Journal of Plant Molecular Breeding

Response of wheat to drought stress: focus on root and shoot nutrients, as well as leaf chlorophyll and glycine betaine

Document Type : Original research paper

Authors

Department of biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Abstract

Drought stress is one of the limiting factors for plant growth. To evaluate the effect of drought stress (0, 150, 250 g/L of Polyethylene glycol 6000 (PEG)) on the physiological characteristics of two wheat cultivars (‘Gonbad’ and ‘N8720’), a hydroponic experiment was conducted. A factorial experiment was used and arranged in a completely randomized design with three replications at the Pasteur Institute of Iran (North Research Center). The experimental results showed that the main effect of cultivars was significant for all studied traits except nitrogen and phosphorus in the stems (p ≤ 0.01). The main effect of drought stress, as well as the interaction effect of drought stress and cultivars were significant for all studied traits (p ≤ 0.01). The highest content of elements in root and shoot and the chlorophyll content was observed in N8720 cultivar under control treatment. Moreover, in N8720 cultivar, the amount of glycine betaine increased due to drought stress, reaching its maximum at 250 g/L PEG. The results of correlation analysis showed that there is a positive and significant correlation between all traits (p ≤ 0.01). The result of experiment showed that N8720 cultivar exhibited superior characteristics in terms of all studied traits.
 

