. C., Tabacc, T. and Sunseri, F. 1997. High frequency of plant regeneration in sunflower from cotyledons via somatic embryogenesis. Plant Cell Rep., 16: 295-298.

[11] Freyssinet, M. and G. Freyssinet, 1988. Fertile plant regeneration from sunflower (Helianthus annuus L) immature embryos. Plant. Sci., 56: 177-181.

[12] Hegazi, G. A. E. 2011. Viability of encapsulated shoot tips of )Capparis orientalis Duh(. Nature and Science., 9: 223-228.

[13] Honda, Y., Mukasa, Y. and Suzuk, T. 2005.Traits of NuSunTM varieties of sunflower in Hokkaido. Japan. Plant Prod. Sci., 8: 461-464.

[14] Lata, H., Ehandra, S., Khan, I. A. and Elsohly, M. A. 2009. Propagation through alginate encapsulation of axillary buds of (Cannabis sativa L.) an important medicinal Plant. Physiol Mol Biol Plants., 15: 79-86.

[15] Mandal, A. K., A. Chatterji, A. K. and Dutte Gupta, S. 1995. Direct somatic embryogenesis and plantlet regeneration from cotyledonary leaves of safflower. Plant Cell Tiss. Organ. Cult, 43: 287-289.

[16] Murashige, T. and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiol. Plant., 15: 473-497.

[17] Naik, S. K. and Chand, P. K. 2006. Nutrient-alginate encapsulation of in vitro nodal segments of pomegranate (Punica granatum L.) for germplasm distribution and exchange. Sci. Hort., 108:247-252.

[18] Nieves, N., Lorenzo, J. C., Blanco, M-delos-A., Gonzalez, J., Peralta, H., Hernandez, M., Santos, R., Caoncepcion, O., Borroto, C. G., Borroto, E., Tapia, R., Martinez, M. E., Foundora, Z. and Gonzalez, A., 1998, Artificial endosperm of Cleopatra targenine zygotic embryos : A model for somatic embryo encapsulation. Plant Cell Tiss. Org. Cult., 54: 77-83.

[19] Pugliesi, C., Cecconi, F., Mandolfo, A. and Baroncelli, S. 1990. Plant regeneration and genetic variability from tissue culture of sunflower (Helianthus annuus L.). Plant Breeding, 106: 114-121.

[20] Rai, M. K., Asthana, P., Singh, S.K., Jaiswal, V.S. and Jaiswal, U. 2009. The encapsulation technology in fruit plants—a review. Biotech. Adv., 27: 671–679.

[21] Rai, M. K., Jaiswal, V. S. and Jaiswal, U. 2008a. Encapsulation of shoot tips of guava (Psidium guajava L.) for short-term storage and germplasm exchange. Sci. Hortic., 118: 33–38.

[22] Rai, M.K., Jaiswal, V.S. and Jaiswal, U. 2008b. Alginate-encapsulation of nodal segments of guava (Psidium guajava L.) for germplasm exchange and distribution. J. Hortic. Sci. Biotech., 83: 569-573.

[23] Redenbaugh, K., Fujii, J. A., Slade, D., Viss, P. and Kossler, M. 1991. Artificial seeds encapsulated embryos. In Bajaj YPS (ed) High technology and micro propagation. Biot.  Agri. Fores. Springer, Berlin Heidelberg New York. 17: 395-416.

[24] Redenbaugh, K., Slade, D., Viss, P. and Fuji, J. 1987. Encapsulation of somatic embryos in synthetic seed coats. Hort. Sci., 22: 803-809.

[25] Sarkar, D. and Naik, P.S. 1997. Nutrient-encapsulation of potato nodal segments for germplasm exchange and distribution. Biol. Plant., 40: 285-290.

[26] Sharma TR, Singh BM, Chauhan RS. 1994. Production of disease free encapsulated buds of Zingiber officinale Rosc. Plant Cell Rep., 13: 300-302.

[27] Singh, A. K. Varshney, R. Sharma, M. Agarwal, S. S. and Bansal, K. C. 2006b. Regeneration of plants from alginate-encapsulated shoot tips of (Withaniasom nifera L.). Dunal, a medicinallyimportant plant species. J Plant Physiol., 163: 220-223.

[28] Singh, A. K., Sharma, M., Varshney, R., Agarwal, S. S. and Bansal, K. C. 2006a. Plant regeneration from alginate- encapsulated shoot tips of Phyllanthus amarus Schum and Thonn, amedicinally important plant species. In Vitro Cell Devop., l42: 109-115.

[29] Singh, S. K., Rai, M. K., Asthana, P. and L. Sahoo. 2009b. Alginate- encapsulation of nodal segments for propagation, short term conservation and germplasm exchange and distribution of (Eclipta alba L.). Acta Physiol. Plant., 32: 607-610.

[30] Singh, S. K., Rai, M. K., Asthana, P., Pandey, S., Jaiswal, V. S. and Jaiswal, U. 2009a. Plant regeneration from alginate encapsulated shoot tips of (Spilanthes acmella L.). Murr. A medicinally important and herbal pesticidal plant species. Acta Physiol. Plant. 31: 649-653.

[31] Singh, S., Manoj K., Rai, P., Sarita Pandey, V. S. and. Jaiswal, U. 2010. Plant regeneration from alginate-encapsulated shoot tips of (Spilanthes acmella L.). Murr., a medicinally important and herbal pesticidal plant species. Acta Physiol. Plant., 31: 649-653.

[32] Soneji, J. R., Rao, P. S. and Mhatre, M. 2002. Germination of synthetic seeds of pineapple (Ananas comosus L. Merr.). Plant Cell Rep. 20: 891–894.

[33] Tsvetkov, I., Jouve, L. and Hausman, J. E. 2006. Effect of alginate matrix composition on regrowth of in vitro-derived encapsulated apical microcuttings of hybrid aspen. Biol. Plant., 50: 722-724.

[34] West, T. P., Ravindra, M. B. and Preece, J. E. 2006. Encapsulation, cold storage, and growth of (Hibiscus moscheutos.) nodal segments. Plant Cell Tiss Org., 87: 223-231.

[35] Zeynali, M., Maleki zanjani, B., Saba, J., Niaazkhani, M., Ghaderian, M., Eivazi, A. and Mousavi-Anzabi, S. H. 2013. In vitro plant regeneration from alginate-encapsulated somatic embryos of rapeseed (Brassica napus cv. Tallayeh). INT. J. Trad. Herb. Medic, 1: 13-18.