Genetic Linkage Map of Glu-D1 and Allelic Variation of HMW Glutenin Subunits in Some Iranian Bread Wheat genotypes

Document Type: Research Paper

Authors

1 Department of Plant Breeding, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran.

2 Department of Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

High-molecular weight (HMW (glutenin subunits are encoded by the Glu-1 loci (Glu-A1, Glu-B1 and Glu-D1 (on the long arms of chromosomes 1A, 1B and 1D. In the present study, we constructed genetic linkage map of Glu-D1and then investigated the allelic variation of HMW glutenin subunits at Glu-A1 and Glu-D1 gene loci in 30 Iranian genotypes using Functional markers. Glu-D1 was located at 50.8 cM on chromosome 1D and it was tightly linked to wPt-3743 marker (>1 cM). QTL analysis using composite interval mapping detected one significant QTL for grain yield (GY) on chromosome 1D. This QTL (QYld.abrii-1D) was located between wPt-3743 and Glu-D1 gene locus. Allelic variation of HMW glutenin subunits showed the most frequent alleles were the null allele at Glu-A1and Dx2+Dy12 alleles at Glu-D1loci. The frequency of Null alleles or 1 and 2* were 40% and 60% respectively. Only 9 genotypes included allelic combination of Dx5+ Dy10 and the rest of genotypes had Dx2+Dy12 in the Glu-D1 locus. According to the Nei's genetic diversity index, alleles at Glu-A1 locus have more dispersion in genotypes compared to Glu-D1 locus. The cluster analysis of data based on the Simple Matching coefficient and UPGMC methods, classified the genotypes into four groups. Six genotypes including: Bezostaya, Tajan, Navid, Karaj1, Neyshabour, and Golestan had Ax2* and Dx5+ Dy10 subunits at Glu-A1 and Glu-D1 gene loci. Identification of genotypes with suitable allelic combinations can be used in breeding programs, especially in hybridization. 

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[1]     Azadi, A., Mardi, M., Majidi, Hervan. E., Mohammadi, S.A., Moradi, F., Tabatabaee, M.T., Pirseyedi, S.M., Ebrahimi, M., Fayaz, F., Kazemi, M., Ashkani, S., Nakhoda, B. and Mohammadi-Nejad, GH. 2015. QTL mapping of yield and yield components under normal and salt stress conditions in bread wheat (Triticum aestivum L.). Plant Mol. Biol. Rep. 33(1):102–120.

[2]     Asadi, A., Akbari, M., Mohammadi, Y. and Hossaininia, G.H. 2010. Agricultural Wheat Waste Management in Iran. Australian Journal of Basic and Applied Sciences. 4(3):421-428.

[3]     Wieser, H. Food Microbiol.  Chemistry of gluten proteins. 24(2):115-9. DOI:

[4]     Payne, P.I., Holt, L.M. and Law, C.N. 1981. Structural and genetical studies on the high molecular-weight subunits of wheat gluten. Part I: Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet.60:229–239.

[5]     Jackson, E.A., Holt, L.M. and Payne, P.I. 1983. Characterization of high-molecular weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two dimensional electrophoresis and the chromosomal localization of their controlling genes. Theor Appl Genet.66 (1):29–37.  

[6]     Payne, P.I. 1987. Genetics of wheat storage proteins and the eVect of allelic variation on bread-making quality. Annu Rev Plant Physiol Plant Mol Biol. 38:141–153.

[7]     Liu, L., He, Z.H., Ma, W.J., Liu, J.J., Xia, X.C. and Pena, R.J. 2009. Allelic variation at the Glu-D3 locus in Chinese bread wheat and effects on dough properties, pan bread and noodle qualities. CEREAL RES COMMUN. 37(1):57-64.

[8]     Yan, Y.M., Hsam, S.L.K., Yu, J.Z., Jiang, Y. and Zeller, F.J. 2003. Allelic variation of the HMW glutenin subunits in Aegilops tauschii accessions detected by sodium dodecyl sulphate (SDS- PAGE), acid poly acrylamide gel and capillary electrophoresis. Euphytica. 130(3):377-385.