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Main Subjects

Abbas, T., Rizwan, M., Ali, S., Adrees, M., Mahmood, A., Zia-ur-Rehman, M., Ibrahim, M., Arshad, M., and Qayyum, M.F. (2018). Biochar application increased the growth and yield and reduced cadmium in drought stressed wheat grown in an aged contaminated soil. Ecotoxicol Environ Saf 148: 825-833.
Ahmad, N., Malagoli, M., Wirtz, M., and Hell, R. (2016). Drought stress in maize causes differential acclimation responses of glutathione and sulfur metabolism in leaves and roots. BMC Plant Biol 16: 1-15.
Ahmad, Z., Waraich, E.A., Akhtar, S., Anjum, S., Ahmad, T., Mahboob, W., Hafeez, O.B.A., Tapera, T., Labuschagne, M., and Rizwan, M. (2018). Physiological responses of wheat to drought stress and its mitigation approaches. Acta Physiol Plant 40: 1-13.
Annunziata, M.G., Ciarmiello, L.F., Woodrow, P., Dell’Aversana, E., and Carillo, P. (2019). Spatial and temporal profile of glycine betaine accumulation in plants under abiotic stresses. Front Plant Sci 10: 230.
Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24(1): 1.
Aslam, M., Maqbool, M.A., Cengiz, R., Aslam, M., Maqbool, M.A., and Cengiz, R. (2015). Drought stress in maize (Zea mays L.) effects, resistance mechanisms, global achievements and biological strategies for improvement. Springer International Publishing.
Barak, S., Mudgil, D., and Khatkar, B. (2015). Biochemical and functional properties of wheat gliadins: a review. Crit Rev Food Sci Nutr 55(3): 357-368.
Bashir, M.A., Silvestri, C., Ahmad, T., Hafiz, I.A., Abbasi, N.A., Manzoor, A., Cristofori, V., and Rugini, E. (2020). Osmotin: a cationic protein leads to improve biotic and abiotic stress tolerance in plants. Plants 9(8): 992.
Bindraban, P.S., Dimkpa, C., Nagarajan, L., Roy, A., and Rabbinge, R. (2015). Revisiting fertilisers and fertilisation strategies for improved nutrient uptake by plants. Biol Fert Soils 51(8): 897-911.
Bista, D.R., Heckathorn, S.A., Jayawardena, D.M., Mishra, S., and Boldt, J.K. (2018). Effects of drought on nutrient uptake and the levels of nutrient-uptake proteins in roots of drought-sensitive and-tolerant grasses. Plants 7(2): 28.
Bowles, T.M., Barrios-Masias, F.H., Carlisle, E.A., Cavagnaro, T.R., and Jackson, L.E. (2016). Effects of arbuscular mycorrhizae on tomato yield, nutrient uptake, water relations, and soil carbon dynamics under deficit irrigation in field conditions. Sci Total Environ 566: 1223-1234.
Cardona, T., Shao, S., and Nixon, P.J. (2018). Enhancing photosynthesis in plants: the light reactions. Essays Biochem 62(1): 85-94.
Chapman, H.D., and Pratt, P.F. (1962). Methods of analysis for soils, plants and waters. Soil Sci 93(1): 68.
Choudhary, S., Wani, K.I., Naeem, M., Khan, M.M.A., and Aftab, T. (2023). Cellular responses, osmotic adjustments, and role of osmolytes in providing salt stress resilience in higher plants: Polyamines and nitric oxide crosstalk. J Plant Growth Regul 42(2): 539-553.
Cottenie, A. (1980). Methods of plant analysis. Soil and Plant Testing. FAO Soils Bulletin 38(2): 64-100.
Delshadi, S., Ebrahimi, M., and Shirmohammadi, E. (2017). Influence of plant-growth-promoting bacteria on germination, growth and nutrients’ uptake of Onobrychis sativa L. under drought stress. J Plant Interact 12(1): 200-208.
Figueroa, M., Hammond‐Kosack, K.E., and Solomon, P.S. (2018). A review of wheat diseases—a field perspective. Mol Plant Pathol 19(6): 1523-1536.
Filstrup, C.T., and Downing, J.A. (2017). Relationship of chlorophyll to phosphorus and nitrogen in nutrient-rich lakes. Inland Waters 7(4): 385-400.
Gomes, M.P., Le Manac'h, S.G., Maccario, S., Labrecque, M., Lucotte, M., and Juneau, P. (2016). Differential effects of glyphosate and aminomethylphosphonic acid (AMPA) on photosynthesis and chlorophyll metabolism in willow plants. Pestic 130: 65-70.
Grieve, C., and Grattan, S. (1983). Rapid assay for determination of water soluble quaternary ammonium compounds. Plant Soil 70: 303-307.
Guo, R., Shi, L., Jiao, Y., Li, M., Zhong, X., Gu, F., Liu, Q., Xia, X., and Li, H. (2018). Metabolic responses to drought stress in the tissues of drought-tolerant and drought-sensitive wheat genotype seedlings. AoB Plants 10(2): ply016.
Hamada, A., and El-Enany, A. (1994). Effect of NaCl salinity on growth, pigment and mineral element contents, and gas exchange of broad bean and pea plants. Biol Plant 36: 75-81.
