Molecular responses of Phytophthora capsici-challenged cucumber (Cucumis sativus L.) plants as influenced by resistance inducer application

Document Type : Original research paper


1 Department of Biotechnology, Shahrood University of Technology, Shahrood, Iran

2 Molecular Genetics Department, Genetics and Agricultural Biotechnology Institute of Tabrestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran


Phytophthora species are considered as the major cause of several plant diseases resulting in huge yield losses in agricultural crops. Despite years of effort to develop Phytophthora resistance varieties, there is no reports of a resistant cucumber variety. In this study, the effect of concomitant application of potassium phosphite (KPhi) and chitosan on some physiological and molecular responses of Phytophthora capsici-challenged cucumber plants were investigated. Cucumber plants were treated with KPhi and/or Chitosan at different concentrations and were then inoculated with zoospores of P. capsici and leaf samples were collected at different time courses. Results showed that Guaiacol peroxidase (GPOD) enzymatic activity surged immediately at first and second days after pathogen inoculation with a peak in plants treated with 4 gL-1 KPhi 2 days after inoculation. Compared to GPOD, the highest superoxide dismutase (SOD) activity was observed in the same treatment but later at 5 days after inoculation. It was indicated that the activity of antioxidant enzymes was greatly influenced by application of either KPhi or chitosan while their activity was not remarkably enhanced in control plants. qPCR analysis revealed that the highest increase in glutathione peroxidase (gpx) gene expression was achieved in plants concomitantly treated with 4 gL-1 KPhi and 200 mgL-1 chitosan 5 days after inoculation. The findings of this study provide novel information regarding inducing mechanisms of KPhi and chitosan which may be effective in mitigating disease severity.


