Document Type: Research Paper
Gonbad Kavous University, Iran
The present research aims to association and allelic diversity of linked microsatellite markers to grain quality QTLs of 84 rice genotypes. To this end, 9 microsatellite markers (RM540, RM539, RM587, RM527, RM216, RM467, RM3188, RM246, RM5461) were used in which a total of 61 alleles were identified with a mean of 6 alleles per locus. The polymorphism information content (PIC) varied from 0.542 (RM540) to 0.812 (RMU3188) for SSR markers. Cluster analysis was performed using UPGMA method and genotypes were divided into five groups. Furthermore, based on regression analysis, for rice grain quality properties in flooding conditions as long as drought stresses, 10 alleles were identified. Of these, four alleles with gelatinization temperature, an allele with a protein content under flooding conditions, and three alleles with protein content and three alleles with gelatinization temperature were related under drought stress. It should be noted that the RM216-C and RM5461-D alleles were commonly identified in several traits. The presence of common markers for traits is probably due to the consistency of chromosomal locus controlling these traits or pleiotropy. The resutls of this study may imply that the important identified alleles for example RM216-A for gelatinization temperature (R2=30.1 %) can be used in rice quality improvement programs.