Hepworth, C., Doheny‐Adams, T., Hunt, L., Cameron, D.D., and Gray, J.E. (2015). Manipulating stomatal density enhances drought tolerance without deleterious effect on nutrient uptake. New Phytol 208(2): 336.
Hosseini, S.J., Tahmasebi-Sarvestani, Z., Mokhtassi-Bidgoli, A., Keshavarz, H., Kazemi, S., Khalvandi, M., Pirdashti, H., Bovand, F., and Abassian, A. (2023). Do various levels of salinity change chlorophyll fluorescence, nutrient uptake, and physiological characteristics of Mentha ecotypes? Ind Crops Prod 203: 117199.
Hosseini, S.J., Tahmasebi‐Sarvestani, Z., Pirdashti, H., Modarres‐Sanavy, S.A.M., Mokhtassi‐Bidgoli, A., Hazrati, S., and Nicola, S. (2021). Investigation of yield, phytochemical composition, and photosynthetic pigments in different mint ecotypes under salinity stress. Food Sci Nutr 9(5): 2620-2643.
Jangid, K.K., and Dwivedi, P. (2016). Physiological responses of drought stress in tomato: a review. Int J Environ Agric Biotech 9(1): 53-61.
Kaur, G., and Asthir, B. (2017). Molecular responses to drought stress in plants. Biologia plantarum 61: 201-209.
Kazemi, S., Rafati Alashti, M., and Hosseini, S.J. (2021). Response of biochemical and physiological properties of camellia (Camelina sativa L.) to foliar application of calcium and silicon nanoparticles. Silicon: 1-12.
Keshavarz‐Mirzamohammadi, H., Tohidi‐Moghadam, H.R., and Hosseini, S.J. (2021). Is there any relationship between agronomic traits, soil properties and essential oil profile of peppermint (Mentha piperita L.) treated by fertiliser treatments and irrigation regimes? Ann Appl Biol 179(3): 331-344.
Khalvandi, M., Amerian, M., Pirdashti, H., and Keramati, S. (2021). Does co-inoculation of mycorrhiza and Piriformospora indica fungi enhance the efficiency of chlorophyll fluorescence and essential oil composition in peppermint under irrigation with saline water from the Caspian Sea? PLoS One 16(7): e0254076.
Khalvandi, M., Amerian, M., Pirdashti, H., Keramati, S., and Hosseini, J. (2019). Essential oil of peppermint in symbiotic relationship with Piriformospora indica and methyl jasmonate application under saline condition. Ind Crops Prod 127: 195-202.
Khalvandi, M., Siosemardeh, A., Bahramnejad, B., and Roohi, E. (2023). Protein pattern and physiological responses to drought stress in wheat landraces. Cereal Res Commun: 1-13.
Kumar, S., Sachdeva, S., Bhat, K., and Vats, S. (2018). Plant responses to drought stress: physiological, biochemical and molecular basis. Biotic and abiotic stress tolerance in plants: 1-25.
Kurepin, L.V., Ivanov, A.G., Zaman, M., Pharis, R.P., Hurry, V., and Hüner, N.P. (2017). Interaction of glycine betaine and plant hormones: Protection of the photosynthetic apparatus during abiotic stress.
Li, R., Zeng, Y., Xu, J., Wang, Q., Wu, F., Cao, M., Lan, H., Liu, Y., and Lu, Y. (2015). Genetic variation for maize root architecture in response to drought stress at the seedling stage. Breed Sci 65(4): 298-307.
Liang, B., Ma, C., Zhang, Z., Wei, Z., Gao, T., Zhao, Q., Ma, F., and Li, C. (2018). Long-term exogenous application of melatonin improves nutrient uptake fluxes in apple plants under moderate drought stress. Environmental and experimental botany 155: 650-661.
Mitra, G.N. (2015). Regulation of nutrient uptake by plants.
Nadeem, M., Li, J., Yahya, M., Sher, A., Ma, C., Wang, X., and Qiu, L. (2019). Research progress and perspective on drought stress in legumes: A review. Int J Mol Sci 20(10): 2541.
Nawaz, M., and Wang, Z. (2020). Abscisic acid and glycine betaine mediated tolerance mechanisms under drought stress and recovery in Axonopus compressus: a new insight. Sci Rep 10(1): 6942.
Nuttall, J., O'leary, G., Panozzo, J., Walker, C., Barlow, K., and Fitzgerald, G. (2017). Models of grain quality in wheat—A review. Field Crops Res 202: 136-145.
Osman, H.S. (2015). Enhancing antioxidant–yield relationship of pea plant under drought at different growth stages by exogenously applied glycine betaine and proline. Ann Agric Sci 60(2): 389-402.
Osmolovskaya, N., Shumilina, J., Kim, A., Didio, A., Grishina, T., Bilova, T., Keltsieva, O.A., Zhukov, V., Tikhonovich, I., and Tarakhovskaya, E. (2018). Methodology of drought stress research: Experimental setup and physiological characterization. Int J Mol Sci 19(12): 4089.