[1]     Andreu AB, Guevara MG, Wolski EA, Daleo GR, Caldiz DO. 2006. Enhancement of natural disease resistance in potatoes by chemicals. Pest Manage Sci. 62:162-168.
[2]     Bell, A.A., Liu, L., Davis, M.R.  and Subbarao V.K. 1998. Effects of chitin and chitosan on the incidence and severity of Fusarium yellows of celery. Plant Dis 82: 322-328.
[3]     Chang, C. C., Slesak, I., Jorda, L., Sotnikov, A., Melzer, M., Miszalski, Z., Mullineaux, P. M., Parker, J. E., Karpinska, B. and Karpinski, S. 2009.Arabidopsis chloroplastic glutathione peroxidases play a role in cross talk between photooxidative stress and immune responses. Plant Physiol. 150: 670-683.
[4]     Chen, M., Li K., Li, H., Song, C. P. and Miao, Y. 2017. The Glutathione Peroxidase Gene Family in Gossypium hirsutum: Genome-Wide Identification, Classification, Gene Expression and Functional Analysis. Sci. Rep., 7: 44-47.
[5]     Eshraghi, L., Anderson, J., Aryamanesh, N., Shearer, B., McComb, J., Hardy, G. E. S., O’Brien, P. A. 2011.Phosphite primed defence responses and enhanced expression of defence gene s in Arabidopsis thaliana infected with Phytophthora cinnamomi. Plant Pathol., 60: 1086-1095.
[6]     Faoro, F., Maffi, D., Cantu, D., Iriti, M. 2008. Chemical-induced resistance against powdery mildew in barley: the effects of chitosan and benzothiadiazole. Biocontrol, 53: 387-401.
[7]     Förster, H., Adaskaveg, J. E., Kim, D. H. and Stanghellini, M. E. 1998. Effect of Phosphite on Tomato and Pepper Plants and on Susceptibility of Pepper to Phytophthora Root and Crown Rot in Hydroponic Culture. Plant Dis 82: 1165-1170.
[8]     Groves, E., Howard, K., Hardy, G. and Burgess, T. 2015. Role of salicylic acid in phosphite- induced protection against Oomycetes; a Phytophthora cinnamomi Lupinus augustifolius model system. Eur J Plant Pathol. 141:559–569.
[9]     Guest, D.I. and Bompeix, G. 1990. The complex mode of action of phosphonates. Aus. Plant Pathol. 19: 113-115.
[10]  Janda, T., Szalai, G. and Paldi, E. 2005. Investigation of antioxidant activity of maize during in low temperature stress. J Plant Physiol, 49: 53-54.
[11]  Jia, X., Meng, Q., Zeng, H., Wang, W. and Yin, H. 2016. Chitosan oligosaccharide induces resistance to Tobacco mosaic virus in Arabidopsis via the salicylic acid-mediated signaling pathway. Sci Rep, 6: 26-31.
[12]  Liu, Z. J., Zhang, X. L., Bai, J. G., Suo, B. X., Xu, P. L. and Wang, L. 2009. Exogenous paraquat changes antioxidant enzyme activities and lipid peroxidation in drought-stressed cucumber leaves. Scien Hort 121: 138-143.
[13]  Loon, L. C. V. 1989. Stress proteins in infected plants. In: Plant-microbe interactions; molecular and genetic perspectives. Vol. 3, T. Kosuge & E.W. Nester (eds.). McGraw-Hill Publ.-Co., New York, USA, 198-237.
[14]  Machinandiarena, M. F., Lobato, M. F., Feldman, M. L., Daleo, G. R. and Andreu, A. B. 2012. Potassium phosphite primes defense responses in potato against Phytophthora infestans. J Plant Physiol 169.
[15]  Matheron, M. E. and J. C. Matejka. 1992. Effects of temperature on sporulation and growth of Phytophthora citrophthora and P. parasitica and development of foot and root rot on citrus. Plant Dis 76: 1103-1109.
[16]  Mofidnakhaei, M., Abdossi, V., Dehestani, A., Pirdashti, H. and Babaeizad, V. 2016. Potassium phosphite affects growth, antioxidant enzymes activity and alleviates disease damage in cucumber plants inoculated with Pythium ultimum. Arch Phytopathol Plant Protect. 49:207-221.
[17]  Moradi, N., Rahimian, H., Dehestani, A. and Babaeizad, V. 2016. Cucumber response to Sphaerotheca fuliginea: differences in antioxidant enzymes activity and pathogenesis-related gene expression in susceptible and resistant genotypes. J Plant Mol Breed. 4:33-40
[18]  Moret, A., Munoz, Z. and Garces, S. 2009. Control of powdery mildew on cucumber cotyledons by chitosan, J Plant Pathol, 91: 375-380.
[19]  Ozyigit, I.I., Filiz, E., Vatansever, R., Kurtoglu, K.Y., Koc, I., Ozturk, M.X and Anjum N.A. 2016. Identification and comparative analysis of H2O2-scavenging enzymes (ascorbate peroxidase and glutathione peroxidase) in selected plants employing bioinformatics approaches. Front Plant Sci, 7:1-23.
[20]  Percival, G.C. and Banks, J.M. 2014. Evaluation of plant defense activators for the potential control of Pseudomonas syringae pv. aesculi. Arboricult J. 36:76-88.
[21]  Rakha M. and Lu, S. 1999. Evaluation of Fosphite rates against Phytophthora root rot Disease on salvia, J Hort. Biotech., 18: 22-30.
[22]  Ramezani, M., Rahmani, F. and Dehestani, A. 2017. Comparison between the effects of potassium phosphite and chitosan on changes in the concentration of Cucurbitacin E and on antibacterial property of Cucumis sativus. BMC Complement Altern Med, 17: 295.
[23]  Ramezani, M., Rahmani, F. and Dehestani, A. 2017. Study of physio-biochemical responses elicited by potassium phosphite in downy mildew-infected cucumber plant. Arch Phytopath Plant Protect, 50: 540-554.
[24]  Redolfi, P. 1983. Occurrence of pathogenesis-related (b) and similar proteins in different plant species. Netherlands J Plant Pathol., 89: 245-254.
[25]  Shibuya, N. and E. Minami 2001. Oligosaccharide signaling for defense responses in plant. Physiol, Mol Plant Pathol., 59: 223-233.
[26]  Sudarshan NR, H. D., Knorr D. 1992. Antibacterial action of chitosan. Food Biotechnol., 6: 257-272.
[27]  Ton, J., Jakab, G., Toquin, V., Flors, V., Iavicoli, A., Maeder, M., Metraux, J. and Mauch-Mani, B. 2005. Dissecting the beta-aminobutyric acid-induced priming phenomenon in Arabidopsis. Plant Cell. 17:987-999
[28]  Vasyukova NI, Chalenko GI, Gerasimova NG, Perekhod EA, Ozeretskovskaya OL, Irina AV, Varlamov VP, Albulov AI.  2005.Chitin and chitosan derivatives as elicitors of potato resistance to late blight. Appl Biochem Microbiol. 36:372-376.
[29]  Walters, D.R., Ratsep, J. and Havis, N.D. 2013. Controlling crop diseases using induced resistance: challenges for the future. J Exp Bot. 64:1263-1280.
[30]  Wilkinson, C. J., Holmes, J. M., Dell, B. Tynan, K. M. McComb, J. A., Shearer, B. L. Colquhoun, I. J. 2001. Effect of phosphite on in planta zoospore production of Phytophthora cinnamomi. Plant Pathol., 50: 587-593.
[31]  Xing, K., Zhu, X., Peng, X. and Qin, S. 2015. Chitosan antimicrobial and eliciting properties for pest control in agriculture: a review. Agron Sustain Dev, 35: 569-588.
Volume 5, Issue 2
December 2017
Pages 1-10
  • Receive Date: 30 July 2017
  • Revise Date: 11 October 2017
  • Accept Date: 14 October 2017
  • First Publish Date: 01 December 2017