Document Type : Original research paper

Authors

• Manpreet Kour
• Lalita Kumari
• Supriya Sharma

Sher-e-Kashmir University of Agricultural Sciences &Technology of Kashmir (SKUAST), Jammu, India

Abstract

The study utilized F2:5 lines from a cross between Kafiav N Zagora and Pusa Karishma to tag genomic regions controlling primary branches in B. juncea. One hundred and thirty F2:5 plants were used to characterize primary branch numbers, resulting in two pools of 12 genotypes for high and low branches. The average number of primary branches for HPB and LPB were 12.16 and 4.50. 148 SSRs was used for parental polymorphism screening from which 14 SSRs were used for analysis of HPB and LPB bulks. Allelic data scored for 14 lines was tested using T-test analysis to understand relationships for primary branches with SSR markers and alleles. Two markers Ni2-C12 and Ni2-A11 were found to be strongly linked to the number of branches. Bioinformatic analysis located the markers within a 9 Mb region on chromosome B5 of B. juncea. The current study was able to locate the loci regulating the no. of primary branches on B. juncea's sub-genome chromosome B5. Before fine-mapping investigations to deconstruct the genomic region (between 55.9 and 64.9 Mb) of sub-genome chromosome B5 could be conducted, it would be crucial to note that these results would need to be verified in diverse genetic backgrounds.

Keywords

• Keywords: Brassica juncea
• primary branch number
• BSA
• SSR

Main Subjects

• Plant genetics and breeding