Mutation breeding
Ghorban Ali Nematzadeh; Morteza Oladi; Ammar Afkhami Ghadi; Ammar Gholizadeh Ghara; Farhad Bagheri; Mojtaba Aghajani; Maryam Emami Ghara; Alireza Babaei; Masud Rahimi; Camran Mozafari; Reza Vojdan; Amir Ziaee; Ahmad Esfandiyari
Abstract
Mutation breeding can be a major procedure for plant improvement, and release of high yield, including quality characteristics. A rice mutation breeding program with five verities (local and improved varieties including Sang Tarom, Tarom Chaloci, Nemat, Sepidrood, and Khazar) conducted at GABIT experimental ...
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Mutation breeding can be a major procedure for plant improvement, and release of high yield, including quality characteristics. A rice mutation breeding program with five verities (local and improved varieties including Sang Tarom, Tarom Chaloci, Nemat, Sepidrood, and Khazar) conducted at GABIT experimental field since 2010-2018. Five rice varieties treated with Gama ray at 200 Gray via AEOI , then the segregating generations (M2-M9) cultivated for 7 years and agronomic traits evaluation were done through pedigree method. Finally, eleven M9 pure promising lines selected then planted in Randomized Complete Block Design (RCBD) for further analysis. The results have shown that M9-P10-37-1-1-1-1-1-1-1 line (of Nemat mutrant) with high yield (8.23 ton/ha), early maturity (123.67 days), including good physicochemical quality such as AC=18.4%, GC=98 mm and GT= 5.42 with aromatic scent having fgr genes marker with 257bp length. The yield comparison indicate that the line M9-P10-37-1-1-1-1-1-1-1 has 12.51% comparing to Shiroodi and 47.97% comparing to Tarom Hashemi, with the high quality aromatic scent (the same as local physicochemical characteristics and aromatic scent). This new mutant line registered and introduced as Roshan variety.
Mutation breeding
Mozhgan Abtahi; Ahmad Arzani
Abstract
Mutation induction is considered as an effective way to enrich plant genetic variation, particularly for traits with a very low level of genetic variation. This research was conducted to assess genetic variation induced by gamma radiation in M2 and M3 mutant lines of canola (Brassica napus L.) by SSR ...
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Mutation induction is considered as an effective way to enrich plant genetic variation, particularly for traits with a very low level of genetic variation. This research was conducted to assess genetic variation induced by gamma radiation in M2 and M3 mutant lines of canola (Brassica napus L.) by SSR and morphological characteristics and to identify useful mutants in terms of agronomic traits. Sixty-two mutant lines derived from gamma mutagenesis and their wild-type progenitors (‘RGS003’ and ‘Sarigol’ cvs) were used. Twenty-five polymorphic SSR primers were used in this study. Results of cluster analysis based on both morphological traits comprising plant height, days to flowering, days to maturity, number of pods/plant, number of seeds/pod, 1000-seed weight and seed yield/plant and SSR data revealed a separate grouping of mutant lines from control cultivars. SSR data analysis of mutant lines and controls demonstrated a considerable genetic variation among mutant lines, where 83% of primers generated polymorphic bands with 3.32 alleles per locus. The genetic distance calculated between mutant lines and their controls indicated a significant difference between mutant lines and controls. Although both morphological and SSR markers successfully discriminated mutant lines from controls, SSR primers could further discriminate between the mutant lines derived from the related cultivar. Mutant lines 24 derived from ‘RGS003’ and 16 and 26 from ‘Sarigol’ were considered as superior for breeding canola, which could be utilized in future genetic and breeding programs. Distinct classification of genotypes based on agromorphological and SSR data in the present study implies that morphological and SSR markers reflected different aspects of genetic variation among mutant lines.