Pardo-Domènech, L., Tifrea, A., Grigore, M., Boscaiu, M., and Vicente, O. (2016). Proline and glycine betaine accumulation in two succulent halophytes under natural and experimental conditions. Plant Biosyst 150(5): 904-915.
Rabiei, Z., Hosseini, S.J., Pirdashti, H., and Hazrati, S. (2020). Physiological and biochemical traits in coriander affected by plant growth-promoting rhizobacteria under salt stress. Heliyon 6(10).
Rizwan, M., Ali, S., Ibrahim, M., Farid, M., Adrees, M., Bharwana, S.A., Zia-ur-Rehman, M., Qayyum, M.F., and Abbas, F. (2015). Mechanisms of silicon-mediated alleviation of drought and salt stress in plants: a review. Environ Sci Pollut Res 22: 15416-15431.
Roychoudhury, A., and Banerjee, A. (2016). Endogenous glycine betaine accumulation mediates abiotic stress tolerance in plants. Trop Plant Res 3(1): 105-111.
Salehi-Lisar, S.Y., and Bakhshayeshan-Agdam, H. (2016). Drought stress in plants: causes, consequences, and tolerance.
Salehi, A., Tasdighi, H., and Gholamhoseini, M. (2016). Evaluation of proline, chlorophyll, soluble sugar content and uptake of nutrients in the German chamomile (Matricaria chamomilla L.) under drought stress and organic fertilizer treatments. Asian Pac J Trop Biomed 6(10): 886-891.
Sallam, A., Alqudah, A.M., Dawood, M.F., Baenziger, P.S., and Börner, A. (2019). Drought stress tolerance in wheat and barley: advances in physiology, breeding and genetics research. Int J Mol Sci 20(13): 3137.
Seleiman, M.F., Al-Suhaibani, N., Ali, N., Akmal, M., Alotaibi, M., Refay, Y., Dindaroglu, T., Abdul-Wajid, H.H., and Battaglia, M.L. (2021). Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants 10(2): 259.
Siddiqui, M.H., Al-Khaishany, M.Y., Al-Qutami, M.A., Al-Whaibi, M.H., Grover, A., Ali, H.M., Al-Wahibi, M.S., and Bukhari, N.A. (2015). Response of different genotypes of faba bean plant to drought stress. Int J Mol Sci 16(5): 10214-10227.
Singh, M., Kumar, J., Singh, S., Singh, V.P., and Prasad, S.M. (2015). Roles of osmoprotectants in improving salinity and drought tolerance in plants: a review. Rev Environ Sci Biotechnol 14: 407-426.
Sohrabi, Y., Habibi, A., Mohammadi, K., Sohrabi, M., Heidari, G., Khalesro, S., and Khalvandi, M. (2012). Effect of nitrogen (N) fertilizer and foliar-applied iron (Fe) fertilizer at various reproductive stages on yield, yield component and chemical composition of soybean (Glycine max L. Merr.) seed. Afr J Biotechnol 11(40): 9599-9605.
Tadayyon, A., Nikneshan, P., and Pessarakli, M. (2018). Effects of drought stress on concentration of macro-and micro-nutrients in Castor (Ricinus communis L.) plant. J Plant Nutr 41(3): 304-310.
Thalmann, M., Pazmino, D., Seung, D., Horrer, D., Nigro, A., Meier, T., Kölling, K., Pfeifhofer, H.W., Zeeman, S.C., and Santelia, D. (2016). Regulation of leaf starch degradation by abscisic acid is important for osmotic stress tolerance in plants. Plant Cell 28(8): 1860-1878.
Wang, S., Lin, H., Saito, T., Ohkawa, K., Ohara, H., Jia, H., and Kondo, S. (2019a). Abscisic acid affects ethylene metabolism and carotenoid biosynthesis in Japanese apricot (Prunus mume Sieb. et Zucc.). Agri Gene 12: 100083.
Wang, Y., Zhang, B., Jiang, D., and Chen, G. (2019b). Silicon improves photosynthetic performance by optimizing thylakoid membrane protein components in rice under drought stress. Environ Exp Bot 158: 117-124.
Webber, H., Ewert, F., Olesen, J.E., Müller, C., Fronzek, S., Ruane, A.C., Bourgault, M., Martre, P., Ababaei, B., and Bindi, M. (2018). Diverging importance of drought stress for maize and winter wheat in Europe. Nat Commun 9(1): 4249.
You, L., Song, Q., Wu, Y., Li, S., Jiang, C., Chang, L., Yang, X., and Zhang, J. (2019). Accumulation of glycine betaine in transplastomic potato plants expressing choline oxidase confers improved drought tolerance. Planta 249: 1963-1975.
Zegaoui, Z., Planchais, S., Cabassa, C., Djebbar, R., Belbachir, O.A., and Carol, P. (2017). Variation in relative water content, proline accumulation and stress gene expression in two cowpea landraces under drought. J Plant Physiol 218: 26-34.
Zivcak, M., Brestic, M., and Sytar, O. (2016). Osmotic adjustment and plant adaptation to drought stress.
Journal of Plant Molecular Breeding
Volume 11, Issue 2
June 2023
Pages 39-54
  • Receive Date: 13 July 2023
  • Revise Date: 06 February 2024
  • Accept Date: 06 February 2024
  • First Publish Date: 06 February 2024