[9]     Ahn, J.H., Kang, C.S., Jeung, J.U., Baik, B.K., Peña, R.J. and Park, C.S. 2014. Effect of allelic variations at the Glu-D1, Glu-A3, Glu-B3 and Pinb-D1 loci on flour characteristics and bread loaf volume. International Food Research Journal. 21(3):1177-1185.

[10]  Giraldo, P., Rodriguez-Quijano, M., Simon, C., Vazquez, J.F. and Carrillo, J.M. 2010. Allelic variation in HMW glutenins in Spanish wheat landraces and their relationship with bread quality. SPAN J AGRIC RES. 8(4):1012-1023.

[11]   Rodriguez-Quijano, M., Lucas, R., Ruiz, M., Giraldo, P., Espi, A. and Carrillo, J.M. 2010. Allelic Variation and Geographical Patterns of Prolamins in the USDA-ARS Khorasan Wheat Germplasm Collection. Crop Sci. 50:2383–2391.

[12]  Liu, S., Chao, S. and Anderson, J.A. 2008. New DNA markers for high molecular weight glutenin subunits in wheat. Theor Appl Genet. 118:177.

[13]  Rohlf, F., 1997. NTSYS-Pc. Numerical taxonomy and multivariate analysis system version 2.02e. Exeter Software. New York.

[14]  Nei, M. 1973. Analysis of Gene Diversity in Subdivided Population. Proc. Nat. Acad. Sci. 70(12):3321-3323.

[15]  Yeh, F.C., Yang, R., Boyle, T. 1999. POPGENE, Version 1.32. Microsoft Window-Based Freeware for Population Genetic Analysis; University of Alberta: Edmonton, AB, Canada,

[16]  Ma, W., Zhang, W. and Gale, K. 2003. Multiplex-PCR typing of high molecular weight glutenin alleles in wheat. Euphytica 134(1):51–60.

[17]  Bushuk, W. 1997. Wheat breeding for end poduct use. In: Wheat: Prospects for global imrovments. Developments in Plant Breedind. 6:203-211. The Netherland: Kluwer Academic Publishers.

[18]  Rabinovich, S.V. 1989. Composition of high molecular weight glutenin subunits connected with good quality in spring wheats and its distribution in different countries of world in: A.E. Slinkard (ed), Proc. 9th Intl. Wheat Genetics Symp. Proc. 9th Intl. 4:254-256.

[19]  Knežević, D., Šurlan-Momirović, G. and Ćirić, D. 1993. Allelic variation at Glu-1 loci in some Yugoslav wheat cultivars. Euphytica. 69(1):89-94.

[20]  Shahnejat-Bushehri, A.A., Gomarian, M. and Yazdi-Samadi B. 2006. The high molecular weight glutenin subunit composition in old and modern bread wheats cultivated in Iran. Australian Journal of Agricultural Research. 57(10):1109-1114.

[21]  Shan, X.Y., Clayshulte, S.R., Haley, S.D. and Byrne, P.F. 2007. Variation for glutenin and waxy alleles in the US hard winter wheat germplasm. Journal of Cereal Science. 45(2):199-208.     

[22]  Nakamura, H. 2000. Allelic variation at high-molecular-weight glutenin subunit loci, Glu-A1, Glu-B1 and Glu-D1, in Japanese and Chinese hexaploid wheats. Euphytica. 112:187-193.

[23]   Zheng, S. 2007. Relationship of Glutenin Loci and Rye Translocations with Dough Mixing Properties of Wheat Grown in Colorado Environments. Dissertation. Colorado State University.

[24]  Izadi-Darbandi, A, Yazdi-Samadi, B., Shahnejat-Boushehri, A.A. and Mohammadi, M. 2010. Allelic variations in Glu-1 and Glu-3 loci of historical and modern Iranian bread wheat (Triticum aestivum L.) cultivars. Genetics.89:193-199.

[25]    Deng, Z., Hu, S., Zheng, F., Chen, J., Zhang, X., Chen, J., Sun, C., Zhang, Y., Wang, S. and Tian, J. 2013. Genetic dissection reveals effects of interaction between high-molecular-weight glutenin subunits and waxy alleles on dough-mixing properties in common wheat. J. Genet. 92(1):69-79.