Genetic diversity assessment of thirty nine Coffea canephora accessions using EST-SSR markers

Document Type : Original research paper


1 Crop improvement Division, Cocoa Research Institute of Nigeria, Ibadan, Oyo State, Nigeria

2 Department of Crop, Soil and Pest Management, Federal University of Technology, Akure, Ondo State, Nigeria


Studies on genetic diversity prove knowledge on genetic variation that can be useful to improve on food security. Variations were found among the thirty-nine robusta coffee accessions with the use of expressed sequence tag (EST)-simple sequence repeat (SSR) markers. The EST-SSR markers used in this study identified a total number of 15 alleles with an average of 3.0 alleles per locus. Primer CESR02, had the highest polymorphic information content value was of 0.59, the highest genetic diversity value (0.66) and the highest number of alleles (4). The highest percentage polymorphism of 42.86 was detected in primers CESR04 and CESR05. The phylogenetic dendrogram grouped the accessions into main three groups and six subgroups. The most genetic distinct accessions were accession: 3, 26 and 30. The most genetically related accessions accounted for 43.59% and the most distinct accession recorded 2.56%. For the Principal coordinate analysis (PCoA), the least genetic similar accessions accounted for 20.51%, while 28.21% of the accessions were genetically similar, representing the highest grouping. The results of this study clearly demonstrated that EST-SSR markers could be utilized to identify and categorize coffee accessions proper understand the genetic diversity among robusta coffee accessions for further improvement and preservation.


Volume 11, Issue 1
January 2023
Pages 17-27
  • Receive Date: 14 May 2023
  • Revise Date: 22 October 2023
  • Accept Date: 19 December 2023
  • First Publish Date: 19 December 2023