Analysis of genetic diversity among male and female pistachio genotypes using Start Codon Targeted (SCoT) markers

Document Type: Research Paper

Authors

1 Department of Genetics and Crop Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan,Iran.

2 Department of Genetics and Crop Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran.

10.22058/jpmb.2019.108160.1183

Abstract

ABSTRACT
Precise investigation of genetic diversity by means of novel molecular tools has made it possible to identify the superior genotypes among various male and female pistachio populations. Cytogenetic studies have shed light on the possible presence of distinct sex chromosomes in male and female genotypes. In this study, 22 SCoT primers were used in order to investigate the genetic diversity of 22 male and 22 female pistachio genotypes. A total of 434 loci were replicated and 339 loci were polymorphism. The average value of Polymorphic Information Content (PIC), Marker Index (MI), and Resolving power (Rp) which were all calculated based on data derived from SCoT markers, ranged from minimum 10 %, 0.5, and 1, to maximum 31%, 11.40, and 17.86 subsequently. The maximum (%69) and minimum (%35) values of genetic similarity between genotypes, were calculated using Jaccard's similarity coefficient. The cluster analysis divided pistachio genotypes into six groups, and could efficiently differentiate the male and female genotypes. Analysis of molecular variance (AMOVA) classified the total diversity into intra- and inter- population diversities with a high genetic variations (92%) within populations. This study reveals that SCoT marker is a useful and valuable molecular tool to separate male and female pistachio populations and to determine the genetic diversity among the populations.

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